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  1. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    Published
    July 6, 2017, 1:59 p.m.
    ...Cancer and the Immune System: Deciphering the Relationship” by our guest blogger Subhadra Jayaraman, a ... 113-117. PubMed PMID: 28225754. 6. Marumoto, Tomotoshi, et al. "Development of a novel mouse glioma model...Cancer and the Immune System: Deciphering the Relationship Resources on Addgene.org Browse Cancer Plasmids...
  2. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...?) As a result, I’d always end up stressed and rushing, no matter how hard I tried to build in extra time...especially on days when I was tired, stressed, or rushing. I would spend the time quietly running through... consider it this way: When you’re stressed or rushing, you’re much more likely to make a mistake. When...
  3. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...mCyRFP1 include a redshifted-emission spectrum compared to that of CyOFP1, a large stokes shift, long fluorescence...s properties. pBAD-LSSmCherry1 is a long Stokes shift variant, which could be compatible with two-photon... Ti-Sapphire lasers. pBAD-RDSmCherry1 is a red-shifted variant which, with further development, could ...
  4. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    .... PubMed Central PMCID: PMC4517063. 3. Mizushima, N., Yoshimori, T., & Levine, B. (2010). Methods in Mammalian...Pho8 phosphatase activity or the molecular weight shift of Pho8 from its uncleaved to cleaved form is the...
  5. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ...Therapeutics Graduate Program has a compulsory internship to be taken in an industry, regulatory, or clinical...many notable contributions in this area include publishing the book ‘How Economics Shapes Science’ and contributing...employ them, the diversity of trainee backgrounds, publishing from a young scientist’s perspective, and the...
  6. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...plate with every plasmid or as individual plasmids shipped as agar stabs. Iverson, et al. ACS Synth Bio. ...likelihood of creating a functional knock-out via frameshift and minimize off-target effects. The library ...Destination series, pCAG-Golden-Gate-Esp3I-Destination Takashi Yamamoto pcDNA-TAL-NC2, pCAGGS-TAL-NC2 Charles... et al. Cell Rep. 2016. PubMed PMID: 27052166 Nishimura K, et al. Nat. Methods 2009. PubMed PMID: 19915560... prototrophy, can also aid in designing self-establishing metabolically cooperating (SeMeCo) communities...
  7. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...all of your plasmid data, we’ll send you prepaid shipping materials (i.e., a deposit kit) with instructions...we need to know your Addgene account username. Shipping address and phone number. Plasmid sequences or...deposit kit with instructions on how to prepare and ship the samples to Addgene and contact your technology...
  8. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...01039-7 Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., ...disrupting its canonical structure and Cas9 binding (Nishimasu et al., 2014). Figure 3: pegRNAs whose...
Showing: 131 - 140 of 457 results