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Showing: 261 - 280 of 571 results

  1. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...plasmid deposit process can be broken down into four simple steps: Send plasmid information to Addgene Material...the Deposit Spreadsheet is the quickest option. Simply download the spreadsheet and copy and paste your...

  2. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ... For some transgenes, transfection optimization simply is not enough to get a sufficient titer; this is...volumes of lentivirus routinely, you may opt for simply increasing the number of 10 cm2 dishes. While this...

  3. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...Lentiviral Expression) system facilitates the simultaneous introduction of multiple genetic alterations... and animal imaging studies. The ability to simultaneously alter multiple genes via infection with a single...

  6. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...CRISPR is a simple and versatile tool for genome engineering, but its utility is dependent on its ability... albino mice ranging from 11% to 100%, a level similar to other approaches.  Nanoblades can also be used...

  7. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ...their parents. Here’s how to cross mice, using a simple example with the goal is to generate mice that ...raised in the same environment and will harbor similar epigenetic modifications. Special breeding cases...

  11. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...task, action, and results. While this method seems simple, I found it hard to remember to describe all four...answers rings true for you, go ahead and give a similar answer in a following interview. Expect to be tired...

  12. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...therapy currently in clinical trials is somewhat similar (Hussein et al). It employs Cas9 to cut at two ...therapy, since TTR is primarily made in the liver. Similarly, for cardiovascular disease approaches targeting...

  13. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ..., Scientific American or National Geographic. Similarly, media fellowships from the British Science Association... Media Production at Imperial College London. Similarly, the University of Cardiff offers an MSc in Science...

  14. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...OpenWorm project. The main goal of OpenWorm is to simulate C. elegans as a computer model. When I first heard...genome. While C. elegans are often thought of as simple organisms , they have many levels of complexity...

  15. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...administered through the tail vein — many researchers will simply refer to these as “tail vein injections.” However...Intranasal instillation Intranasal instillation is very similar to its intratracheal counterpart, as both procedures...

  16. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...see if any other fluorescent proteins display a similar effect (many do). Fusing EGFP to the self-labeling... tools, they’re optimistic that MagLOV can be similarly adaptable into future magnetogenetic tools.  Embracing...

  17. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ... helper viruses like adenovirus (Ad) or herpes simplex virus to facilitate efficient replication. The ...purify your rAAV from your supernatant, you can simply use polyethylene glycol (PEG). We add 25 mL of ...

  18. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ... tends to go something like this:   However, as simply as it seems laid out above, there are many places...potential programs and universities stressful or simply not feasible. Through the virtual systems created...

  19. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...figure below). The double-MMEJ strategy looks very similar to HDR, but it is accomplished using much smaller... of MMEJ. This double MMEJ strategy looks very similar to HDR, but it is completed using much shorter ...
Showing: 261 - 280 of 571 results