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We narrowed to 628 results for: rel

Showing: 241 - 260 of 628 results

  1. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...All plasmids rely on their host cell's replication machinery in order to replicate—but not always to ...replication independently are said to be under relaxed control, as they only require the host's replication...replication (oriC), where initiation begins. This process relies on the initiation protein DnaA. First, DnaA must... chromosomal DNA ensure that the helicases are released from the DNA once the replication forks reach ...

  2. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...researchers and publishers. Nonetheless, the initial release caused a storm as the organization left open the... data be stored in a retrievable format, we too rarely preserve “raw” data of any sort in a form that ...is important to my lab is three-fold. Firstly, we rely on many other labs to teach us how to do experiments...product. If we don’t take interest in how it is released, we will be in danger of having to deliver on ...

  3. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...to avoid animal use by using semi-synthetic or entirely synthetic VL and VH sequences. Phage display also...scFvs are also used for eye diseases, such as age-related macular degeneration (AMD) since direction injection...of the CAR facilitates signal transduction and release of cytotoxic granules from the T cell. When a CAR... CAR has an scFv that binds cancer-related antigens, binding of the scFv part of the receptor can activate...

  4. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...is low, but since all downstream experiments will rely on that clone not having any confounding mutations...sequencing The only way to quantify off-target effects entirely is to perform whole genome sequencing. While this...major con is that binding by Cas does not always correlate with catalytic activity at those sites, especially...and integrase-defective lentiviral vectors - which rely on insertion of DNA by the NHEJ pathway during DNA...

  5. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ... of CRISPR technologies, one might incorrectly relegate RNAi to history. The straightforwardness of RNAi...2018) and the model bryophyte Physcomitrium (Physcomitrella) patens (Bezanilla et al., 2005; Nakaoka et...Klueh P, Quatrano RS (2005) An RNAi System in Physcomitrella patens with an Internal Marker for Silencing...2012) An Inducible RNA Interference System in Physcomitrella patens Reveals a Dominant Role of Augmin in...

  6. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...specific sites that allow them to be cleaved and released. These constructs tend to be smaller than constructs... If Csy4 is not expressed, the gRNAs cannot be released, adding temporal and/or spatial control to the...recognize the tRNA sequences, cleave them, and release the gRNAs. PTGs are assembled into a wt Cas9-containing...editing of six different genes. This system doesn’t rely on any additional or host-dependent factors and ...

  7. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...) after three years on hold, Addgene staff may release the hold unless otherwise requested. Distribute...assembled or theoretical — it does not have to be entirely verified by sequencing. Upload any support files...easier for other scientists to find information related to your plasmid. It is optional to include the ...and alternative name boxes as needed. Use the "Relevant mutations/deletions" box to describe any mutations...

  8. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...produced less reliable results. She also notes that for reasons that are not yet entirely clear some PUF...1;8(1):7. doi: 10.1186/1754-1611-8-7. PubMed.  Related Resources Addgene's Synthetic Biology Overview...

  9. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ...of the method. One observation was an inverse relationship between deletion size and frequency [3]. There... DNA function is typically inferred based on correlative biochemical characteristics and ectopic reporter...   Like the video? The Orkin and Bauer lab's related paper describes their methods for CRISPR design...

  10. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...level of control over timing. Because chemogenetics relies on chemical activation and the diffusion of drugs...throughout the body. Controlling Stimulation Some studies rely on delivering reversible or varied amounts of stimulation... of being less invasive and more flexible as it relies on designer ligands that can be simply introduced...

  11. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    Published
    Jan. 21, 2020, 2:30 p.m.
    ...treatment can browse through screens performed in relevant cell lines or conditions. This increased ease ...screen name takes users to a display page with the relevant metadata and the screen results. Screenshots updated...enzyme system, sgRNA library and other details relevant to screen result interpretation. The analytical...

  12. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ... cells (Vandenberghe, 2010). Rather than being released into the cell culture supernatant, the viral particles...wide range of downstream applications scientists rely on our AAV preparations for, we wanted to be certain..., Luk H., et al. "Efficient serotype-dependent release of functional vector into the culture medium during...

  13. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    Published
    April 23, 2020, 1:15 p.m.
    ...preparation approaches described previously require a preliminary double stranding step. The double stranding step...preparations and showed that VGS can quickly and reliably confirm the identity of AAV genomes and detect...the Addgene blog Read all of our viral vectors related blog posts Read our blog post on AAV titers Browse...

  14. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ...all of your data prepared. All of these questions relate to factors that you should have already recorded...Fluorescence? Immunohistochemistry? Did you use any relevant animal treatment conditions like drugs (Chemogenetics...reproducible research. Share your data on AAV data hub! Related resources on the Addgene blog Important Considerations...

  15. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...are often well-equipped. Many institutions are sincerely interested in connecting with the public in a ... this subject allowed me to engage in findings relevant to my background (neuroimaging, biomechanics, ... health policy implications arising from sport-related concussion.   More resources on the Addgene blog...

  16. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...viral vectors. Here are some of the AAV we have released in the last few months. You can also browse our...that govern such activities as neurotransmitter release is vital in studying neural networks. We have expanded...Dopamine and norepinephrine Neuroscience research relies on accurate monitoring of neurotransmitters and...

  17. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...post-PCR procedures. Sounds easy right? The technique relies on the basic ability of complementary basepairs...covalent bond thus joining the two DNA strands and releasing topoisomerase (2).  These days commercially available...extra salt to your reaction: Topoisomerase I is released from the vector when the PCR product and vector...

  18. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...been an exciting year for the Addgene blog. We’ve released over 80 posts on a diverse array of topics pertinent...Plasmids 101 2nd ed, the 3rd edition has now been released), and have started providing recorded interviews...making a transition into a new job or maybe to an entirely new career path, a career coach might be able ...

  19. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...no matter how well you know your idea, you will rarely succeed in launching a company without partners...platform for significant company advancement. Be relentless The world is against you.  As I mentioned, you... you’re able to answer every possible question related to it. First impressions are everything and you...

  20. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...chairs in the hallway for people to take a break and relax. Companies at LabCentral get access to a variety... this is the most important part of this newly released prep kit. In their prior NEBNext Ultra II DNA ...understand? How does the information I’ll be learning relate to me as an individual and Addgene as a company...
Showing: 241 - 260 of 628 results