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We narrowed to 712 results for: Met

Showing: 261 - 280 of 712 results

  1. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    ...breakthrough in cloning technology with the Golden Gate method. For the first time, it was possible to quickly...tedious and time-consuming traditional cloning methods, Lohmann said. The key component of Golden Gate...their lab learned the hard way after the cloning method, which had been humming along at 99 percent efficiency... et al. "A One Pot, One Step, Precision Cloning Method with High Throughput Capability." PLoS ONE. 3(11...

  2. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ...In complex metazoans, rapid cell division and large scale cell mobility are essential processes during...specificity or from the thymus to the lymph nodes. These methods gave researchers much more information regarding...allows imaging of living tissue up to about one millimeter in depth. Two low-energy photons (typically from... between several different immune cells. These methods allowed immunologists to develop a spatiotemporal...absorption properties of fluorescent proteins." Nature methods 8.5 (2011): 393-399. PubMed PMID: 21527931. PubMed...

  3. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nature Methods, 14(10), ...technically cell-permeable, its use in live cells can sometimes result in cellular toxicity due to the permeation...to small-molecule fluorescent labels. In Nature Methods (Vol. 19, Issue 2, pp. 149–158). Nature Research..., 987–994. https://doi.org/10.1038/nmeth.4403 Hayashi-Takanaka, Y., Stasevich, T. J., Kurumizaka, H., ...

  4. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...common method for checking both quantity and quality of your plasmid prep is by spectrophotometry. Achieving...one you are most familiar with. And, hey — if something is working for you, then there is no reason to...  Both bead- and column-based DNA purification methods perform well in terms of quality and yield. Bead-based...purification kit options Manufacturer DNA Isolation Method Kit Sizes Available Standard Miniprep Cost Reagents...

  5. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...sense dopamine. Methods for measuring Dopamine In vivo microdialysis The gold standard method of measuring... liquid for quantitation. The advantage of this method is that it can be performed in a live awake animal...the probe into a specific brain region. While the method is powerful and offers immediate measurements in...

  6. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...analysis in Drosophila. Nature Methods 9:47–55 . https://doi.org/10.1038/nmeth.1800 Isaacman-Beck J, Paik ...Maimon G, Wilson RI, Clandinin TR (2019) SPARC: a method to genetically manipulate precise proportions of...-wide reconstruction of single neurons. Nature Methods 15:1033–1036 . https://doi.org/10.1038/s41592-018...

  7. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...hormones strigolactones By Fabienne Furt Current methods used to detect strigolactones (SLs) are costly,...that of the internal control (Fig 1). Because this method relies on the fluorescence ratio between the 2 ...approach was also used to build and validate a ratiometric fluorescent biosensor based on the parasitic ...CRISPR library from Alec Kimmelman targets mouse metabolic genes with ~6 gRNAs per gene.  CRISPR-Act3.0, ...

  8. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ...world of academia often emphasizes structured methodologies and rigorous research plans. However, amidst...in nature or visiting museums can be excellent methods to detach from the constant stream of thoughts ...PhD studies, where every aspect of research is meticulously planned, it's easy to get stuck in your everyday...of postdocs focusing on molecular biology and metabolism. He now guides fellow PhDs in their careers with...

  9. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    Published
    Jan. 21, 2020, 2:30 p.m.
    ...relevant metadata and the screen results. Screenshots updated 2024. Importantly, metadata for each...to screen result interpretation. The analytical method for score assignment and significance thresholds...gene hits for each screen are also captured as metadata.   Screen results are reported as the original...

  10. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ...that was developed by Li, et al., in 2011. This method is ligation independent, it does not require purification...and then combine the products and digest with methylation sensitive restriction enzyme DpnI to remove the... Note—templates must have been isolated from methylation competent E. coli for this procedure to work ..., sequence-and ligation-independent PCR cloning method." BMC biotechnology 11.1 (2011): 1. PubMed PMID...

  11. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ... substrates inside the lysosome. Historically, methods for studying autophagy focused on counting the ...Daniel J., Ana Maria Cuervo, and Per O. Seglen. "Methods for monitoring autophagy from yeast to human." ..., Noboru, Tamotsu Yoshimori, and Beth Levine. "Methods in mammalian autophagy research." Cell 140.3 (2010...quantitative Pho8Δ60 assay of nonspecific autophagy." Methods in enzymology 451 (2008): 33-42. PubMed PMID: 19185711...

  12. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...suitable for flow cytometry and fluorescence microscopy, they also show that this method is compatible with...University has developed a new CRISPR screening method that makes use of guide RNAs with internal barcodes...the majority of cells receive one gRNA. The iBAR method resulted in high quality data even when used at...

  13. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ... or good - they're just exist and sometimes we benefit and sometimes we don't."     - Jennifer Tsang, ...age) fight in the Napoleonic Wars and against something even more sinister. 1000 pages long. Incredibly...International Finance. A book club could lead to smart, sometimes contentious, discussions. I presented the first...

  14. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type
    Blog Post
    Published
    June 4, 2019, 1:02 p.m.
    ... a high throughput manner. She helped develop a method that measures the effects of sequence variants ... the body’s response to warfarin. “These novel methods will be instrumental in interpreting human variation...sensor can also kinetically outperform current methods. Unger plans to use the sensor to answer the question...Pantoea agglomerans interaction. But the shape and metabolism of leaves can be changed by light and temperature...

  15. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...through our hot plasmids articles. Think we missed something smokin'? You can pitch a Hot Plasmids section ...of our most commonly requested antibodies. But sometimes, one antibody per target just isn’t enough and...agents. Adapted from Fig. 2 Campbell et al, Nature Methods   Find YFP plasmids here!   Campbell, B.C.,...fluorescent proteins for advanced microscopy. Nat Methods 19, 1612–1621 (2022). https://doi.org/10.1038/s41592...

  16. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ...your tables and figures. Now it's time to write. Sometimes, it's hard to get those words out and onto the..., you can take a longer 15-30 minute break. The method is named for the tomato or “Pomodoro” kitchen timer...manuscript they might be found, i.e. introduction, methods, results; or phrases are categorized by the goal...This podcast talks more about using the Pomodoro method in grad school Additional resources on the Addgene...

  17. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...taken up the plasmid in the same way. Selection methods, described below, are typically employed when creating...direct test for your gene of interest may be another method to assess transfection success. GFP is often used... a problem depending on the duration or other parameters of your experiment. Mammalian cells double at..., but still want to use transient transfection methods. Since there are no "natural" mammalian ORIs, scientists...

  18. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    Published
    July 6, 2017, 1:59 p.m.
    ..., starvation or the use of caloric restriction mimetics trigger autophagy and, in a mouse model led to...anti-cancer immunosurveillance and reduced tumor mass. Methods to study autophagy can be found here. Fun fact:...immunotherapy is the combination of the CRISPR/Cas9 methodology with T cell receptor engineering (Ref 5)  as ...Pietrocola, Federico, et al. "Caloric restriction mimetics enhance anticancer immunosurveillance." Cancer...

  19. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...explained in more detail here. There are a variety of methods that can be used to measure your fluorescent protein...complicating the interpretation. Because of this, these methods only give a qualitative view of dimerization tendency...the protein forms dimers. Finally, spectroscopic methods (based on fluorescence polarization) can be used...homodimerization. However, all the aforementioned methods only determine the capacity of homodimerization...

  20. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ... remind you of anything? Perhaps the Scientific Method? Imagine your problem is just a question you have... skills” is the ability to apply the scientific method to problems in a variety of contexts.  How do you...skills? Thankfully, as scientists, the scientific method is a core part of our academic training.  Developing...their logic. Or you can try applying the scientific method to everyday problems in your life. This tactic ...
Showing: 261 - 280 of 712 results