We narrowed to 353 results for: Sele
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TypeBlog PostPublishedJuly 22, 2014, 5:46 p.m....innovation in plant synthetic biology. In our case, we selected a project in a field with 30 years of basic research...
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Antibodies 101: Conjugation
TypeBlog PostPublishedMarch 26, 2024, 1:15 p.m....antibody yourself allows you to consider a broader selection of antibodies for your experiments. Many of ... -
RNA Extraction Without A Kit
TypeBlog PostPublishedMarch 24, 2020, 1:15 p.m....the RNA using lithium chloride. LiCl solutions selectively precipitate RNA, but not DNA and proteins. Lastly... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog PostPublishedMarch 3, 2020, 2:15 p.m....including terminators and promoters, CRISPRi parts, selectable markers, transformation acceptor vectors, and... -
Antibodies 101: Secondary Antibodies
TypeBlog PostPublishedDec. 21, 2021, 2:15 p.m....secondary antibody needs careful consideration before selection. If all the primary antibodies used are mouse ... -
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy
TypeBlog PostPublishedApril 13, 2017, 2:30 p.m....inactive form unless autophagy is induced. Then non-selective macroautophagy of the cytosol leads to accumulation... -
Human Germline Editing Using CRISPR
TypeBlog PostPublishedAug. 10, 2017, 2:19 p.m....cells.They also conducted whole genome sequencing in selected control and CRISPR-Cas9 injected embryos. In all... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog PostPublishedMarch 19, 2019, 1:31 p.m....mutated it. Using a robotic picking approach to select cells with variant characteristics they wanted,... -
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics
TypeBlog PostPublishedNov. 3, 2020, 2:15 p.m....sequences. Thirty of these remaining sequences were selected for characterization with patch clamp electrophysiology... -
Summer SciComm: Preprints
TypeBlog PostPublishedAug. 16, 2022, 1:15 p.m.... P, Peters I (2021) Motivations, concerns and selection biases when posting preprints: a survey of bioRxiv... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog PostPublishedNov. 24, 2015, 3:30 p.m....co-opted to control gene expression, he could then selectively manipulate only GFP-labeled cells. Once Tang ... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog PostPublishedSept. 22, 2015, 2:30 p.m....resistant to drug treatment and that these are selected for during the treatment process. The authors ... -
5 Steps to Writing Grant Proposals for Grad Students
TypeBlog PostPublishedFeb. 11, 2014, 12:13 p.m....eligibility information, submission information, selection criteria, and award administration information... -
A Look at Addgene's QC Process
TypeBlog PostPublishedMay 6, 2025, 1:15 p.m....information provided and vector type Any tags, selectable markers, and fusion proteins listed for each ... -
Design Tips for Prime Editing
TypeBlog PostPublishedJan. 23, 2025, 2:15 p.m.... by the developer of PRIDICT, which helps users select pegRNA designs that are most likely to drive efficient... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog PostPublishedOct. 8, 2024, 1:15 p.m....them off. While antibiotic resistance-mediated selection is indispensable for cloning work, antibiotics... -
Antibodies 101: Choosing the Right Isotype
TypeBlog PostPublishedApril 6, 2023, 3 p.m.... 2). Table 2: Secondary antibody selections for a four-antibody panel, staining in parallel... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog PostPublishedDec. 14, 2021, 2:15 p.m....removable dCas9 expression and pLM-CMV-R-Cre. Selection for cells stably and highly expressing these constructs... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog PostPublishedJuly 12, 2021, 1:15 p.m....contain an IRES-Puro marker for stable cell clone selection. For more information on these plasmids and a ... -
Overcoming the Challenges of Lentiviral Production
TypeBlog PostPublishedAug. 7, 2018, 12:31 p.m....produce virus of a usable titer, or fine-tuning selection and expression in your target cell line, researchers...