...step of the series is consistent at 1:2. When preparing a dilution series, use a new pipette tip for each...components can affect the final readout. When preparing a standard curve, change pipette tips and mix ...

...community is a kind of duty for researchers when considering the peer-review system of journals and grant ...technologies developed abroad, so it's a little boring. I think that such tendency is one of the reasons...

...Neighborhood.   Work/life balance. Is it truly possible to bring all aspects of our lives under control? Balance ... friends. If there is any guilt, you should be sharing it absolutely equally, which means you only have...

...pinhead—contribute to the “sculpting” of tissues and organs during embryo development. Single cell sequencing-based...Single-cell reconstruction of developmental trajectories during zebrafish embryogenesis." Science 360.6392 (2018...

...about. Instead of reading a paper and getting my bearings, I would read a paper, panic, and then download...think the field is going or what areas are worth exploring.  Edit and rewrite, then repeat If you’re like...

...mesothelioma. Asbestos, and certain other naturally occurring asbestiform minerals such as erionite, are the...an immediate stop to the use of asbestos, thus ensuring that future generations will be protected from...

...repressing and activating expression of TCF4 while monitoring transcript and protein levels. Plasmids for multiplexed...donor infection (CRISPR-READI) enables genome engineering using HDR donors lengths up to 4.9 kb. Find ...

... target guanosine instead, perhaps analogous engineering of UNG, which excises uracil, could allow it ...considerably. And there’s so much more exciting protein engineering going on in the base editing field! From CRISPR-free...

...inducible promoters with different degrees of stringency and inducibility (Toh et al. 2023), and additional...across gram negative hosts. Biotechnology and Bioengineering, 106(2), 326–332. https://doi.org/10.1002/bit...

...blog post). Optimizing FLP FRT through smart engineering Different FLP versions – making FLP efficient...Frank et al. 2003. “A Directional Strategy for Monitoring Cre-Mediated Recombination at the Cellular Level...

...full-length (19-20 bp) or short (17-18 bp) guides. When comparing the toxicity of guides that have 1-bp mismatch...Central PMCID: PMC3988262. 4. Hart, Traver, et al. "Measuring error rates in genomic perturbation screens: gold...

...Vader in "Return of the Jedi" While Vader was referring to the evil side of a mystical "Force," this quote...posttranslational modifications can disrupt FP folding rendering the protein non-fluorescent. Unfortunately, accumulated...

...the plasmid without the independent variable. Referring back to the experiment associated with Figure ...aliquot/preparation. The replicate control isn’t measuring or assessing reproducibility of the effect of ...

...high protein levels including:  ompT: Strains harboring this mutation are deficient in outer membrane ...recommended as these auxillary plasmids may be isolated during the prepping process. How does inducible expression...

...Diversity and inclusion in science, technology, engineering and mathematics (STEM) (17). This effort is two-fold... “politicized science” ABC News: White House considering gutting 38 EPA programs: draft budget  Science...

...sequence detection method based on CRISPR-Cas12. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.02.29.971127... Detection of SARS-CoV-2 Genetic Material. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.04.20.052159...

...vivo probes, due to low autofluorescence, low scattering, and deep penetration of red-shifted light. The...about cellular events via  fluorescence changes occurring due to protein synthesis, degradation, or reconstitution...

...tribulations of the process, I’ve provided guidance and mentoring to several people that found my insights helpful...'s about how YOU will fit into a department and bring in grants. What kind of colleague will you be? Do...

...stain-free imaging systems are both effective for measuring the total protein present in a lane (Aldridge ...individual protein. This ensures you are truly comparing the abundance of your POI as a fraction of the...

...donor, you can deliver a silent mutation in a neighboring nucleotide along with your desired edit. This...Concordet J-P. Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection...

...editing tailored to smaller patient populations. To bring DMD CRISPR therapy closer to the clinic, much work... Mary Gearing...

...seems to be the case that there is no universal scoring system for selecting a gRNA, as the method of producing... is taken in the interpretation of results by requiring multiple sequences targeting a gene to score in...

...transferable skills while working in the lab and by offering advice on areas where you might need some help...Scientists: Managing v Leading Gain Management Skills Volunteering at a Professional Organization Resources on...

...tracing techniques: exploiting the offspring of genetic engineering." Journal of neuroscience methods ...

...looking for, but there are also benefits gained by lowering the complexity of the screen. For example, the..., Raj, et al. "Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach....

... most common method is transfection of short interfering RNA (siRNA). Knockdown caused by siRNA is also...cytometry experiment. But validation is critical to ensuring accurate interpretation of your results. Therefore...

...finding a good name for Blugene’s virus friend.  During the month of January, we’ll be taking name suggestions...

...revamped Data Hub! The community needs more data-sharing heroes like you.)  ...

...to be a helpful desktop reference. Each chapter brings together all of our 101 blog posts on an antibody-specific...

...the nervous system, and vectors for zebrafish engineering. You can find additional hot plasmids in our ...

...CRISPR campaign! But if you missed our tweets featuring the best of CRISPR in 2021 and useful resources...

... Although we're storing away all of our holiday decorations, our holiday spirit remains. We would like...

...navigate the scavenger hunt. Good luck and have fun exploring the blog!  ...

... post. Alongside our efforts to facilitate the sharing of reagents and data, we’re also publishing research...

... science comic not on our list? Let us know by sharing it with us in the comments.   ...

...vial filling, accurate tube-labeling, quick plate pouring, and much more. We didn’t want to keep the knowledge...

... text, we invite you to join us! We'll also be sharing our conference experience on X, Instagram, and ...

...editor of Asimov Press and is designing a genetic engineering curriculum at MIT. He also writes Codon, a blog...

...will be required. If you have any questions on ordering plasmids, please feel free to email [email protected]...

...questions about this or other Addgene shipping and ordering processes, don’t hesitate to contact our friendly...

...production protocol on our website and tips for titering your lentivirus in another blog post. If you need...

...Get Tips on Verifying Your Plasmid Table 1: Comparing Typical Comericial Ladders with the PSU Ladders...

... some useful career advice.  Our first video, featuring Outreach Scientist Jessica Welch, can be found...

...biophysics of protein folding and selective pressures during protein evolution. The Baker Lab has deposited ...

...University of Malawi where he focused on agricultural engineering through biotech. After graduating with a masters...

...you plan PEI transfections for AAV packaging, considering using this PEI Calculator. AAV packaging typically...

...December 31, 2021. Let us know which contest you are entering in (if you don’t include this, we will make our...

...different strains of E. coli,” says Westenberg. During the transformation experiment, he plans to show...

...  The CRISPR field moves fast. That’s why we’re bringing you a brand new third edition of Addgene’s CRISPR...

...suspect that our costume contests were indeed occurring earlier but that the photo evidence was destroyed...