We narrowed to 459 results for: SHI
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TypeBlog PostPublishedApril 25, 2017, 2:30 p.m....Dronpa protein (named after dron, a ninja term for vanishing, and pa, for photoactivation) (6). Dronpa is photoactivated...PMID:16550175. 6.Ando, Ryoko, Hideaki Mizuno, and Atsushi Miyawaki. "Regulated fast nucleocytoplasmic shuttling...
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Antibodies 101: Flow Cytometry
TypeBlog PostPublishedJuly 20, 2021, 1:15 p.m....channels. In a typical assay a user will compare the shifting intensity of events. Since intensity positively...positively correlates with expression, the further the shift of a cell from the negative control, the higher ... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog PostPublishedMay 28, 2024, 1:45 p.m....inside the cells and are thus typically used for distinguishing live from dead cells. The combination of antibody-fluorophores...again. In the beginning, we talked about the relationship between a photon's wavelength and energy, how... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog PostPublishedJuly 19, 2016, 2:30 p.m....energy transfer values you can expect, thereby establishing the dynamic range of your FRET assay (Figure...PubMed Central PMCID: PMC2735349. 8. Miyawaki, Atsushi, et al. "Dynamic and quantitative Ca2+ measurements... -
Selecting Your Plasmid Purification Kit
TypeBlog PostPublishedMay 27, 2025, 1:15 p.m....plasmid DNA from your cleared lysate to a substrate, washing away left-over debris that carried over from the...bottles and the overall weight of the package during shipping. Of the kits reviewed here, NEB’s Monarch®, QIAGEN... -
How to Deposit Your Plasmids with Addgene
TypeBlog PostPublishedFeb. 27, 2024, 2:15 p.m....Principal Investigator (PI), provide us with your shipping address for deposit materials, and agree to our...prepare your plasmids and a prepaid label with shipping materials to send your plasmids to us. Essentially... -
Addgene’s First AAV Data Hub Challenge
TypeBlog PostPublishedSept. 20, 2022, 1:15 p.m....award. All participants will receive an Addgene t-shirt. Our judges, Drs. Connie Cepko, Ian Wickersham... -
Welcome to the Lab Bench! New Protocol Series Helps Trainees Get Started in the Lab
TypeBlog PostPublishedSept. 7, 2021, 1:15 p.m....Intro to the Lab Bench series was born from the shift to remote learning due to COVID-19. We had many ... -
CRISPR Guide Update
TypeBlog PostPublishedOct. 10, 2024, 1:15 p.m....widely adopted in the past few years, as well as refreshing the existing content. Access the CRISPR Guide... -
Addgene is Stopping Use of X (formerly Twitter)
TypeBlog PostPublishedJan. 11, 2024, 2:15 p.m.... nonprofit. Recent remarks and actions by X’s leadership conflict with our values. For this reason, our... -
We’re Going Viral This Week!
TypeBlog PostPublishedOct. 14, 2019, 1 p.m.... the sharing of reagents and data, we’re also publishing research. Addgene’s research team also investigates... -
New FLIS Plasmid (April Fools 2022)
TypeBlog PostPublishedApril 1, 2022, 1:15 p.m....tiny creatures do tend to come with a rather astonishing ability to...well, annoy. Fly-related Issues... -
Twelve Amazing Guest Blog Contributors!
TypeBlog PostPublishedNov. 26, 2024, 2:15 p.m....experience working at Addgene. An Addgene Summer Internship Matteo Tardelli, who added to our career advice... -
8 Great Depositor Research Stories
TypeBlog PostPublishedDec. 15, 2022, 2:15 p.m....Cornering the Goldilocks Antibody: Guest blogger Ava Shipman explains how the Wu lab is screening over 3,000... -
Addgene Protocols Go Viral: AAV Purification and Titration
TypeBlog PostPublishedJune 13, 2019, 12:19 p.m....rolling out our viral service in 2016, Addgene has shipped thousands of AAV and lentiviral preps to scientists... -
5 Science Rap Videos & Their Creator: Tom McFadden
TypeBlog PostPublishedFeb. 9, 2017, 3:30 p.m....videos to teach students around the globe and is pushing SciComm further with his new company, Science with... -
Podcast: A Malawian Professor's Path to Biotech Research
TypeBlog PostPublishedMay 24, 2016, 2:30 p.m....University with the backing of a prestigious Fulbright Fellowship. Dr. Kwapata now works as a lecturer in molecular... -
Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More
TypeBlog PostPublishedNov. 15, 2016, 3:30 p.m....Lentivirus Learn about Other Members of the Addgene Leadership Team ... -
Podcast: Interview with Addgene's Founders
TypeBlog PostPublishedApril 13, 2018, 12:51 p.m....Developing Addgene as a modern e-commerce company and establishing credibility Chapter 5 - Realizing that Addgene... -
Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service
TypeBlog PostPublishedJuly 28, 2020, 1:15 p.m....about the cloning grade DNA beta project We will ship you 2 µg of DNA in 20 µl of Tris buffer suitable...