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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 329 results for: BRE

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Showing: 261 - 280 of 329 results

  1. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...effectors. Nucleases generate a classic double-stranded break, while modulators regulate transcription and chimeric...

  2. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ... Figure 5: Mechanism of ATLASCre. VAMP2 = synaptobrevin 2; At = ALFA-tag; BACE = β-secretase; AF = AMPA.FingR...

  3. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...During DNA repair, there is a chance that the two breakpoints will repair without the section between the two...

  4. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...patience, as you wait for the anesthetized mice to breathe in the liquid. Restraint: anesthesia Location: ...

  7. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...-type Cas9 and a gRNA generates a double-strand break in the target DNA, which, when repaired through ...

  8. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ... the human ubiquitin C promoter. Anatomical abbreviations: Gr= Gracile tract, Cu= Cuneate tract, dCST=...

  10. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...CFP), and yellow FP (YFP) were derived. For a breakdown of GFP, it's variants, and their relevant mutations...

  11. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...method for you! And even if you’re not ready to break-up with your DNA purification kit, you can still...

  13. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    .... Jean Bennett (UPenn) that resulted in the groundbreaking 2001 study showing that AAV-mediated delivery...

  16. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...SRM), a family of imaging techniques developed to break through the resolution barrier while still utilizing...

  19. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...is sufficient. Controls: Controls can make or break an experiment. The best controls for a colony PCR...
Showing: 261 - 280 of 329 results