We narrowed to 303 results for: GAI

1. Changing Labor Laws Bring Increased Postdoc Wages

   Type

   Blog Post

   Published

   Oct. 25, 2016, 2:30 p.m.

   ...as of October 1st the salary minimum was raised again to $51,600, itself a raise of 3.2%. Other institutions...

2. CRISPR Activation: A Practical Guide

   Type

   Blog Post

   Published

   Aug. 18, 2016, 2:30 p.m.

   ... large scale CRISPR-based screens can be used to gain insight into protein structure and function (Pan...

3. The Challenges of Cell Culture

   Type

   Blog Post

   Published

   May 5, 2016, 2:30 p.m.

   ...He studied biochemistry at Warwick University, gaining extensive cell culture research experience through...

4. Plasmids 101: The Promoter Region – Let's Go!

   Type

   Blog Post

   Published

   April 3, 2014, 8:05 p.m.

   ...each responsible for a specific subset of RNAs. To gain this specificity, the eukaryotic RNAP can recognize...

5. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

   Type

   Blog Post

   Published

   April 9, 2019, 1:13 p.m.

   ...PMID: 29505611. PubMed Central PMCID: PMC5837189. Nagai, Takeharu, et al. "Expanded dynamic range of fluorescent...

6. Hot Biosensors 2022: Year-End Roundup

   Type

   Blog Post

   Published

   Dec. 6, 2022, 2:15 p.m.

   ...kinetics and high signal/noise ratio. Here, Takeharu Nagai and colleagues present an improved genetically encoded...

7. Transferable Skills Guide: Conflict Resolution

   Type

   Blog Post

   Published

   Jan. 4, 2022, 2:15 p.m.

   ...accomplish, but there is a potential for long-term gains when you can do it. If Party X proposes solution...

8. Antibodies 101: Normalization and Loading Controls for Western Blots

   Type

   Blog Post

   Published

   April 8, 2025, 1:15 p.m.

   ...Ah, the notorious western blot: we meet again. So useful, yet so finicky to design and optimize. Today...

9. Filming Science Videos in the Age of Social Distancing

   Type

   Blog Post

   Published

   May 20, 2021, 1:15 p.m.

   ...what works and what doesn’t, and trying all over again. Here’s that process!   Figure 1: The Design-Build-Test-Learn...

10. Tips for Getting a Faculty Position

    Type

    Blog Post

    Published

    July 20, 2023, 1:15 p.m.

    ...create a new field, impact disease treatment, etc., again in clear terms. "Implications for human disease"...

11. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type

    Blog Post

    Published

    April 4, 2024, 1:15 p.m.

    ... populations with basically a smear in between. Again, it can depend on the type of stain whether a tighter...

12. An Integrin Antibody Toolkit from IPI

    Type

    Blog Post

    Published

    March 28, 2024, 1:15 p.m.

    ...αVβ3.13, may also block function of mouse αVβ3, again the first known reagent to do so. The 15 hybridoma-derived...

13. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type

    Blog Post

    Published

    Sept. 5, 2017, 1:58 p.m.

    ...template that corrects the blocking mutation and again identify the right clone from a few hundred. From...

14. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type

    Blog Post

    Published

    Feb. 22, 2017, 3:30 p.m.

    ...in the same orientation as those in the plasmid. Again, if they are in the opposite direction, both strands...

15. Evolution of Lab Techniques

    Type

    Blog Post

    Published

    June 21, 2016, 2:30 p.m.

    ... map DNA and examine genomes (6). The knowledge gained through these experiments allowed researchers to...

16. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type

    Blog Post

    Published

    March 1, 2016, 3:30 p.m.

    ...options in the deposit process in 2014 because of its gain in popularity. Gibson assembly is well known for...

17. How to Design Your gRNA for CRISPR Genome Editing

    Type

    Blog Post

    Published

    Sept. 24, 2020, 1:15 p.m.

    ...signal in these assays (Dempster et al., 2019). Again, the experimental strategy is clear: for any gene...

18. Hot Plasmids: Spring 2025

    Type

    Blog Post

    Published

    June 3, 2025, 1:15 p.m.

    ... set of highly-specific recombinant antibodies against three of the six human glypican proteins (GPC1,...

19. Antibodies 101: The Basics of Western Blotting

    Type

    Blog Post

    Published

    March 8, 2021, 2:41 p.m.

    ...blocked, it is stained with a primary antibody against your protein of interest. But which antibody should...

20. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type

    Blog Post

    Published

    May 18, 2018, 7:32 p.m.

    ...directly by creating well characterised antibodies against all human extracellular proteins with community-based...