We narrowed to 513 results for: EMB

1. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

   Type

   Blog Post

   Published

   June 1, 2021, 1:15 p.m.

   ...is a science writer, former scientist, and proud member of Crowdfight. He is currently based in Spain and...

2. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

   Type

   Blog Post

   Published

   June 30, 2020, 1:15 p.m.

   ...polling feature or the chat box. One key thing to remember regardless of the type of session you’re presenting...

3. Scientists Map the SARS-CoV-2-Human Interaction Network

   Type

   Blog Post

   Published

   April 9, 2020, 1:15 p.m.

   ...shipped out of the Krogan lab at UCSF. Our team members (shout out to Margaret Soucheray, Jeff Guo, Paddy...

4. Plasmids 101: NGS Quality Control for Pooled Libraries

   Type

   Blog Post

   Published

   Oct. 26, 2017, 1:59 p.m.

   ...libraries. Here are the key points you’ll need to remember when undertaking library amplification and sequencing...

5. What Good Citizenship Can Do for Reproducibility in Science

   Type

   Blog Post

   Published

   Nov. 7, 2019, 2:18 p.m.

   ...including Cell Metabolism, Cell Reports and Joule. In September 2017 she moved away from direct editorial work...

6. Networking Follow-up: I Met a Person. Now What?

   Type

   Blog Post

   Published

   Jan. 12, 2021, 2:15 p.m.

   ...Kamen's LinkedIn network! The connections between members of her network give rise to a starfish shape. ...

7. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

   Type

   Blog Post

   Published

   March 24, 2016, 2:30 p.m.

   ...investigating DNA repair pathways.     Additonal Corn Lab Members who worked on this publication:         References...

8. Synthetic Photobiology: Optogenetics for E. coli

   Type

   Blog Post

   Published

   Sept. 8, 2015, 2:30 p.m.

   ...other plasmid-based systems, which will enable the assembly of larger systems. The work of Schmidl et al. ...

9. Resume Writing for Non-academic Science Careers

   Type

   Blog Post

   Published

   Dec. 18, 2014, 4:06 p.m.

   ...skills, and the job you are applying for. Also remember that a resume is typically no more than 2 pages...

10. Fluorescent Proteins 101: Luciferase

    Type

    Blog Post

    Published

    June 24, 2014, 3:59 p.m.

    ...as the most efficient means to disrupt the cell membrane and deliver the substrate; however, secreted luciferase...

11. Advice for Scientists Starting a Lab

    Type

    Blog Post

    Published

    April 29, 2014, 1:43 p.m.

    ...scientists to their labs. Further Reading & Resources EMBO Lab Leadership Courses HHMI Resources for Early ...

12. Viral Vectors 101: Viral Applications

    Type

    Blog Post

    Published

    April 13, 2023, 1:15 p.m.

    ...-transfect cell types ranging from primary to embryonic stem cells. Viral integration of a construct is...

13. Hot Plasmids - February 2022

    Type

    Blog Post

    Published

    Feb. 22, 2022, 2:15 p.m.

    ... yet low-pressure teaching opportunity for lab members or students new to protein expression and purification...

14. Sweating the Small Stuff: Details in the Lab

    Type

    Blog Post

    Published

    Feb. 8, 2022, 2:15 p.m.

    ...side to your right as you use them can help you remember what's been added and what hasn't. Developing ...

15. Antibodies 101: Isotypes

    Type

    Blog Post

    Published

    Oct. 19, 2021, 1:15 p.m.

    ...tears, saliva, mucus) where it protects mucosal membranes. This isotype is most commonly a dimer with a ...

16. Why and how to start studying chromatin (with Addgene's help)

    Type

    Blog Post

    Published

    June 12, 2018, 11:40 a.m.

    ...happens, there are a lot of scientists who not only remember to think about DNA in this context, but also specifically...

17. Improved Plasmid Maps Powered by SnapGene

    Type

    Blog Post

    Published

    June 29, 2017, 1:06 p.m.

    ...colleagues, or explain plasmid features to a lab member when you’re not at your computer. Maps, sequences...

18. Luminescent Imaging with Nano-lanterns

    Type

    Blog Post

    Published

    May 25, 2017, 2:30 p.m.

    ...publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns for...

19. Multiplex Genome Editing with CRISPR-Cpf1

    Type

    Blog Post

    Published

    May 9, 2017, 2:12 p.m.

    ...Disadvantages References Yamamoto Lab Golden Gate Assembly transfection One vector expresses Cas9 and ...

20. Screening for Successful Genome Editing with Digital PCR

    Type

    Blog Post

    Published

    March 30, 2017, 2:30 p.m.

    ... in addition to testing their performance (2). Remember that although digital PCR is a great screening...

21. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type

    Blog Post

    Published

    May 26, 2020, 1:15 p.m.

    ...specific locus to create a knock-out in human embryonic stem cells.  Find GEmCherry2 reporter plasmids...

22. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type

    Blog Post

    Published

    May 19, 2020, 1:15 p.m.

    ..., spike proteins, envelope small proteins, and membrane proteins. Although all viral components are available...

23. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type

    Blog Post

    Published

    Jan. 30, 2020, 2:15 p.m.

    ...conducting the experiment (note: registrations can be embargoed while the research is ongoing, but all registries...

24. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type

    Blog Post

    Published

    Nov. 5, 2019, 1:28 p.m.

    ...enzyme can help determine which enzyme to use. Remember, accessibility, specificity, and sensitivity are...

25. CRISPR 101: Non-Homologous End Joining

    Type

    Blog Post

    Published

    April 16, 2015, 3:45 p.m.

    ... works in Dale’s lab.        Dale Ramsden is a member of the curriculum in Genetics and Molecular Biology...

26. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type

    Blog Post

    Published

    Feb. 18, 2014, 4:22 p.m.

    ... multiple mentees efficiently Allows all group members to lead and learn at the same time Broadens the...

27. Addgene: The First Twenty Years

    Type

    Blog Post

    Published

    Dec. 19, 2024, 2:15 p.m.

    ...labhold” name. Our first plasmid request came on September 8, 2004, for pcDNA-p160MBP, while the first request...

28. Viral Vectors 101: An Introduction to AAV

    Type

    Blog Post

    Published

    May 30, 2023, 1:15 p.m.

    ...expression for several years (Wonjo et al., 2013). Remember that AAVs do not integrate into the host genome...

29. The time and cost required to make a plasmid

    Type

    Blog Post

    Published

    March 23, 2023, 1:15 p.m.

    ... is a concern. The faster methods, like Gibson assembly, are typically more expensive, so you’ll have ...

30. Summer SciComm: Preprints

    Type

    Blog Post

    Published

    Aug. 16, 2022, 1:15 p.m.

    ...journal and share it more widely!  Not everyone embraces preprints as part of their scientific communication...