We narrowed to 263 results for: rigi

1. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

   Type

   Blog Post

   Published

   Oct. 11, 2016, 2:30 p.m.

   ...Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the...

2. CRISPR 101: Epigenetics and Editing the Epigenome

   Type

   Blog Post

   Published

   June 24, 2020, 5:45 p.m.

   ...Originally published Feb 14, 2017 and updated Jun 24, 2020. Epigenetic modifications are an additional...

3. Viral Vectors 101: AAV Serotypes and Tissue Tropism

   Type

   Blog Post

   Published

   Jan. 16, 2025, 2:15 p.m.

   ...for contributing their expertise! This post was originally published in January 2025 and updated in August...

4. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

   Type

   Blog Post

   Published

   April 5, 2018, 12:42 p.m.

   ... a standard dsDNA repair template is used. The original design for creating a conditional allele, or floxed...

5. Recombinase-based State Machines Enable Order-dependent Logic in vivo

   Type

   Blog Post

   Published

   July 28, 2016, 2:30 p.m.

   ...Input Plasmid: The input plasmid (high copy, ColE1 origin of replication), contains large serine recombinase...

6. PiggyBac-ing Through the Genome Editing Field

   Type

   Blog Post

   Published

   May 31, 2016, 3:30 p.m.

   ...piggyBac™ transposon? The piggyBac™ transposon was originally identified in moths in 1983, but wasn’t successfully...

7. Plasmids 101: Using Transposons in the Lab

   Type

   Blog Post

   Published

   July 6, 2021, 1:28 p.m.

   ...Tol2 or miniTol2 is a truncated version of the original Tol2 and has ~3-fold increase in transposition...

8. A Guide to Getting Started in Undergrad Research

   Type

   Blog Post

   Published

   June 8, 2021, 1:15 p.m.

   ...their undergraduate years. We each have a unique “origin story,” but taking the first step into the world...

9. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

   Type

   Blog Post

   Published

   Dec. 17, 2020, 1:30 p.m.

   ...Originally published Jan 27, 2015 and last updated Dec 17, 2020. This post was contributed by Jordan ...

10. When Fidelity Matters: A frank discussion about ligase fidelity

    Type

    Blog Post

    Published

    March 6, 2018, 1:49 p.m.

    ...can reliably detect single-base differences. The original creators of this technique visualized the ligation...

11. Transferable Skills Guide: Teamwork

    Type

    Blog Post

    Published

    Nov. 28, 2017, 2 p.m.

    ...Next Great Discovery is exactly that: a myth. The original Dr. Frankenstein from Mary Shelley’s novel worked...

12. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type

    Blog Post

    Published

    Dec. 15, 2016, 3:57 p.m.

    ... red genes from a plasmid with a heat sensitive origin of replication.  Once the recombineering system...

13. Custom CRISPR Screens & the Green Listed Software

    Type

    Blog Post

    Published

    July 11, 2017, 2:30 p.m.

    ...use, including all information provided in the original reference library, as well as GC%, reverse compliment...

14. Live and Let Dye: Self-Labeling Protein Tags

    Type

    Blog Post

    Published

    April 16, 2024, 1:15 p.m.

    ...Gautier et al., 2008). Although based on the same original enzyme, SNAP-tag and CLIP-tag show good specificity...

15. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type

    Blog Post

    Published

    March 21, 2024, 1:15 p.m.

    ...nanoparticles, maintaining intensity at ~85% of its original value after 60 minutes of illumination, highlighting...

16. Cancer and the Immune System: Deciphering the Relationship

    Type

    Blog Post

    Published

    March 14, 2017, 2:30 p.m.

    ...especially immune cells and cells of epithelial origin. Suppressing function, creating tolerance, and ...

17. CRISPR 101: Multiplex Expression of gRNAs

    Type

    Blog Post

    Published

    Sept. 10, 2020, 11:45 a.m.

    ...Originally published Jan 28, 2016 and last updated Sep 10, 2020 by Jennifer Tsang. CRISPR makes it easy...

18. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type

    Blog Post

    Published

    Nov. 6, 2018, 1:12 p.m.

    ...specific to the lineage of the target cell in the original somatic cells [41]. The in vivo and in vitro methods...

19. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type

    Blog Post

    Published

    Sept. 3, 2020, 12:15 p.m.

    ...This blog post was originally written by Caroline LaManna, published Mar 8, 2016. The updated and expanded...

20. Delivery Methods for Generating iPSCs

    Type

    Blog Post

    Published

    April 17, 2018, 1:37 p.m.

    ...EBNA1) based episomes: These plasmids carry the origin of replication (oriP) element and the cis-acting...