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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 628 results for: rel

Showing: 261 - 280 of 628 results

  1. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...Zhang lab’s previously published protocol and its relevant modifications on the pooled library webpage. Disease... that any researcher could use to generate the relevant disease model. Accurate modeling of a disease ...isoforms. Due to the size of the collection, we’re releasing individual plasmids in batches. If there’s something...

  2. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ..., check out this related blog post: Early Career Researcher Toolbox: Finding Relevant Papers. These are...audience. The tool highlights jargon terms or words rarely used in general writing in red, words with a medium...

  3. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...chairs in the hallway for people to take a break and relax. Companies at LabCentral get access to a variety... this is the most important part of this newly released prep kit. In their prior NEBNext Ultra II DNA ...understand? How does the information I’ll be learning relate to me as an individual and Addgene as a company...

  4. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...the virus interacts with in cells. Because viruses rely on the help of human proteins in their lifecycle...other cell types, including more physiologically-relevant systems, to catch all the important interactions...and Facebook. Find more COVID-19 and coronavirus related plasmids and resources at Addgene* Many thanks...

  6. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ..., and they can rupture E. coli spheroplasts to release encapsulated proteins. Video by ACS. For a more...Tunable Protein Piston That Breaks Membranes to Release Encapsulated Cargo. ACS Synth. Biol. (2016). doi...contain microtubule-like structures are bacteria related to Verrucomicrobia. PNAS 97, 1813–1817 (2000). ...

  7. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...performances, and research experiences, you now have merely two pages to convince your potential employer that...when the research topic you have done is closely related to the job you are applying for, in which case ...in Experimental Medicine. She is the Community Relations Director for Curiosity Collider (hyperlink https...

  8. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    Published
    July 6, 2017, 1:59 p.m.
    ...Cancer and the Immune System: Deciphering the Relationship” by our guest blogger Subhadra Jayaraman, a ...platform focused on analysing immune measures and correlating them with tumor mutations and clinical responses...Cancer and the Immune System: Deciphering the Relationship Resources on Addgene.org Browse Cancer Plasmids...

  9. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...protein properties need to be scrutinized under relevant conditions. The properties of fluorescent proteins...several important experimental conditions that are related to the imaging strategy and the sample. Practical...the assay is depicted in figure 1. The strict correlation between the fluorescent protein of interest and...

  10. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...is software product management, and how does it relate to science? In the simplest of terms, a software...project management  Bolster your skills in strong relationship-building and influence. You’ll need a large ...large deal of influence and strong relationship-building in order to rally many stakeholders behind a common...

  11. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...that contain your plasmid backbone, as it often relies on antibiotic resistance. But what about identifying...method of screening is the blue-white screen, which relies on the lacZ gene. lacZ encodes the enzyme 𝛽-galactosidase... functional 𝛽-galactosidase. Blue-white screens rely on vectors where the gene of interest is inserted...

  12. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...Passion? A love of learning? No. Or at least not entirely. What truly unites us all is the frustration of... to the day before, which got rid of the issue entirely. In many cases, the answer isn't to work harder...double-checking details, or just taking a moment to relax. During really stressful days, I would even take...

  13. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...mutant and wild type stem cells into any cell type relevant to the disease in question – cardiomyocytes, adipocytes...olden days," not even five years ago, scientists relied on traditional homologous recombination. That technology...will bind desired sequences. TALENs are much more reliable. If you design them for a particular sequence,...

  14. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...identifies RNA-protein interactions. Both methods rely on the promiscuous E. coli biotin ligase, BirA*,...that promote RNA decay and their upregulation is correlated with poor survival in breast cancer patients.... wild-type sequences and that loss of binding correlated with increased serum iron levels seen in HHCS...

  15. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...targeted Tet-on transgene vector. Degron tags Tagging relevant proteins with a degron tag can provide inducible...endogenously or exogenously expressed protein with the relevant tag and then introducing a specific ligand to ...unstable and quickly degraded. However, if the relevant small molecule is introduced into the system, ...

  16. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...bidirectional transcription. In an uninfected cell, dsRNA rarely circulates, but upon viral infection, this nucleic...barring a loss of mitochondrial integrity that would release them into the cytosol. Fig. 2: Endogenous...upon viral infection. Endogenously produced dsRNA rarely reach this threshold and thus do not typically ...

  17. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...antibodies to antibody fragments to tools completely unrelated to antibodies.  Why use an alternative affinity...either re-develop or re-optimize your assay for an entirely new affinity reagent.    Fig. 1: A comparison... These are antibody mimics and are not closely related to antibodies. Instead, they are derived from a...

  18. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...salaries - in some cases reversing these decisions entirely. At Future of Research, we had been collecting...available (below). However, on November 22nd a preliminary injunction against the FLSA updates was granted...say, salaries are showing surprisingly little correlation with cost of living at the location of the institution...

  19. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ...future prospects. Among the topics discussed, many related to the structure of the biomedical workforce. Quotes...results, not scientists”  These comments are well correlated with published data. For example, in 2010, less...grants through the Sloan foundation for postdoc-related data collection and analysis. He is also a strong...

  20. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...scientists are aware of the benefits of coaching relationships and do not know where to look for a coach. Many...thinking about what your goals are for the coaching relationship. Do you want to change jobs? Grow your professional...the coach to define how you will know that the relationship has been successful – what are the desired outcomes...
Showing: 261 - 280 of 628 results