Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 599 results for: REV;

Showing: 131 - 140 of 599 results

  1. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ... functional copy of your plasmid of interest, preventing this issue. The genes and their antibiotics   ...produces beta-lactamase to degrade amp and therefore prevent cell death. One of the cool things about amp is...susceptible to degradation in agar plates. This prevents satellite colonies from forming while effectively...expressing a protein that directly binds to zeocin, preventing it from binding DNA. Zeocin is an all-in-one ...

  2. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...within a CRISPR guide can prevent the guide from binding to the genome or prevent Cas9 from cutting, both...genome could affect their analysis. Their work revealed that cell lines from individuals of African descent...Perturbation Platform to modify the CRISpick algorithm to prevent guides from being designed that target regions ...

  3. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...there are several parallel mechanisms in place (for review see Maday et al., 2014). It is currently unclear...neurotoxicity, and is lethal if left untreated (for review see Dietzschold et al., 2008). Herpes Simplex Virus...neuronal connection via a single synaptic step, thus preventing further widespread expansion of RABV. This improvement...combination with conditional transgenic mice, to reveal not only the anatomy of neuronal connections, but...Cell type-specific genetic and optogenetic tools reveal novel hippocampal CA2 circuits. Nat Neurosci 17...

  4. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...a different mechanism - the PhoCl protein is irreversibly cleaved when exposed to light. Robert Campbell...marker-free coselection, analogous to methods previously used in C. elegans, targets the sodium/potassium... the point mutations created by Agudelo et al. prevent ouabain binding to ATP1A1 but do not compromise...for TZAP, a Kruppel-like zinc finger protein (previously named ZBTB48) in regulating the upper-bound of...engineering that enables new applications, such as reversing the effects of dCas9 binding to a genomic locus...

  5. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...we give people the opportunity to speak out and prevent groupthink? Here are some ideas that I have found...a member of our Business Development team would review it. This survey generated 26 ideas, over half of...mistakes that kill your productivity. Harvard Business Review. Retrieved January 14, 2022, from https://hbr.org... spur innovation on your team. Harvard Business Review. Retrieved January 14, 2022, from https://hbr.org...

  6. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ... the Wisconsin research community were to: (1) prevent vendor lock in by making it easy to get data out... to a selected Slack channel for discussion and review. It’s also possible to capture Slack conversations... and/or import and convert it into RSpace. The reverse flow is also supported, so it’s possible to re-...making research data available for public access, review, and query. Gates Open Research and Wellcome Open...

  7. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ... limited to 0-5%. This blog post covers a brief review of different basic BE technologies and their adaptation...making an original C-G pair converted to T-A or reverted to the original C-G by base excision repair mediated... by introducing a point mutation (H193Y). The reversion of this mutation by an adenine base editor would...in rice. In Genome Engineering: The CRISPR-Cas Revolution, Cold Spring Harbor Laboratory (2018). Additional...

  8. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...participant in the training. In this blog we will review the preparation and planning you can do to build...different way of doing something then you’ve done previously) you can bring those questions to your training...questions throughout training, while others prefer to review their notes, process what they’ve just learned,...Show up prepared and take training seriously, as reviewed above. Offer to help clean up and/or make necessary...

  9. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...correlated with codon bias in both E. coli and S. cerevisiae. Protein folding - If a protein is encoded by...Gene optimization mechanisms: A multi‐gene study reveals a high success rate of full‐length human proteins..."Evolutionary conservation of codon optimality reveals hidden signatures of cotranslational folding." ...26186290. PubMed Central PMCID: PMC4794256. This review provides a great overview of codon usage bias ...

  10. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...containing the desired edit adjoined to the sgRNA, a reverse transcriptase, and a nCas9. While this system has... Fig. 2: Prime editor engaging target DNA. The reverse transcriptase polymerizes DNA onto the target strand...successfully in experiments and published in peer reviewed journals. If you need to generate a new sgRNA ...your experiment. Keep an eye out for our upcoming review of these options in our CRISPR 101 series!  References...
Showing: 131 - 140 of 599 results