We narrowed to 509 results for: otos

...specificity of influence measurements by reducing photostimulation of the axons and dendrites of non-targeted...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

...Byrne BJ, Zolotukhin S. A simplified purification protocol for recombinant adeno-associated virus vectors...Addgene.org Check out the Viral Service Find Virus Protocols Browse Our Viral Vectors ...

... the workshop, the participants took part in a protocol drawing exercise. The workshop also used a shared...different parts of the poster. Conferences, such as Photonics Online Meetup, hosted their poster session on ...

...RNA provided that target is upstream (5') of a protospacer adjacent motif (PAM) Cas9 endonuclease binding... RNA and a 3-base pair sequence known as the Protospacer Adjacent Motif or PAM. In order for dsDNA to ...

...verification to find additional tips and detailed protocols on topics such as how to set up your digests and...Addgene's Molecular Biology Reference Find More Protocols and Videos      ...

...educational resources — including blog posts, protocols, guides, and collections — is a lot of material...plasmids and viral preps for catalog, blog posts and protocols for educational resources, and labs and institutions...

...from the difficulty of developing an optimized protocol - it’s a bit like Goldilocks and the Three Steps...extra-methodical approach" to the top of your protocols when embarking on your ChIP journey!  References...

...2017): 113-117. PubMed PMID: 28225754. 6. Marumoto, Tomotoshi, et al. "Development of a novel mouse glioma...

...Usually Destroyed Yes, Cpf1 cleaves 5' of the protospacer Multiplexing CRISPR-Cas9 options before Cpf1...Delivery Method Advantages Disadvantages References Yamamoto Lab Golden Gate Assembly transfection One vector...

...Besides being compatible with any vector, the SLIC protocol itself is flexible. For one thing, those 5′ overhangs...Resources at Addgene Ligation-Independent Cloning Protocol Gibson Assembly Blog Post Choosing a Molecular...

...you’re planning to go this route, check out our protocol video on using restriction digests to analyze ...Plasmids 101 series Find more molecular biology protocols and tips Resources on Addgene.org Learn more...

... indels that occur during DNA synthesis, and protomoting or repressing protein expression. Methylases ...Resources on Addgene.org Check Out Our Plasmid Protocols Boost Your Molecular Biology Background ...

...well-suited for use in stem cells. (See the complete protocol at StemBook). Addgene: How are you now using this...TALEN on paper to a finished plasmid. With our protocol, it is feasible to produce genetically modified...

...engineers in your lab, you likely will need to write protocols, SOPs, and methods sections. The next time you...academic labs when creating shared experimental protocols. Clarify: Asking for clarification during a meeting...

.... Use the "Contact info" link right below their photo. 2) Personalize all connection requests LinkedIn...for the picture. It tends to look like a police photo if you look straight at the camera. Pro tip: Turn...

...has  developed a proprietary, low cost cloning protocol that he has used for cloning of more than 10,000...plasmid elements Resources on Addgene.org Find protocols for plasmid cloning Read our molecular biology...

... RNA editing. Fast facts – PAM requirement: protospacer flanking sequence – A, U, or C. Best for: targeting...development and use. Fast facts – PAM requirement: protospacer flanking sequence – none. Best for: targeting...

... saves time and money when developing assays.  Protocol architecture The basic (very basic!) architecture...increased binding affinity, many indirect antibody protocols are designed to take place over multiple days....

... with most multi-labeling immunohistochemical protocols where either a directly labeled primary or a primary...secondary antibody. Compatible with multi-labeling protocols when other antigens are detected with either a...

...excitable. The mutants are co-expressed with a photoconvertible fluorescent protein mEOS4a. Cells containing...sensor that shows a favorable outcome are then photoconverted, sorted, and sequenced to identify the mutants...