...of the method. One observation was an inverse relationship between deletion size and frequency [3]. There... DNA function is typically inferred based on correlative biochemical characteristics and ectopic reporter...   Like the video? The Orkin and Bauer lab's related paper describes their methods for CRISPR design...

...level of control over timing. Because chemogenetics relies on chemical activation and the diffusion of drugs...throughout the body. Controlling Stimulation Some studies rely on delivering reversible or varied amounts of stimulation... of being less invasive and more flexible as it relies on designer ligands that can be simply introduced...

...treatment can browse through screens performed in relevant cell lines or conditions. This increased ease ...screen name takes users to a display page with the relevant metadata and the screen results. Screenshots updated...enzyme system, sgRNA library and other details relevant to screen result interpretation. The analytical...

...preparation approaches described previously require a preliminary double stranding step. The double stranding step...preparations and showed that VGS can quickly and reliably confirm the identity of AAV genomes and detect...the Addgene blog Read all of our viral vectors related blog posts Read our blog post on AAV titers Browse...

...all of your data prepared. All of these questions relate to factors that you should have already recorded...Fluorescence? Immunohistochemistry? Did you use any relevant animal treatment conditions like drugs (Chemogenetics...reproducible research. Share your data on AAV data hub! Related resources on the Addgene blog Important Considerations...

...viral vectors. Here are some of the AAV we have released in the last few months. You can also browse our...that govern such activities as neurotransmitter release is vital in studying neural networks. We have expanded...Dopamine and norepinephrine Neuroscience research relies on accurate monitoring of neurotransmitters and...

...are often well-equipped. Many institutions are sincerely interested in connecting with the public in a ... this subject allowed me to engage in findings relevant to my background (neuroimaging, biomechanics, ... health policy implications arising from sport-related concussion.   More resources on the Addgene blog...

... cells (Vandenberghe, 2010). Rather than being released into the cell culture supernatant, the viral particles...wide range of downstream applications scientists rely on our AAV preparations for, we wanted to be certain..., Luk H., et al. "Efficient serotype-dependent release of functional vector into the culture medium during...

...post-PCR procedures. Sounds easy right? The technique relies on the basic ability of complementary basepairs...covalent bond thus joining the two DNA strands and releasing topoisomerase (2).  These days commercially available...extra salt to your reaction: Topoisomerase I is released from the vector when the PCR product and vector...

...been an exciting year for the Addgene blog. We’ve released over 80 posts on a diverse array of topics pertinent...Plasmids 101 2nd ed, the 3rd edition has now been released), and have started providing recorded interviews...making a transition into a new job or maybe to an entirely new career path, a career coach might be able ...

...no matter how well you know your idea, you will rarely succeed in launching a company without partners...platform for significant company advancement. Be relentless The world is against you.  As I mentioned, you... you’re able to answer every possible question related to it. First impressions are everything and you...

...the virus interacts with in cells. Because viruses rely on the help of human proteins in their lifecycle...other cell types, including more physiologically-relevant systems, to catch all the important interactions...and Facebook. Find more COVID-19 and coronavirus related plasmids and resources at Addgene* Many thanks...

... of ApoB-LPs has been shown to have a strong correlation with cardiovascular disease and indirect measurement...that the system is active in E. coli and closely related species, is amenable to use with other bEBP/UAS...and the system may not be adaptable to distantly related species from E. coli. This tool should serve as...

..., check out this related blog post: Early Career Researcher Toolbox: Finding Relevant Papers. These are...audience. The tool highlights jargon terms or words rarely used in general writing in red, words with a medium...

...Zhang lab’s previously published protocol and its relevant modifications on the pooled library webpage. Disease... that any researcher could use to generate the relevant disease model. Accurate modeling of a disease ...isoforms. Due to the size of the collection, we’re releasing individual plasmids in batches. If there’s something...

...measured using a red calcium sensor) leads to the release of serotonin (measured using the newly developed...developed green serotonin sensor). In preliminary studies with the lab’s collaborators, they were able to use...use the serotonin sensor in sleep-related studies and in assays for measuring human serotonin transporter...

...chairs in the hallway for people to take a break and relax. Companies at LabCentral get access to a variety... this is the most important part of this newly released prep kit. In their prior NEBNext Ultra II DNA ...understand? How does the information I’ll be learning relate to me as an individual and Addgene as a company...

..., and they can rupture E. coli spheroplasts to release encapsulated proteins. Video by ACS. For a more...Tunable Protein Piston That Breaks Membranes to Release Encapsulated Cargo. ACS Synth. Biol. (2016). doi...contain microtubule-like structures are bacteria related to Verrucomicrobia. PNAS 97, 1813–1817 (2000). ...

...performances, and research experiences, you now have merely two pages to convince your potential employer that...when the research topic you have done is closely related to the job you are applying for, in which case ...in Experimental Medicine. She is the Community Relations Director for Curiosity Collider (hyperlink https...

...that contain your plasmid backbone, as it often relies on antibiotic resistance. But what about identifying...method of screening is the blue-white screen, which relies on the lacZ gene. lacZ encodes the enzyme 𝛽-galactosidase... functional 𝛽-galactosidase. Blue-white screens rely on vectors where the gene of interest is inserted...