Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 533 results for: erf

Showing: 261 - 280 of 533 results

  1. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...restriction enzymes (Weber et al., 2011). It can be performed at multiple levels to generate anything from individual... tags, connectors, cDNAs, and more to find the perfect combination. The EcoFlex Kit has a wide array of...

  3. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...these types of experiments easier and faster to perform. Introduction to flow cytometry Flow cytometry ...containing a negative cell is discarded. FACS can be performed aseptically allowing users to culture the cells...

  4. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...quantity is fairly self explanatory and achieved by performing different sized preps, such as miniprep, midiprep...bead- and column-based DNA purification methods perform well in terms of quality and yield. Bead-based ...

  5. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...hypothesized that the adenosine deaminase could also perform cytidine (C) deamination, converting the C base...uracil (U). This is the same chemical reaction performed by CBEs. However, most CBEs include an inhibitor...

  7. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...of viral transgenes in iPSC-derived cells can interfere with their differentiation potential. Finally,...to purify the reprogramming proteins needed to perform these experiments. Assays for iPSCs Characterization...

  8. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...combinations to optimize both fusion proteins. The best performing combination was NLS-dCas9-trCIB1 and NLSx3-CRYPHR-p65...AcrIIA4, this unfolding conformational shift can interfere with activity. The resulting system was coined...

  9. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...most machines have a protocol that automatically performs the normalization step.  Machines Most general-use...the results are correct. Primers and probes can interfere with amplification reactions, and reagents can...

  10. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...different than the validation assays, we recommend performing your own validation assays. If you’re using previously...interest is not available in a pure form, you can perform relative quantification, which measures protein...

  12. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ...CRISPR, Addgene gave to me: CRISPR activation and interference screens for primary human T cells🎵 On the 3rd...

  13. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ... day of CRISPR, Addgene gave to me: MUCICAT to perform multicopy chromosomal integration.  On the tenth...

  14. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ...VSV-G envelope protein under the control of the powerful mammalian CMV promoter. Envelope proteins are ...

  16. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    Published
    Nov. 26, 2024, 2:15 p.m.
    ...deposits from Arlene Sharpe’s lab. X-Chime and Performing In Vivo CRISPR Screens Using the FITS Approach...

  19. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ...Falcon tube trees to giant paper mache Krampus performing routine lab maintenance tasks. Hot tip: make ...
Showing: 261 - 280 of 533 results