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...continuation of our efforts to provide resources to researchers in developing countries or that work in under-funded...continued opportunity to get plasmids to the researchers who need them the most. Check back here to learn...more about future awardees and their inspiring research. Additional Resources on the Addgene Blog Learn...Resources Learn About Our Grants to Rare Disease Research Resources on Addgene.org Check out our Cancer...

... Mentor Learn How the Future of Research Looks to Improve the Research Process  Browse our Career Posts...Plasmids Find Plasmids for Your Research ... research project...

...important. Antibodies are a critical component of the researcher toolkit due to their ability to bind to molecular...culprits in the reproducibility crisis of biomedical research (Baker, 2015; Bradbury & Plükthorn, 2015).  To...which represent five experimental strategies researchers can use to show that their antibody performs ...Reproducibility: Standardize antibodies used in research. Nature 518:27–9. https://doi.org/10.1038/518027a...finding and validating suitable antibodies for research. Mabs 8:27–36. https://doi.org/10.1080/19420862.2015.1100787...

... and cloned by Neville Sanjana. This fantastic research tool has been requested from the repository nearly...over a year and a half, a small sampling of the research it has already been used for includes work probing...plasmids being distributed to and accelerating research in labs all around the world. We thank our many...depositors for making these reagents available to the research community through Addgene and look forward to ...

...You can also use the "Search Table" box at the upper right of the table to search and filter the list of...here to explore the TetO-FUW-OKSM plasmid page. Or search for your favorite plasmids and see where they've...the article that has used this plasmid. Happy researching! If you need any assistance finding plasmids ...

...fluorescent proteins like GFP came to be used as research tools is particularly interesting. Although humans...light and glows green. In the late 1980s, another researcher, Douglas Prasher, got the idea that this new ...of GFP, and began to realize its potential as a research tool. Soon, the number of people studying it grew...proteins with new colors Around the same time, researcher Sergey Lukyanov realized that, although they ...

...Permission to use materials for research or teaching purposes Rights to all research results, modifications,...understand - you’re a busy scientist working hard on research, and you need the right experimental materials... receiving institutions and their respective researchers. Check out the lists below for more details. ...

...Choosing a Research Project Finding and Joining Your Dream Lab Advice for Choosing a Research Project Advice... to build and lead a productive and successful research lab. Once goals are reached, how do science labs...day trip each year and as we do a lot of yeast research, a few of those have been trips to breweries who...

...goals of accelerating research and discovery by improving access to useful research materials and information...impact made possible by the sharing of tools for research: discovered and developed by our depositors, used...ways to support a global community where every researcher feels both welcomed and resourced for success...

...story of base transversion editors starts with researchers in the Keith Joung lab, who were engineering ...editing between bacteria and mammalian cells Researchers in the Xueli Zhang lab were making similar observations...develop more efficient and precise editors. Most researchers agreed on the first part of the likely editing... efficacy varied among target sequences. The researchers hypothesized that the CGBE edit process occurs...experimented with adding different proteins to the CGBE architecture. They conducted a CRISPRi screen to identify...

...changes in DA concentration. To address this, researchers from some labs have developed fluorescent-protein... tool to detect and image DA in live animals (Patriarchi et al., 2018). Similar to calcium and glutamate...PMID: 4403305. PubMed Central PMCID: PMC426888. Patriarchi, Tommaso, et al. "Ultrafast neuronal imaging ...protocols and videos Find biosensor AAVs for your research ...

...opsins) to manipulate cell activity, enables researchers to silence or incite neuronal firing and study...Identifying neurons that trigger spontaneous swimming  Researchers at the Isacoff lab applied optogenetics in zebrafish... distinguish between swimming behaviors, the researchers measured the tail movements of 5 day old fish... viral service Find optogenetics AAVs for your research Find all AAVs from the viral service ...

...collaborative endeavor providing plasmids to researchers in developing countries. We are proud to introduce...this important agricultural plant. Her current research focuses on endophytes, bacteria, and fungi that...applying gene technology to bacteria and plants. Her research involves cloning cry genes from the soil bacterium...groups of scientists working in a wide range of research fields and geographical locations. We are excited...

...them spread, but also stresses the host. The researchers suspected natural evolution would produce transposons... by directed evolution using bacteriophages. Researchers moved a gene required for phage propagation to...phages to outcompete their brethren, and the researchers isolated these more efficient CASTs (Witte et...step toward that kind of gene treatment. The researchers also compared evoCAST to the Liu lab’s own PASSIGE...

...conference. It was inspiring to see how microbiology research is improving lives worldwide and the presentation...the booth. We were happy to chat with lots of researchers who weren’t aware of Addgene’s plasmid sharing... plasmid tools on the horizon for microbiology research and we’d love to help more microbiologists share...being the Director of the Great Lakes Bioenergy Research Center and a fellow Rhodobacter enthusiast, Tim...

...own and only share what they think will get the research funded,” she says. Baradaran met Addgene Executive...reagents such as plasmids and viral stocks for researchers around the world who would otherwise have difficulty...Discovery at the Novartis Institutes for Biomedical Research. Drug development was something Stegmeier has ...got incredibly compelled by wet bench molecular research, but also became passionate about virology,” he...

...H134R)-EYFP   Control AAV Control AAV allow researchers to not only interpret their results, but also...to achieve different patterns of expression, researchers often need to determine these transduction patterns...sensors: Dopamine and norepinephrine Neuroscience research relies on accurate monitoring of neurotransmitters... viral protocols and videos Find AAVs for your research ...

...partnership with Allele Biotechnology which allows researchers to deposit plasmids containing the fluorescent...rather than cyan and named the protein mTFP1. The researchers recommend using mTFP1 with a 445/30 nm excitation...function of the protein that they are fused to. The researchers constructed 22 different mTFP1 fusions to both...posts about choosing fluorescent proteins for your research Subscribe to the Addgene blog Resources on Addgene.org...

...genome and proteins. This is, for the intrepid researcher looking for molecular biology tools, a very useful...replication When it comes to replication, viruses tend to march to the beat of their own drum. They have a wide ... eliminate or reduce the risk of infection — researchers were able to create far safer viral vectors that... The four types of viral vectors (which many researchers will informally refer to as viruses) are named...

...visibly complex - and unique - adult. My initial research directions followed that avenue with projects ...interests and skills than what fits for applied research. I focus in general terms on a couple of very ...teaching – often explaining a technical piece of research to others outside that specialty, who may be other...professional meetings, societies, networks just as do research scientists. There we meet others in the scientific...