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We narrowed to 509 results for: otos

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Showing: 241 - 260 of 509 results

  1. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...Byrne BJ, Zolotukhin S. A simplified purification protocol for recombinant adeno-associated virus vectors...Addgene.org Check out the Viral Service Find Virus Protocols Browse Our Viral Vectors ...

  3. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...specificity of influence measurements by reducing photostimulation of the axons and dendrites of non-targeted...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

  4. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...RNA provided that target is upstream (5') of a protospacer adjacent motif (PAM) Cas9 endonuclease binding... RNA and a 3-base pair sequence known as the Protospacer Adjacent Motif or PAM. In order for dsDNA to ...

  7. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...Usually Destroyed Yes, Cpf1 cleaves 5' of the protospacer Multiplexing CRISPR-Cas9 options before Cpf1...Delivery Method Advantages Disadvantages References Yamamoto Lab Golden Gate Assembly transfection One vector...

  8. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...you’re planning to go this route, check out our protocol video on using restriction digests to analyze ...Plasmids 101 series Find more molecular biology protocols and tips Resources on Addgene.org Learn more...

  9. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...educational resources — including blog posts, protocols, guides, and collections — is a lot of material...plasmids and viral preps for catalog, blog posts and protocols for educational resources, and labs and institutions...

  10. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...from the difficulty of developing an optimized protocol - it’s a bit like Goldilocks and the Three Steps...extra-methodical approach" to the top of your protocols when embarking on your ChIP journey!  References...

  11. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...Besides being compatible with any vector, the SLIC protocol itself is flexible. For one thing, those 5′ overhangs...Resources at Addgene Ligation-Independent Cloning Protocol Gibson Assembly Blog Post Choosing a Molecular...

  12. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ... indels that occur during DNA synthesis, and protomoting or repressing protein expression. Methylases ...Resources on Addgene.org Check Out Our Plasmid Protocols Boost Your Molecular Biology Background ...

  13. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...well-suited for use in stem cells. (See the complete protocol at StemBook). Addgene: How are you now using this...TALEN on paper to a finished plasmid. With our protocol, it is feasible to produce genetically modified...

  14. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ... with most multi-labeling immunohistochemical protocols where either a directly labeled primary or a primary...secondary antibody. Compatible with multi-labeling protocols when other antigens are detected with either a...

  15. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...a proxy for autophagy. Cells are cultured with isotope-labeled amino acids for several hours to several...Protein Oligomerization Seeing Red: Simple GFP Photoconversion Light Sheet Fluorescence Microscopy Additional...

  16. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...engineers in your lab, you likely will need to write protocols, SOPs, and methods sections. The next time you...academic labs when creating shared experimental protocols. Clarify: Asking for clarification during a meeting...

  17. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    .... Use the "Contact info" link right below their photo. 2) Personalize all connection requests LinkedIn...for the picture. It tends to look like a police photo if you look straight at the camera. Pro tip: Turn...

  18. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...has  developed a proprietary, low cost cloning protocol that he has used for cloning of more than 10,000...plasmid elements Resources on Addgene.org Find protocols for plasmid cloning Read our molecular biology...

  19. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ... RNA editing. Fast facts – PAM requirement: protospacer flanking sequence – A, U, or C. Best for: targeting...development and use. Fast facts – PAM requirement: protospacer flanking sequence – none. Best for: targeting...

  20. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ... saves time and money when developing assays.  Protocol architecture The basic (very basic!) architecture...increased binding affinity, many indirect antibody protocols are designed to take place over multiple days....
Showing: 241 - 260 of 509 results