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We narrowed to 466 results for: SHI

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Showing: 261 - 280 of 466 results

  1. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...experimental condition you could look for a slight size shift in the protein band before and after treatment.  ...0003-2697(81)90281-5 Pillai-Kastoori L, Heaton S, Shiflett SD, Roberts AC, Solache A, Schutz-Geschwender ...

  2. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ... 8760896. PubMed Central PMCID: PMC146030. 26. Nishida, Hirokazu, et al. "Overexpression, purification...Addgene Blog Learn New Plasmid Cloning Techniques Pushing the Limits of DNA Assembly Plasmid Cloning by PCR...

  3. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...member who knows how the physical kits are made and shipped; Chiara, the tech transfer expert who understands...critical features based on my own experience: Leadership Few things are more frustrating than being on...

  5. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...inside the cells and are thus typically used for distinguishing live from dead cells. The combination of antibody-fluorophores...again. In the beginning, we talked about the relationship between a photon's wavelength and energy, how...

  6. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...channels. In a typical assay a user will compare the shifting intensity of events. Since intensity positively...positively correlates with expression, the further the shift of a cell from the negative control, the higher ...

  7. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...Dronpa protein (named after dron, a ninja term for vanishing, and pa, for photoactivation) (6). Dronpa is photoactivated...PMID:16550175. 6.Ando, Ryoko, Hideaki Mizuno, and Atsushi Miyawaki. "Regulated fast nucleocytoplasmic shuttling...

  8. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...energy transfer values you can expect, thereby establishing the dynamic range of your FRET assay (Figure...PubMed Central PMCID: PMC2735349. 8. Miyawaki, Atsushi, et al. "Dynamic and quantitative Ca2+ measurements...

  9. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...plasmid DNA from your cleared lysate to a substrate, washing away left-over debris that carried over from the...bottles and the overall weight of the package during shipping. Of the kits reviewed here, NEB’s Monarch®, QIAGEN...

  10. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...Principal Investigator (PI), provide us with your shipping address for deposit materials, and agree to our...prepare your plasmids and a prepaid label with shipping materials to send your plasmids to us. Essentially...

  11. Addgene’s First AAV Data Hub Challenge

    Type
    Blog Post
    Published
    Sept. 20, 2022, 1:15 p.m.
    ...award. All participants will receive an Addgene t-shirt.  Our judges, Drs. Connie Cepko, Ian Wickersham...

  13. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...widely adopted in the past few years, as well as refreshing the existing content. Access the CRISPR Guide...

  15. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...tiny creatures do tend to come with a rather astonishing ability to...well, annoy. Fly-related Issues...

  16. We’re Going Viral This Week!

    Type
    Blog Post
    Published
    Oct. 14, 2019, 1 p.m.
    ... the sharing of reagents and data, we’re also publishing research. Addgene’s research team also investigates...

  17. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    Published
    Nov. 26, 2024, 2:15 p.m.
    ...experience working at Addgene. An Addgene Summer Internship Matteo Tardelli, who added to our career advice...

  18. 8 Great Depositor Research Stories

    Type
    Blog Post
    Published
    Dec. 15, 2022, 2:15 p.m.
    ...Cornering the Goldilocks Antibody: Guest blogger Ava Shipman explains how the Wu lab is screening over 3,000...
Showing: 261 - 280 of 466 results