We narrowed to 406 results for: mag

1. Plasmids 101: TOPO Cloning

   Type

   Blog Post

   Published

   Oct. 27, 2016, 2:30 p.m.

   ... introduce your 3’-end “A” overhang insert, the magic of TOPO cloning happens. Basic procedure Let’s break...

2. Plasmid Cloning by PCR

   Type

   Blog Post

   Published

   March 29, 2016, 2:30 p.m.

   ...that will bind to and replicate it. The following image shows the ends of the ORF and how these are used...

3. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

   Type

   Blog Post

   Published

   Oct. 29, 2015, 2:30 p.m.

   ... Bioblasts? Plasmagenes? In the 1940s and 50s, scientists were working to understand genetic cytoplasmic...

4. Another Pathway into Cells: iTOP

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   Blog Post

   Published

   June 23, 2015, 8:37 p.m.

   ...transduced with iTOP resulting in GFP expression. Image courtesy of Diego D'Astolfo. CRISPR/Cas9: a ...

5. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

   Type

   Blog Post

   Published

   July 21, 2020, 1:15 p.m.

   ...fragments migrate more quickly than larger fragments. Image from OpenWetWare. This method is often used preceding...

6. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

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   Blog Post

   Published

   June 30, 2020, 1:15 p.m.

   ...this interactive activity during their session. Image from Reproducibility for Everyone.  While this...

7. Scientists Map the SARS-CoV-2-Human Interaction Network

   Type

   Blog Post

   Published

   April 9, 2020, 1:15 p.m.

   ...guy has been the best: Thanks, Todd! As you can imagine, it’s also been quite the administrative headache...

8. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

   Type

   Blog Post

   Published

   Nov. 27, 2018, 2 p.m.

   ...laser scanning microscope will allow Tsakanova to image cortical neurons and study changes in neural activity...

9. New and Upcoming Viral Vectors - June 2019

   Type

   Blog Post

   Published

   June 18, 2019, 1:15 p.m.

   ...tracking large populations of neurons in wide-field imaging (Dana et al., 2018). See our full biosensor AAV...

10. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type

    Blog Post

    Published

    June 4, 2019, 1:02 p.m.

    ...the sensor for serotonin by over four orders of magnitude. Unger validated the serotonin sensor with proof-of-principle...

11. The Importance of High Titer for AAV Transductions

    Type

    Blog Post

    Published

    March 1, 2018, 2:11 p.m.

    ...this function is not yet fully understood, we can imagine that this transport is probably not 100% efficient...

12. K. phaffii: Rising to the Occasion

    Type

    Blog Post

    Published

    April 4, 2023, 1 p.m.

    ...DOI: 10.3892/br.2017.941 Bernauer L., et al., Komagataella phaffi as Emerging Model Organisms in Fundamental...

13. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type

    Blog Post

    Published

    April 28, 2021, 1:15 p.m.

    ... The Pomodoro timer is literally a tomato! Image from Gerlos.   Tools for checking grammar and...

14. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type

    Blog Post

    Published

    Feb. 9, 2021, 2:15 p.m.

    ...can still creatively collaborate and brainstorm! Image from Joseph Mucira.   Implement new tools for...

15. "Build Your Plasmid": The Game - Play to Teach

    Type

    Blog Post

    Published

    July 17, 2018, 12:23 p.m.

    ...flashback to time spent playing a card game called “Magic”. There were complicated rules that governed how...

16. R Bodies: Membrane-Rupturing Microscopic Tools

    Type

    Blog Post

    Published

    April 14, 2016, 2:30 p.m.

    ...Attaching the fluorescent protein fusions allowed us to image R bodies inside cells, and these constructs may ...

17. Components of CRISPR/Cas9

    Type

    Blog Post

    Published

    Feb. 2, 2016, 5 p.m.

    ... DNA. Thus, the end result of Cas9-mediated DNA damage is a DSB within the target DNA ~3-4 nucleotides...

18. Synthetic Photobiology: Optogenetics for E. coli

    Type

    Blog Post

    Published

    Sept. 8, 2015, 2:30 p.m.

    ...develop a bacterial camera that prints a chemical image, as well as a genetic method for the computational...

19. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type

    Blog Post

    Published

    Aug. 28, 2014, 3:34 p.m.

    ...BamHI) and run on an agarose gel. The resulting gel image includes a 1kb ladder (lane 1) that has bands ranging...

20. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type

    Blog Post

    Published

    July 28, 2017, 1:30 p.m.

    ...can produce some very beautiful immunofluorescent images like in this paper (2). Metabolic assays such as...