We narrowed to 509 results for: otos

...become slightly denatured during your experimental protocol, using a polyclonal antibody increases the likelihood...Antibody Purification and Storage. Cold Spring Harb Protoc 2019:pdb.top099101. https://doi.org/10.1101/pdb.top099101...

...a proxy for autophagy. Cells are cultured with isotope-labeled amino acids for several hours to several...Protein Oligomerization Seeing Red: Simple GFP Photoconversion Light Sheet Fluorescence Microscopy Additional...

...FPs optimized for oxidizing environments to photoconvertible variants, it seems like GFP is always learning...delivered throughout the nervous system, including the motor cortex, cerebellum, and spinal cord. Cre-DOG is ...

... Their goal was to figure out how to optimize protocols for in vivo screening with lentiviral pooled shRNA... a lot of time packing, shipping, and sharing protocols one by one,” and she is excited to deposit the...

...contact information, and potentially a photograph of yourself. A photo can be very useful in the event that...

...a group discussion on the best strategies and protocols for different experiments.  In this blog post...and resources page Browse our molecular biology protocols Read our molecular biology reference ...

...posts for updates on the most recent deposits, protocols, and tips for using retroviral vectors in your...of your choice. More information and a detailed protocol on how to use the pLKO.1 vector can be found here...

.... Recent studies have shown that the conditions Sotos-type 2 and Marshall Smith Syndrome are due to mutations...more accurate diagnosis of NFIX-related syndromes (Sotos-type 2 and Marshall-Smith) and could be expanded...

...fluorescence intensity due to interactions with photoactivatable fluorescent protein Dronpa. The authors tracked...peak of 519 nm. These proteins have improved photophysical properties and can be used in quenching, FRET...

...Check out our AAV Production Protocol Check out our AAV Purification Protocol ...

...structures, etc., as well as the presence of a Protospacer Adjacent Motif (PAM) sequence. If the gRNA sequence...genome editing in stem cells using CORRECT. Nat Protoc 12, 329–354 (2017). DOI:10.1038/nprot.2016.171 ...

...encoded calcium indicators. Cold Spring Harbor Protocols. 2012 Jun 1;2012(6):pdb-top069609. Palmer AE, ...genetically targetable fluorescent indicators. Nature protocols. 2006 Aug;1(3):1057-65. Resources on Addgene.org...

...ligand co-developed by Bryan Roth's and Takafumi Minamimoto's lab called deschloroclozapine (DCZ) shows promise...with the IPD of a cation channel, such as the serotonin 3 receptor, will promote depolarization in neurons...

...anti-mouse antibody. Others recognize specific isotopes within that species, like an anti-mouse IgG antibody...sample. There are species-on-species staining protocols that use antibody fragments to block the endogenous...

...to ABE7.10. Depending on the position in the protospacer, the editing capacity of the ABE8s ranged from...site in the genomic DNA to contain a specific protospacer adjacent motif (PAM) made up of 5′-NGG-3′ (“N...

...reported the development of a rapid design and prototyping pipeline that can take pathogen sequence information...critical needs and to further develop our rapid prototyping and sensor assembly pipeline for future emerging...

...Titration of baculovirus can vary depending on the protocol used. Plaque assays are standard but can take ...More resources on Addgene.org Addgene's virus protocols Viral Plasmids and Resources References Shaw, ...

..., & Zoltowski, B. D. (2021). Cryptochromes: Photochemical and structural insight into magnetoreception...Matsuoka, R., Kimura, S., Miura, T., Ikoma, T., & Kusamoto, T. (2023) Single-Molecule Magnetoluminescence...

...taking a snapshot of chromatin looping with the photo “developed” by analyzing DNA sequencing data. While... The high resolution and multiplexed chromatin ‘photos’ generated with CAPTURE 2.0 allow us to uncover...

...that is complementary to the target site, or protospacer, in the genome. Native Cas9 has two catalytic...which cleaves one strand of DNA upon binding the protospacer. The resulting double strand break (DSB) stimulates...