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We narrowed to 574 results for: BASE;

Showing: 261 - 280 of 574 results

  1. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...staining. Isotype controls are used in antibody-based applications including flow cytometry, immunohistochemistry...of two. There are four IgG subclasses, numbered based on their abundance in blood: IgG1, IgG2, IgG3, and...

  2. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...all be ssDNA within the viral particle. The ITRs base pair with each other to allow DNA synthesis of the...57 human adenovirus types, but most vectors are based on adenovirus 5 (Ad5).     Fig. 3 - Viral...

  3. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...mechanism for increasingly popular nucleic acid-based vaccines. These vectors provide several advantages...delivery system for human patients. Multiple viral-based gene therapies have already been approved by the...

  4. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...hours of hands-on time to make a single plasmid, based on the assumptions outlined in Table 1.   Of course...performing the experiments. This cost varies hugely based on a number of factors, but we can assume it’s at...

  5. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...that covers most things CRISPR - from nickases to base editing to pooled library screening. It also has...

  6. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    Published
    Dec. 5, 2023, 2:15 p.m.
    ...Open Hardware? Author: Benedict Diederich Course-Based Undergraduate Research Experiences (CUREs)Author...AccessibleResearcher: Ryan Mehl; Andy KarplusIBEX Knowledgebase: A Data Resource for Multiplex Tissue ImagingResearchers...YanevCellosaurus: A Cell Line Information DatabaseResearcher: Amos BairochViral Vectors 101: Systemic ...

  7. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...causes of irreproducibility with some solutions based on what I’ve learned. I hope you can apply some ...all human extracellular proteins with community-based validation. They also have plans to expand to other...with larger and larger datasets and use computer based analysis techniques, an important component of reproducibility...on the Addgene Blog FPbase: A new community-editable fluorescent protein database ReFigure: Save Scientific...technologies later. Organizations such as BenchSci and FPbase are working hard to enable researchers by making...BenchSci collates information about antibodies while FPbase curates information about fluorescent proteins ...

  8. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...TAG. Design your repair template with 55-100 base homology arms upstream and downstream of the insertion...using the ivTRT method, try to make the first two bases of the left homology arm guanine (i.e. GG); this... PMC3961003  Miura, Hiromi, et al. “CRISPR/Cas9-based generation of knockdown mice by intronic insertion...

  9. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...References Abril, U.N.O., et. al. (2022) A plasmid-based system for locating and containing escaped flies...

  10. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ...the 3rd day of CRISPR, Addgene gave to me: dCas9-based gene editing for cleavage-free genomic knock-in ...

  11. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...protein for live cell imaging would be mTurquoise2, based on the data acquired at low excitation powers. Note...Amsterdam) where she develops genetically encoded FRET based biosensors.     Additional Resources on the Addgene...

  12. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ... on the identification of autophagic structures based on morphology. Autophagosomes are relatively easy...summary of what color fluorescence Rosella emits based on its localization. Table 1: Summary of Rosella...

  13. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...and sensitive enough to detect gene copy number based on the number of fluorescent puncta observed. By...Addgene Blog Read More about Fluorescent Protein-Based Tools Learn Some of the Pitfalls When Using Fluorescent...

  14. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ... broadens the conversation about genetics pgEd, based at the Department of Genetics of Harvard Medical...universities or cities have organizations (often volunteer-based) that put on public lectures, science cafés or science...

  15. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ... delivery systems, especially lentiviral vector-based CRISPR pooled libraries that allow functional screening...barcoding library consists of 96 retroviral plasmids (based on the Murine Stem Cell Virus, MSCV) each with a...

  16. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...learn how to communicate their work differently based on their audience. How you talk about your experiments...problem was the creation of an Addgene lexicon. The base lexicon was and continues to be a list of commonly...

  17. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...the Rare Genomics Institute by providing plasmid-based tools for rare disease research. Congratulations...events, we will utilize BRET-, FRET-, and intensity-based signaling biosensors to monitor intracellular signaling...

  18. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ... modified) for optimal stability and efficacy.  Base modifications What is more stable than RNA? DNA!...gRNAs for Spatiotemporally Controlled CRSIPR-Cas-based Genomic Regulation. ACS 6, 5, 695-703 (2020). https...

  19. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...the concentration of proteins in an ELISA well is based on a colorimetric reaction. Most commonly, this ...concentration of protein in the experimental wells based on the equation of the line. With these tips in ...

  20. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...track cell-cell interactions By Jennifer Tsang GFP-based Touching Nexus (G-baToN) is a new tool developed... quantification of this depletion using imaging-based measurements (Ashley et al., 2021).   Schematic...
Showing: 261 - 280 of 574 results