We narrowed to 284 results for: cki
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TypeBlog PostPublishedMay 5, 2020, 1:15 p.m.... so, the WHO recommends one strategy: testing, tracking, and social distancing. With countries like South...
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The Fluorescent Vegetables in Aptamer Soup
TypeBlog PostPublishedJan. 29, 2019, 2:27 p.m....bright, high-affinity complex for RNA labeling and tracking." ACS chemical biology 9.10 (2014): 2412-2420.... -
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9
TypeBlog PostPublishedOct. 11, 2016, 2:30 p.m....different from how it’s used in other systems. Luckily, you don’t have to look far for plasmids that have... -
How to Design Your gRNA for CRISPR Genome Editing
TypeBlog PostPublishedSept. 24, 2020, 1:15 p.m....gRNAs to choose from. With so many possibilities, picking a gRNA with an optimized sequence is of primary... -
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility
TypeBlog PostPublishedMay 18, 2018, 7:32 p.m....methods section found within a published manuscript. Luckily, many organizations have been working to provide... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog PostPublishedJune 1, 2023, 1:15 p.m.... factors can also affect your AAV experiments. Luckily, the other posts in our Viral Vectors 101 series... -
CRISPR 101: Off-Target Effects
TypeBlog PostPublishedFeb. 8, 2024, 2:15 p.m....enzyme swaps will only reduce off targets where nicking/cleaving of DNA occurs, not for deadCas activator... -
A Control for All Seasons
TypeBlog PostPublishedOct. 5, 2023, 1:15 p.m....noise. This can be done either by modifying the blocking conditions or by substituting for a more specific... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog PostPublishedJuly 12, 2016, 2:30 p.m...., Nakauchi H (2018) Intra-embryo Gene Cassette Knockin by CRISPR/Cas9-Mediated Genome Editing with Adeno-Associated... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog PostPublishedNov. 6, 2015, 3:30 p.m....2014-15, University of Oviedo): “I couldn’t be luckier with my mentor, since she does research at UK in... -
Finding Your Science Policy Path
TypeBlog PostPublishedSept. 22, 2020, 1:15 p.m....Relationship building is key in science policy. Luckily, there are many mechanisms in place to ensure your... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog PostPublishedOct. 17, 2019, 12:28 p.m....inversion, and then lox sites excision, therefore locking the opsin into the correct orientation to be transcribed... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog PostPublishedApril 16, 2024, 1:15 p.m....time points. Figure 3: Single-molecule tracking of actin filament assembly-regulating proteins... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog PostPublishedMarch 6, 2018, 1:49 p.m.... a method relies on accurate ligation of nicks lacking gaps or mismatched base pairs (as in the case for... -
Transferable Skills Guide: Teamwork
TypeBlog PostPublishedNov. 28, 2017, 2 p.m....yourself. Instead of thinking “What’s my angle on attacking this problem?”, try thinking “Who can I assemble... -
Antibodies 101: Antibody Engineering and Directed Evolution
TypeBlog PostPublishedAug. 5, 2025, 1:15 p.m....8_2 McMahon, C., Baier, A. S., Pascolutti, R., Wegrecki, M., Zheng, S., Ong, J. X., Erlandson, S. C., ... -
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering
TypeBlog PostPublishedDec. 15, 2016, 3:57 p.m....Read about Homology Directed Repair Use Knockout/Knockin Plasmids to Disrupt Genes in Mammalian Cells CRISPR... -
Antibodies 101: Flow Cytometry
TypeBlog PostPublishedJuly 20, 2021, 1:15 p.m....43:404–409 . https://doi.org/10.1063/1.1685647 McKinnon KM (2018) Flow Cytometry: An Overview. Current... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog PostPublishedJuly 11, 2017, 2:30 p.m....like to investigate in your next CRISPR screen. Luckily, the Green Listed software can help you do just... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog PostPublishedSept. 10, 2015, 2:30 p.m....(not expressing the opsin) are also available. Picking your opsin You must consider whether you wish ...