Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 415 results for: genome

Showing: 261 - 280 of 415 results

  1. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ..., green chemistry and understanding the minimal genome. Many of these techniques require more complex ...Assembly® is a registered trademark of Synthetic Genomics, Inc. One or more of these products are covered...

  2. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ... cell lines from becoming the new HeLa cells?), genome engineering and stem cells, and the difficulty ...progenitors into a pig. In stark contrast to recent genome engineering technologies, stem cell techniques ...

  3. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...is causal for a given disease. CRISPR has made genomic SDM relatively straightforward, but plasmid-based...separate plasmid or be inserted into the bacterial genome. REPLACR-mutagenesis starts out in familiar territory...

  4. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...technologies to functionally investigate the human genome. Doench says he enjoys working on technology development...functional characterization of specific edits to the genome. Such characterization can be done in a low throughput...

  5. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ...attended the recent Keystone Meeting "Precision Genome Engineering and Synthetic Biology". His reflections...user forums such as http://www.genome-engineering.org and http://www.egenome.org." At the Keystone Meeting...

  6. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...ion channels, despite having an otherwise normal genomic background, can even lead to the formation of whole...reprogrammed regenerating tissues without using genome engineering. Planarian worms cut into three segments... 2018) available at Addgene: MerMAID2 - A metagenomically discovered marine anion-conducting channelrhodopsin...

  7. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...or if you want to amplify sequences from a large genome organisms like mouse or human. There are several...amplifying a template, particularly from a large genome, you can recheck your primer design or optimize...

  8. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...resource from Jennifer Doudna’s lab at the Innovative Genomics Institute. What is CasPEDIA? CasPEDIA is an online...Beyond SpCas9 More resources from the Innovative Genomics Institute      CRISPRpedia IGI’s digital resources...

  9. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...researchers have adapted the components for use in genome manipulation. Cre recombinase, originally named... sequence does not occur naturally in any known genome other than P1 phage, and is long enough that there...

  10. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...William RA, et al. "Serine recombinases as tools for genome engineering." Methods 53.4 (2011): 372-379. PubMed...Blog Learn How Piggy Bac Vectors Can Be Used for Genome Editing Learn about Michael Koeris' Journey from... Pages Check Out More Lu Lab Plasmids Find More Genome Engineering Tools  ...

  11. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...embryo derived-ES cells.They also conducted whole genome sequencing in selected control and CRISPR-Cas9 ...predicted off-target effects, they limited their whole genome sequencing to a small percentage of edited embryos...

  13. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...transcription. However, with scAAV the effective genome size usable is shortened (from ~4.8 kB [8] to ~...performance profiles and should be suitable for genome editing. These vectors can be used to improve targeted...adeno-associated virus serotypes: impact of larger genomes on infectivity and postentry steps." Journal of...

  14. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...generation packaging systems further split the viral genome by removing the viral Rev gene from the packaging...This system improves safety by dividing the viral genome into additional plasmids thereby increasing the...

  15. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...this protein must undergo as it interrogates your genome. If you are interested in experiencing Cas9 up ...Addgene Blog Learn All about CRISPR Use CRISPR for Genome Visualization Tips for a First Time CRISPR User...

  16. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...spreading through wild populations. Whereas most genomic changes impose a fitness cost and are eliminated...advance enabling RNA-guided gene drives is Cas9 genome editing. Finally, gene drives are an inherently...

  19. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ... 3, and of course the rapidly changing world of genome engineering via CRISPR.  Though the individual ... a thing if CRISPR hadn’t made manipulating the genome cheap and relatively easy? Have all of these things...
Showing: 261 - 280 of 415 results