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We narrowed to 515 results for: gats

Showing: 261 - 280 of 515 results
  1. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...the genome of the pathogenic fungus Aspergillus fumigatus, which has been difficult to modify due to NHEJ...microhomology-mediated end joining in Aspergillus fumigatus.” Fungal Genet Biol. 86: 47-57. PMID: 26701308...
  2. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...? Depositing lab: The name of the Principal Investigator (PI) in whose lab the construct was made. The...may require strains such as ccdB survival (for Gateway plasmids).  We also like to store plasmids that...
  3. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...lead to improper protein localization, protein aggregation, or disruption of normal protein function, and... functionally unique repair templates. A final ligation reaction correctly assembles the final targeting...
  4. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...software confirms the serotype of the prep by interrogating the VGS data for small predetermined seed sequences...could lead to transgene expression in recombinase-negative animals, producing misleading results.  To date...
  5. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...elaborate if your audience seems captivated. Expertly navigate questions Answering questions is arguably the ..., so let us know your own ways to successfully navigate poster sessions with ease. Many thanks to our...
  6. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...So you’ve done the research, gathered up your data into an exciting story, and are ready to present your... information to prepare for the next meeting. Navigating talks Before the conference Is the conference...
  7. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ... lead to a wealth of additional experiments investigating the role of individual genes or entire pathways...genome-wide screening. Avoid false positives and false negatives – As with any experiment, the use of appropriate...
  8. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...of the run, each droplet is read as positive or negative for each reaction, math happens (this time, Poisson...accurately, and raw data should be checked to confirm negative samples and, if unexpected, verified by running...
  9. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...professional. Dr. Cook served as a principal investigator for several research grants. One of the benefits...benefits of being a principal investigator is that you are asked to review grant proposals from others. ...
  10. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ... placing orders per day across regions. Our investigation of model fit revealed some potential instabilities...University; and Howard Hughes Medical Institute Investigator. Liu’s research integrates chemistry and evolution...
  11. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...Also, overexpression of FPs can trigger protein aggregation which is toxic for the cells and affects the ...Furthermore, many of these FPs are cytotoxic, prone to aggregation and cross-react with anti-GFP antibodies, making...
  12. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...Virologists too have benefited from the ability to propagate viruses in cell culture; if there were no cells...line consisting of records of the origin and propagation of the line, and can include assays for authentication...
  13. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...of several popular strains of E. coli for DNA propagation. While great for cloning purposes, these E. coli...hydrophobic regions or multiple disulfide bonds may aggregate and become insoluble. These insoluble globs of...
  14. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...used with Clover pGWF1 Cyan & Yellow Bacterial Gateway-compatible vector to express a gene of interest... is difficult to address especially when you investigate unknown molecular complexes. But it is a parameter...
  15. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...translation. The ribosome may slow and pause during elongation which may actually be necessary for proper protein...Wobble base-pairing slows in vivo translation elongation in metazoans. RNA 17:2063–2073 . https://doi....
  16. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...expression mRNA Strong mammalian expression from human elongation factor 1 alpha Constitutive  Tends to give consistent...TEF1 General expression mRNA Yeast transcription elongation factor promoter Constitutive  Analogous to mammalian...
  17. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...delivered through transfection of naked DNA. Other investigators who had been working to develop high efficiency.... "Intact cohesion, anaphase, and chromosome segregation in human cells harboring tumor-derived mutations...
  18. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ... IDLVs were first discovered by researchers investigating HIV integrase mutants in the early 1990s (Leavitt...of SCID-X1 patients. The Journal of Clinical Investigation, 118(9), 3143–3150. https://doi.org/10.1172/...
Showing: 261 - 280 of 515 results