Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 507 results for: otos

Showing: 261 - 280 of 507 results

  1. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...has  developed a proprietary, low cost cloning protocol that he has used for cloning of more than 10,000...plasmid elements Resources on Addgene.org Find protocols for plasmid cloning Read our molecular biology...

  2. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...FPs optimized for oxidizing environments to photoconvertible variants, it seems like GFP is always learning...delivered throughout the nervous system, including the motor cortex, cerebellum, and spinal cord. Cre-DOG is ...

  3. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ... Their goal was to figure out how to optimize protocols for in vivo screening with lentiviral pooled shRNA... a lot of time packing, shipping, and sharing protocols one by one,” and she is excited to deposit the...

  4. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...posts for updates on the most recent deposits, protocols, and tips for using retroviral vectors in your...of your choice. More information and a detailed protocol on how to use the pLKO.1 vector can be found here...

  9. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...a group discussion on the best strategies and protocols for different experiments.  In this blog post...and resources page Browse our molecular biology protocols Read our molecular biology reference ...

  10. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...encoded calcium indicators. Cold Spring Harbor Protocols. 2012 Jun 1;2012(6):pdb-top069609. Palmer AE, ...genetically targetable fluorescent indicators. Nature protocols. 2006 Aug;1(3):1057-65. Resources on Addgene.org...

  11. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...ligand co-developed by Bryan Roth's and Takafumi Minamimoto's lab called deschloroclozapine (DCZ) shows promise...with the IPD of a cation channel, such as the serotonin 3 receptor, will promote depolarization in neurons...

  12. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...anti-mouse antibody. Others recognize specific isotopes within that species, like an anti-mouse IgG antibody...sample. There are species-on-species staining protocols that use antibody fragments to block the endogenous...

  13. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...structures, etc., as well as the presence of a Protospacer Adjacent Motif (PAM) sequence. If the gRNA sequence...genome editing in stem cells using CORRECT. Nat Protoc 12, 329–354 (2017). DOI:10.1038/nprot.2016.171 ...

  15. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...reported the development of a rapid design and prototyping pipeline that can take pathogen sequence information...critical needs and to further develop our rapid prototyping and sensor assembly pipeline for future emerging...

  16. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...that is complementary to the target site, or protospacer, in the genome. Native Cas9 has two catalytic...which cleaves one strand of DNA upon binding the protospacer. The resulting double strand break (DSB) stimulates...

  17. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...Titration of baculovirus can vary depending on the protocol used. Plaque assays are standard but can take ...More resources on Addgene.org Addgene's virus protocols Viral Plasmids and Resources References Shaw, ...

  18. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ..., & Zoltowski, B. D. (2021). Cryptochromes: Photochemical and structural insight into magnetoreception...Matsuoka, R., Kimura, S., Miura, T., Ikoma, T., & Kusamoto, T. (2023) Single-Molecule Magnetoluminescence...

  20. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...error. Used in combination with standard barrier protocols, they can reduce the risk of accidental escape...research have conclusively shown that barrier protocols are vulnerable to human error. And with gene drives...
Showing: 261 - 280 of 507 results