We narrowed to 322 results for: BRE
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TypeBlog PostPublishedApril 18, 2024, 1:15 p.m....effectors. Nucleases generate a classic double-stranded break, while modulators regulate transcription and chimeric...
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Genome-wide Screening Using CRISPR
TypeBlog PostPublishedAug. 18, 2015, 2:30 p.m....-type Cas9 and a gRNA generates a double-strand break in the target DNA, which, when repaired through ... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m.... the human ubiquitin C promoter. Anatomical abbreviations: Gr= Gracile tract, Cu= Cuneate tract, dCST=... -
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog PostPublishedSept. 23, 2014, 3:05 p.m....small victory in a long road to your scientific breakthrough. More Reading: How to manage scientific data... -
Which Fluorescent Protein Should I Use?
TypeBlog PostPublishedMay 20, 2014, 2:06 p.m....CFP), and yellow FP (YFP) were derived. For a breakdown of GFP, it's variants, and their relevant mutations... -
DNA Purification Without a Kit
TypeBlog PostPublishedFeb. 11, 2020, 2:15 p.m....method for you! And even if you’re not ready to break-up with your DNA purification kit, you can still... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog PostPublishedSept. 6, 2018, 12:02 p.m....Zhen, et al. "Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes... -
AAVs in Retinal Gene Therapy
TypeBlog PostPublishedJuly 31, 2018, 12:55 p.m..... Jean Bennett (UPenn) that resulted in the groundbreaking 2001 study showing that AAV-mediated delivery... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog PostPublishedJan. 11, 2018, 3:29 p.m....employer. Your heart is pounding, you’re short of breath, and you’re a little jumpy. You are at another ... -
Plasmids 101: Colony PCR
TypeBlog PostPublishedMay 12, 2016, 2:30 p.m....is sufficient. Controls: Controls can make or break an experiment. The best controls for a colony PCR... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog PostPublishedJuly 27, 2023, 1:15 p.m....tools in molecular biology. “Retroviruses” is an umbrella term which encompasses 1.) the gamma-retroviruses... -
Getting to Know Expansion Microscopy
TypeBlog PostPublishedJune 20, 2023, 1:15 p.m....SRM), a family of imaging techniques developed to break through the resolution barrier while still utilizing... -
What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog PostPublishedJuly 1, 2025, 1:15 p.m....of ~1.8 and an A260/230 of ~2.0. However, let’s break down how different contaminants impact these ratios... -
Great Results Start with Great Standard Curves
TypeBlog PostPublishedAug. 15, 2024, 1:15 p.m....and the quality of a standard curve can make or break an experiment. Here, we will provide an overview... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog PostPublishedOct. 22, 2015, 2:30 p.m....Science. 1986; 231:699-704. PubMed PMID: 3080805. 3. Brent, R., and Ptashne, M. A eukaryotic transcriptional... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog PostPublishedOct. 6, 2015, 2:30 p.m....facility). The third tube is used to fulfill orders. Breaking the (bar)code At each step in the receiving, transforming... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog PostPublishedMay 15, 2014, 3:33 p.m....studies, act as a reporter for DNA double strand break repair, and to label pathogenic intracellular microbes... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog PostPublishedApril 23, 2014, 1:08 p.m.... insert encoded by the vector." The table below breaks down the biosafety concerns associated with various... -
Negotiating Work and Life: How to Find the Joy
TypeBlog PostPublishedMarch 20, 2014, 3:04 p.m....exception is that men cannot actually give birth and breastfeed. Keeping this always at the forefront will go... -
Antibiotic Resistance: An Old Solution but a New Problem
TypeBlog PostPublishedNov. 10, 2016, 3:30 p.m...., we can all work together to try and prevent outbreaks of drug resistant bacteria within communities,...