We narrowed to 378 results for: Hal
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TypeBlog PostPublishedJune 30, 2016, 2:30 p.m....plasmids. It turns out that restriction enzymes are one half of naturally occurring restriction modification ...
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Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog PostPublishedJune 2, 2015, 6:06 p.m....encoded only once, and each <200 bp tRNA-gRNA takes up half the space of a standard pol III-gRNA, which will... -
CRISPR 101: Non-Homologous End Joining
TypeBlog PostPublishedApril 16, 2015, 3:45 p.m....Strande NT, Pryor JM, Strom CN, Mieczkowski P, Burkhalter MD, Oh S, Qaqish BF, Moore DT, Hendrickson EA... -
Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur
TypeBlog PostPublishedJuly 10, 2014, 7:17 p.m....his co-founders raised $6 million. The biggest challenge during this time was learning to communicate effectively... -
Will You Be My Mentor? Finding and Asking for Mentoring Support
TypeBlog PostPublishedFeb. 4, 2014, 3:50 p.m....current position Understand your interpersonal challenges if they know some of the people you work with... -
RaPID Detection of RNA-protein Interactions
TypeBlog PostPublishedAug. 16, 2018, 12:40 p.m....bind an RNA motif of interest in living cells. Challenges with existing RNA-protein interaction methods... -
dTAG - You're it!
TypeBlog PostPublishedJune 21, 2018, 2:06 p.m....molecules by conjugating a CRBN-binding ligand (thalidomide) to an FKBP12F36V binding ligand (ortho-AP1867... -
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute
TypeBlog PostPublishedFeb. 2, 2018, 3:20 p.m....finding that uninterrupted time to be creative is challenging,” says Susan. Another difficulty is that some... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog PostPublishedSept. 8, 2020, 2 p.m.... Empower and Accelerate Research Discoveries) Challenge provides grants for rare disease research. This... -
RNA Extraction Without A Kit
TypeBlog PostPublishedMarch 24, 2020, 1:15 p.m....until homogenization and cell lysis. This can be challenging depending on your laboratory situation or tissue... -
Human Germline Editing Using CRISPR
TypeBlog PostPublishedAug. 10, 2017, 2:19 p.m....heterozygous mutations, it will clearly be much more challenging for homozygous mutations. Moreover, introducing... -
Summer SciComm: Preprints
TypeBlog PostPublishedAug. 16, 2022, 1:15 p.m....Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla I (2022) A guide to preprinting for... -
Antibodies 101: Affinity Reagents
TypeBlog PostPublishedApril 14, 2022, 1:15 p.m....reduced-in-size options often have reduced binding strength, half-lives, and/or specificity as well. They’re more ... -
Antibodies 101: Secondary Antibodies
TypeBlog PostPublishedDec. 21, 2021, 2:15 p.m....protein of interest is often one of the most challenging components of any immunoimaging assay. Secondary... -
What's New in CRISPR - November 2021
TypeBlog PostPublishedNov. 18, 2021, 2:15 p.m....cavity of Cas12f. The CasMINI system is less than half the size of the Cas9 or Cas12a based systems, making... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog PostPublishedMay 11, 2021, 1:15 p.m.... M, Mueller SAL, Roseboom PH, Oler JA, Kalin NH, Hall RA, Smith Y (2019) Ultrastructural localization ... -
Viral Vectors 101: An Introduction to AAV
TypeBlog PostPublishedMay 30, 2023, 1:15 p.m....strand synthesis. In contrast, a scAAV vector with half the size of the ssAAV genome has a mutation in the... -
Transferable Skills Guide: Public Speaking
TypeBlog PostPublishedApril 20, 2023, 4 p.m....Technical difficulties turn your slide deck into a chalk talk. Your translator doesn’t know the term “diving... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog PostPublishedOct. 29, 2019, 2:52 p.m.... wild type (Oveido et al., 2010). One of the challenges of studying bioelectricity is that it can be ... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog PostPublishedApril 22, 2015, 2:06 p.m.... with Cas9 guided by different promoters. The challenge in building genetic circuits is getting enough...