We narrowed to 582 results for: BASE

... more than one person, we selected the finalist based on the first time of submission.)     We love...

...predicted deleteriousness of the amino acid change based on maps of protein structure. Sometimes this provides... years, we’ve hoped that large scale laboratory-based functional experiments that classify which variants...

...modified) for optimal stability and efficacy.    Base modifications What is more stable than RNA? DNA!...gRNAs for Spatiotemporally Controlled CRSIPR-Cas-based Genomic Regulation. ACS 6, 5, 695-703 (2020). https...

...critiques or endorsements. In contrast, journal-based peer review is usually a confidential process among...ensure a manuscript’s quality. But even journal-based peer review can be prone to biases or errors, with...

...staining. Isotype controls are used in antibody-based applications including flow cytometry, immunohistochemistry...of two. There are four IgG subclasses, numbered based on their abundance in blood: IgG1, IgG2, IgG3, and...

...by gel or by nanodrop to assemble the  fragments based on their molar ratio. Assembly of the fragments ...you can assemble the different parts of a plasmid based on the cloning strategy you followed. While most...

...Addgene depositor Alice Ting used yeast display-based directed evolution to create APEX2. They have now...without special equipment. What’s more, detection is based on color changes to the assay paper, so no special...

...all be ssDNA within the viral particle. The ITRs base pair with each other to allow DNA synthesis of the...57 human adenovirus types, but most vectors are based on adenovirus 5 (Ad5).     Fig. 3 - Viral...

...mechanism for increasingly popular nucleic acid-based vaccines. These vectors provide several advantages...delivery system for human patients. Multiple viral-based gene therapies have already been approved by the...

...hours of hands-on time to make a single plasmid, based on the assumptions outlined in Table 1.   Of course...performing the experiments. This cost varies hugely based on a number of factors, but we can assume it’s at...

...that covers most things CRISPR - from nickases to base editing to pooled library screening. It also has...

...Open Hardware? Author: Benedict Diederich Course-Based Undergraduate Research Experiences (CUREs)Author...AccessibleResearcher: Ryan Mehl; Andy KarplusIBEX Knowledgebase: A Data Resource for Multiplex Tissue ImagingResearchers...YanevCellosaurus: A Cell Line Information DatabaseResearcher: Amos BairochViral Vectors 101: Systemic ...

...causes of irreproducibility with some solutions based on what I’ve learned. I hope you can apply some ...all human extracellular proteins with community-based validation. They also have plans to expand to other...with larger and larger datasets and use computer based analysis techniques, an important component of reproducibility...on the Addgene Blog FPbase: A new community-editable fluorescent protein database ReFigure: Save Scientific...technologies later. Organizations such as BenchSci and FPbase are working hard to enable researchers by making...BenchSci collates information about antibodies while FPbase curates information about fluorescent proteins ...

...TAG. Design your repair template with 55-100 base homology arms upstream and downstream of the insertion...using the ivTRT method, try to make the first two bases of the left homology arm guanine (i.e. GG); this... PMC3961003  Miura, Hiromi, et al. “CRISPR/Cas9-based generation of knockdown mice by intronic insertion...

...References Abril, U.N.O., et. al. (2022) A plasmid-based system for locating and containing escaped flies...

...the 3rd day of CRISPR, Addgene gave to me: dCas9-based gene editing for cleavage-free genomic knock-in ...

... broadens the conversation about genetics pgEd, based at the Department of Genetics of Harvard Medical...universities or cities have organizations (often volunteer-based) that put on public lectures, science cafés or science...

... delivery systems, especially lentiviral vector-based CRISPR pooled libraries that allow functional screening...barcoding library consists of 96 retroviral plasmids (based on the Murine Stem Cell Virus, MSCV) each with a...

...the Rare Genomics Institute by providing plasmid-based tools for rare disease research. Congratulations...events, we will utilize BRET-, FRET-, and intensity-based signaling biosensors to monitor intracellular signaling...

...protein for live cell imaging would be mTurquoise2, based on the data acquired at low excitation powers. Note...Amsterdam) where she develops genetically encoded FRET based biosensors.     Additional Resources on the Addgene...