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...) everything ourselves and it’s a hard habit to break. But as a manager you will be quickly overwhelmed...

...binding the protospacer. The resulting double strand break (DSB) stimulates DNA repair mechanisms that can ...

.... However, if the molecules are confined to a subregion of the cell and/or they cannot tumble in 720°,...

...to get recharged. The fun change of pace helped break up the negativity and allowed me to focus on the...

...-type Cas9 and a gRNA generates a double-strand break in the target DNA, which, when repaired through ...

... the human ubiquitin C promoter. Anatomical abbreviations: Gr= Gracile tract, Cu= Cuneate tract, dCST=...

...small victory in a long road to your scientific breakthrough. More Reading: How to manage scientific data...

...CFP), and yellow FP (YFP) were derived. For a breakdown of GFP, it's variants, and their relevant mutations...

...of ~1.8 and an A260/230 of ~2.0. However, let’s break down how different contaminants impact these ratios...

...tools in molecular biology. “Retroviruses” is an umbrella term which encompasses 1.) the gamma-retroviruses...

...and the quality of a standard curve can make or break an experiment. Here, we will provide an overview...

...SRM), a family of imaging techniques developed to break through the resolution barrier while still utilizing...

...method for you! And even if you’re not ready to break-up with your DNA purification kit, you can still...

...Zhen, et al. "Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes...

.... Jean Bennett (UPenn) that resulted in the groundbreaking 2001 study showing that AAV-mediated delivery...

...employer. Your heart is pounding, you’re short of breath, and you’re a little jumpy. You are at another ...

...is sufficient. Controls: Controls can make or break an experiment. The best controls for a colony PCR...

...Science. 1986; 231:699-704. PubMed PMID: 3080805. 3. Brent, R., and Ptashne, M. A eukaryotic transcriptional...

...facility). The third tube is used to fulfill orders. Breaking the (bar)code At each step in the receiving, transforming...

...studies, act as a reporter for DNA double strand break repair, and to label pathogenic intracellular microbes...