We narrowed to 513 results for: gats

...then need to be purified by density gradient centrifugation (e.g., iodixanol) using a high-speed ultracentrifuge...ddPCRAAV Purification by Iodixanol Gradient UltracentrifugationAAV Production in HEK293 Cells...

...lead to improper protein localization, protein aggregation, or disruption of normal protein function, and... functionally unique repair templates. A final ligation reaction correctly assembles the final targeting...

...software confirms the serotype of the prep by interrogating the VGS data for small predetermined seed sequences...could lead to transgene expression in recombinase-negative animals, producing misleading results.  To date...

...direct molecular interaction without further investigation. One fluorescent microscopy method commonly ... to fluorescently label a wide variety of gram negative bacteria (West Virginia University School of Medicine...

...made me happy. Grad school can provide plenty of negativity, so I volunteered at a place that made me happy...recharged. The fun change of pace helped break up the negativity and allowed me to focus on the positive. I went...

...the genome of the pathogenic fungus Aspergillus fumigatus, which has been difficult to modify due to NHEJ...microhomology-mediated end joining in Aspergillus fumigatus.” Fungal Genet Biol. 86: 47-57. PMID: 26701308...

...? Depositing lab: The name of the Principal Investigator (PI) in whose lab the construct was made. The...may require strains such as ccdB survival (for Gateway plasmids).  We also like to store plasmids that...

... lead to a wealth of additional experiments investigating the role of individual genes or entire pathways...genome-wide screening. Avoid false positives and false negatives – As with any experiment, the use of appropriate...

... placing orders per day across regions. Our investigation of model fit revealed some potential instabilities...University; and Howard Hughes Medical Institute Investigator. Liu’s research integrates chemistry and evolution...

...professional. Dr. Cook served as a principal investigator for several research grants. One of the benefits...benefits of being a principal investigator is that you are asked to review grant proposals from others. ...

...sentiments were echoed in the other testimonies we gathered. The program definitely caused a positive stir...Iwasa, Shawnna Buttery, Kat Milligan-Myhre, and Pragati Chengappa.  Financial and moral supporters Denise...

...Also, overexpression of FPs can trigger protein aggregation which is toxic for the cells and affects the ...Furthermore, many of these FPs are cytotoxic, prone to aggregation and cross-react with anti-GFP antibodies, making...

...translation. The ribosome may slow and pause during elongation which may actually be necessary for proper protein...Wobble base-pairing slows in vivo translation elongation in metazoans. RNA 17:2063–2073 . https://doi....

...Virologists too have benefited from the ability to propagate viruses in cell culture; if there were no cells...line consisting of records of the origin and propagation of the line, and can include assays for authentication...

...of several popular strains of E. coli for DNA propagation. While great for cloning purposes, these E. coli...hydrophobic regions or multiple disulfide bonds may aggregate and become insoluble. These insoluble globs of...

...used with Clover pGWF1 Cyan & Yellow Bacterial Gateway-compatible vector to express a gene of interest... is difficult to address especially when you investigate unknown molecular complexes. But it is a parameter...

...expression mRNA Strong mammalian expression from human elongation factor 1 alpha Constitutive  Tends to give consistent...TEF1 General expression mRNA Yeast transcription elongation factor promoter Constitutive  Analogous to mammalian...

...faculty search committees. Having successfully navigated the trials and tribulations of the process, I’...It helps to come from the lab of a well-known investigator. If you only have middle author papers or no...

...delivered through transfection of naked DNA. Other investigators who had been working to develop high efficiency.... "Intact cohesion, anaphase, and chromosome segregation in human cells harboring tumor-derived mutations...

...has a positive prediction rate of 95% and a 100% negative prediction rate. It is also the fastest test that...University. Shravanti Suresh is a PhD candidate who investigates cellular aspects of CRISPR/Cas immune systems...