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Showing: 391 - 420 of 701 results

  1. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...control for a specific marker in your experiment. To make an FMO sample, you take a subset of your experimental... is prepared very similarly to a FMO sample: you make a sample from a combined subset of your experimental...

  2. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...and molecular biologists use to tinker with DNA. Making changes to a DNA sequence can help scientists identify... are a few drawbacks with these approaches that makes it challenging for users to achieve targeted diversification...

  3. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...If you’re using CRISPR to make a genome edit, how do you know if your CRISPR experiment was successful...different constructs for the reporter to work and to make the desired genome edit:  A donor plasmid that ...

  4. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...of their optimally suited temperature ranges and make them grow better at suboptimal ones, which could...within a regular mesophilic host system. This would make the host able to grow at low temperatures suitable...

  5. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...then inserted into the host's genome and is used to make more virus. The most studied (and used in the lab...also transduce dividing and non-dividing cells, making it conducive to targeting all cell types. Altogether...

  6. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ... Thus, the adjustable nature of viral libraries makes them the ideal vector for the job. Therapeutic ...immunogenicity, and cell-specific targeting features make them an ideal delivery system for human patients...

  8. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...     The Trimmer Lab has continued to work to make the sequences of monoclonal antibodies publicly ...sequences from the Trimmer lab hybridoma collection and make them publicly available. The lab has also expanded...

  10. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...testing and a lack of well-characterized genetic tools make it challenging to engineer a specific function in...construct our color changing flower we are carefully making our way through the IP landscape, using plant parts...

  11. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...exposure to autophagy-inducing conditions. Rosella makes it easier to study the mechanisms behind autophagy... shown in Sargsyan et al. It is also possible to make Rosella fusions and thereby monitor autophagy of...

  12. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...However, they note that certain improvements could make CRISPRainbow even better. Adding an additional hairpin...activation/repression and genome editing. Ready to make your cells a little more colorful? CRISPRainbow ...

  14. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...promote tumor durability instead of repressing them, making it even harder to cure cancer. To understand how...Witz, Isaac P. "The tumor microenvironment: the making of a paradigm." Cancer Microenvironment 2.1 (2009...

  15. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...systemically administered in an organism. These features make AAV ideal for human therapies and in vivo laboratory...delivery of genetic cargo to a host cell. In order to make room for a gene of interest, AAVs used in research...

  16. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...suppression of expression is faster and easier than making a knockout and is a great way to temporarily reduce...integrated (once or many times) into the genome. Make sure to choose an appropriate promoter to express...

  17. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...developed by engineering the Cas12f scaffold gRNA and making strategic mutations in the DNA binding cavity of...half the size of the Cas9 or Cas12a based systems, making it well suited for AAV packaging. Most importantly...

  19. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...comment online! Want the general public (say, policymakers or community organizations) to use your work...those plasmids alongside a journal article, but it makes the research materials available to the community...

  20. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...time to read the book.  Intuitively, this probably makes sense. It’s a lot easier to read a formulaic fiction...happens to us all!) and offer a variety of options to make your content more accessible to your audience.  ...

  21. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...short linker peptide. The lack of constant region makes them less immunogenic, but they have lower affinity...produced in bacteria and are generally cheaper to make than antibodies. Minibodies are created by binding...

  25. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...safety profiles as well as other benefits. What makes AAV vectors great gene delivery tools? There are...homology directed repair (HDR). This characteristic makes AAV vectors especially amenable to correcting disease-associated...

  26. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...transfer office or other authorized office directly to make sure the deposit agreement/MTA is taken care of ...spreadsheet will help expedite your data entry and make it easier than ever to share your discoveries with...

  27. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ... of MoClo. Image courtesy of Rob Hurt. What makes MoClo systems so powerful is their modularity — ...engineering complex cellular behaviors requires making complex manipulations to cells. For example: in...

  28. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...to CRISPR experiments, therefore, you often must make a choice about what is your top priority: location...trying to knock-in a patient mutation or need to make a precise amino acid change, there is very little...

  29. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...receiver cells. There are over 40 trillion cells that make up the human body and G-baToN provides versibility...

  30. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...effectiveness, if the editing frequency isn’t high enough to make a physiological difference, then the treatment will...systems as well as improving delivery strategies to make fast, effective, and targeted therapy possible. ...
Showing: 391 - 420 of 701 results