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We narrowed to 816 results for: ache

Showing: 261 - 280 of 816 results

  1. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...light used to activate the first PA-FP does not photobleach the second, and vice versa. Finally, as every...contrast, PA-FPs offer a reduced potential for photobleaching and phototoxicity that standard FPs might induce... PA-FPs is photoactivated, imaged, and then photobleached. Additional PA-FP molecules are then activated...still do not emit the large number of photons achievable with photoswitchable dyes. Correlative light ...297.5588 (2002): 1873-1877. PubMed PMID:12228718. 2.Subach, Fedor V., et al. "Photoactivatable mCherry for...

  2. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ...consensus lentiviral expression vector and attached a DNA barcode to each to make the library usable for pooled...proliferation, survival, differentiation, and apoptosis. For each of these 17 pathways, they identified a set of one...researchers didn’t have to discover new ways to activate each of these pathways. Rather, they relied on published...

  3. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    Published
    June 8, 2023, 1:15 p.m.
    ...method that uses iterative staining and chemical bleaching to allow for high-resolution imaging without degrading...IBEX: An iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues...). IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells... Rachel Leeson...

  4. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...2014). Table 1. Summary of Cas9 domains, the lobe each domain belongs to, and their roles. Lobe Domain...crRNA:tracrRNA duplex — Cas9’s two lobes move toward each other into the “closed” conformation shown in Figure...test DNA bases that are already snugly paired to each other? At this point, the protein can switch between...guide RNA (Cofsky et al., 2022; Osuka et al., 2018). Each matching DNA:RNA pair gives just enough stability...own advantages—and blind spots—to these questions. Each new structural insight also spurs the next generation...

  5. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ..., I reached out to Amy with exciting news. I managed to secure a virtual summer program, teaching me the.... A capstone project is a major project given to each intern to complete and present on.    Figure...report shared my work by breaking down the process of each day, my background, observations, etc. On my final...

  6. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...users have recently begun to develop CRISPR-based approaches for studying gene function in these systems. ...use in a fetal-lung derived organoid system, the approach is intended to be amenable to organoids derived...tissue-types. By bringing together CRISPR-based approaches and organoid models, toolkits like this one provide...trigger seed germination of parasitic plants, this approach was also used to build and validate a ratiometric...

  7. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

    Type
    Blog Post
    Published
    July 2, 2015, 8:37 p.m.
    ...University and entered a new role here at Addgene as an Outreach Scientist. I used to spend my days (and often ...This is particularly true in my position as an Outreach Scientist where I spend a large amount of my time...communicator that got me here! Thus far being an Outreach Scientist at Addgene has been awesome. The process...researchers gain doing PhDs (i.e. technical writing, teaching, presenting, analytical thinking, time management...

  8. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...another method Transfection and transduction seem to achieve similar outcomes, so why and when should you opt...Second, what is the biological outcome you want to achieve? For example, do you want the nucleic acid cargo...work is that you can swap out your virus type to achieve different biological outcomes. For example, some...targeting of that virus, which can’t be easily achieved with alternative methods. Ready, set, transduce...

  9. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...disease front. Viruses survive by hijacking the machinery of the cells they infect. Once they enter a cell...delivered to the cell. The cell then uses its own machinery to replicate the viral genome and proteins. This... growing number of Viral Vectors 101 posts about each type, so be sure to check our blog, or our Viral... Rachel Leeson...

  10. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    Published
    Nov. 12, 2019, 2 p.m.
    ... unexplored. Using new single-cell sequencing approaches, the lab was able to eliminate the need for axenic...YNP, it took some time to map a route that would reach all of the remote areas, including one hot spring...poles to collect water samples from a distance. At each collection site, we took GPS coordinates, water ...was separated into smaller samples of 5-10 cells each for sequencing, allowing for lower complexity with...

  11. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ...assays, in vitro transduction, and silver stain approaches we show that there is no difference in purity...so excited? For starters, we believe that this approach can be adapted by vector cores and laboratories...investigated 3 serotypes it’s possible that this approach will be beneficial for a host of other AAV serotypes...serotypes; it will be exciting to test this approach with different capsids. Most importantly, because ...

  13. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    Published
    June 27, 2018, 1:16 p.m.
    ...surface. Their toolkit includes a set of plasmids each containing an anti-GFP nanobody fused with a different...genomic locus: Caspex  Article contributed by Susanna Bachle  Listen to the Caspex podcast segment Steven Carr...Scientist in the group, developed a CRISPR-based approach to detect proteins that associate with specific...Sontheimer and his group from the University of Massachusetts Medical School have developed a group of plasmids...

  14. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    Published
    Sept. 5, 2019, 1:06 p.m.
    ...Below, you will find some easy talking points for broaching this subject with your institution’s tech transfer...Agreements. By default, your TTO must sign an agreement each time a material is being requested or deposited....Reception and Master Deposit Agreements, your TTO signs each agreement just once, covering nearly all future ...University of California, Irvine University of Massachusetts, Worcester  University of Nebraska Medical Center...

  15. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...chromosomal DNA, but they still rely on the host machinery to make additional copies. ori sequences are generally...lower temperatures and allows the replication machinery room to come in and get busy making copies.   ...incompatible because they will compete for the same machinery, creating an unstable and unpredictable environment...higher copy numbers since the bacteria have just reached stationary phase, but the cells have not started...

  16. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...Presentation Experience”, “Career Achievements”, and “Academic Achievements”. Leaning my CV more towards me... business friendly CV that would focus on my achievements, capabilities, courses and office skills. I ... great introduction to the financial world. It teaches you about selling products which is an important...

  17. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...you don’t have a formal connection, you can just reach out to start the conversation. Many researchers ...establishing collaborations, so it doesn’t hurt to reach out and ask if they would be willing to host you...available to directly help you during your visit. Reach out to your bench mentor (if you have one) to communicate...of their way to help you with your research and teach you new techniques. Show up on time (don’t make ...

  18. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ...By Susanna Bachle and Ina Ersing Seeding Labs and Addgene collaborate to provide scientific resources... related to PZA-resistance by using different approaches, including CRISPR and recombination-mediated ...fluorescence microscopy and FACS. In an additional approach, plasmids such as pACBSR-hyg and pFLP-hyg will...community organizations and workforce projects to reach audiences beyond our plasmid depositors and requesters...

  19. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...guides – including an excellent series of posts by Joachim Goedhart on the Addgene blog – outlining various...with a valid email address).  One may also simply attach important references that serve to further characterize...better way to store and represent within-study photobleaching comparisons.  We very much appreciate feedback...Techniques - Which is best for me? A Practical Approach to Choosing the B(right)est Fluorescent Protein...

  20. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...while the alcohol used in the staining process bleached another, Klebsiella pneumoniae. After realizing...safranin, as a counterstain to help visualize the bleached bacteria. The chemistry behind Gram’s crystal ...Society for Microbiology (2005). https://asm.org/getattachment/5c95a063-326b-4b2f-98ce-001de9a5ece3/gram-stain-protocol...
Showing: 261 - 280 of 816 results