...Realizing that Addgene would be successful and the importance of lentiviral vectors in ensuring that success... Addgene's Future, the research team, and the importance of sharing information/knowledge Outro Many ...

...Seeding Labs, Addgene also feels it’s incredibly important for scientific breakthroughs, reagents, and know-how...continues to face global challenges, we hope to empower those that need to face these challenges locally...

...neutralization or targeted destruction.  Antibodies are composed of two light chains and two heavy chains. The ...repositories, such as Addgene and the DSHB, play an important role in scientific exchange. In addition to ensuring...

...identify what your plasmid contains and other important information. Tip: A lowercase "p" is often used...simple piece of information can often convey many important details. Once you know the backbone a plasmid ...

...: Topic 0:00 - 0:50 : Intro 0:51 - 3:08 : The importance of developing a strong graduate student community...you changed throughout graduate school? - The importance of criticism and feedback 15:38 - 17:06 : Post...

...proteins to migrate…so don’t try eliminating one component entirely. If you are trying to transfer proteins...increases (Figure 3A).  This step is especially important for highly expressed proteins, including “housekeeping...don’t want to skimp on your washing steps! It’s important to let them wash for the full amount of time, ...

...ready-to-use recombinant antibodies collection, currently composed of over 100 antibodies. Everything in this collection...validation data, sharing what works and, equally importantly, what doesn’t. Reports receive a DOI for citation...

...mutations, both in mice and in human cells. But that important advance was quickly followed by even splashier...All this, and scientists are still working out important details in their understanding of how CRISPR/Cas9s...

...mammalian cell lines. Cas9, of course, is the key component of most CRISPR systems and generates double strange...are some additional features. The first and most imporant is that, upstream of the Cbh promoter, this plasmid...

...least one site. Phosphorylation is particularly important for regulating signal transduction and measuring...the nucleus and the cytoplasm, nuclear export and import rates can influence KTR translocation. Work from...

...containing these base editors Spatially and temporally regulated AAV-based genome editing In 2016, Jonathan...AAV-based genome editing system is regulated not only temporally with doxycycline, but it can also be regulated...

...water of HR work. What do you think is the most important resource that Addgene provides the scientific ... how to operate during the pandemic, but it’s important to develop a plan for moving forward. Sooner or...

... genetic circuits, allowing for tighter spatio-temporal control of gene expression within a cell. Thanks..., designer PUF proteins can be fused to other components to lend them many other functions for use in ...

...accelerating scientific discovery. Over 150,000 plasmids empowering researchers worldwide. Countless breakthroughs...characterization data for over 150,000 plasmids, empowering bioinformaticians, data scientists, and developers...

..., regardless of who is paying for them. More importantly, there’s the opportunity cost of your time; an...gRNAs to knockout genes – are essential screening components, the model system and the assay are just as critical...

...#ActualLivingScientists). We also discuss the importance of actionability on the Addgene blog and how ...The SITN Editorial Process -Vini discusses the importance of the editorial process and peer-to-peer feedback...

...patient data to identify mutations of unknown importance in cancer Previously available methods for investigating...passenger mutations from the “active” drivers is important for the development of targeted therapeutic interventions...

...proteins (FPs) are also the foundation of multiple important technologies, including FRET and optogenetics.... itself is photoconvertible, new studies of spatiotemporal dynamics can be undertaken using available ...

...defend the thesis, etc. Photography is critically important in many fields of biology and I believe it could...wider audience for more than just its biological importance. More of Daniel's stunning work can be found ...

... lab’s achievements? Tom Ellis highlights the importance of acknowledging his teams successes. “When PhD...required to achieve success in the lab, it is important to take the occasional breather and acknowledge...