We narrowed to 855 results for: NTS

...unique tropisms are now available. These include variants with broad CNS or PNS transduction, cell type ... control transgene expression. Gene regulatory elements [promoters and enhancers, miRNA target sites, ... 2021) to evolve new systemic capsids for both rodents and non-human primates (NHPs). These engineered...fluorescent proteins, optogenetic sensors, CRISPR elements, etc... *These are only starting recommendations...not been tested in rats. For more information on reagents and protocols, visit https://clover.caltech.edu...frequently been used for screening candidate regulatory elements such as enhancers and promoters for cell type-...Tabebordbar et al., 2021) method, selecting for variants that were effective in C57BL/6J mice, BALB/cJ ...

...selection easier, as well as highlight some recent advancements within this field. Fundamental principles of...enable highly specific visualization of cellular components, typically a protein of interest. Fluorescent...ligands Effects on protein function and cellular components: It's important to assess how dye-conjugation... function and its propensity to bind cellular components. Some dyes target specific organelles like mitochondria...may prove challenging to stain intracellular compartments in in vivo model systems, so extra care must..., highlighting its reliability for long-term experiments (Salari et al., 2019). Photostability, the ability...experiment types: Different types of fluorescent experiments, such as using fluorescently tagged ligands or...

... Identify reagents you will need, any required training, and a priority list of experiments you hope to...that they may want to consider for future work placements. The chance to learn and collaborate all while...which you can apply to. These structured types of placements often have some form of built-in professional... ensure your expectations are reasonable, your reagents are onsite before you need them, and you are set... plan as well. Making logistical and funding arrangements Visiting scientist positions take commitment...structured visiting program, you will have to make arrangements on your own. This will include, but is not limited...transportation, living expenses, and necessary reagents. Your primary lab may be willing to cover these...

...of your various blot reagents. There are two types of gradients. Protein gradients use dilutions of your...background. Created with biorender.com   Both gradients can be informative, so spend some time thinking...particular question before running one.  Good on gradients? Excellent! Now, let’s head west, intrepid reader...   Ladders If you need very accurate protein measurements, consider using an unstained protein ladder,...conjugated antibody. If you do not need such precise measurements, you can use a colorimetric ladder, visible ... after your transfer. If you are expecting low amounts of protein, make sure your reversible staining ...wet and semi-dry transfers, you can adjust the amounts of alcohol and SDS in your transfer buffers. To...

...chemicals (biotinylation reagents). There are a variety of biotinylation reagents available that differ ...and thus different reagents are useful for biotinylation in different microenvironments. There are also ...organisms for life. Biotin is only synthesized by plants, most bacteria and some fungi, thus mammals must...unaffected by changes in pH, temperature or organic solvents. Uses of biotin in molecular biology Biotin is...protein purification and immunoprecipitation experiments. Biotinylated proteins or complexes can be easily...immunohistochemistry and antibody labelling. In these experiments, biotin is added to a primary or secondary antibody... ideal for signal amplification during these experiments. During detection, a biotinylated reporter enzyme...

...sequencing large amounts of DNA quickly and accurately. We're sure to see many improvements in a variety ...and sequencing gels, as well as their modern equivalents and see what we can learn from their evolution...DNA identity, size, and abundance. If a scientist wants to know if an organism has a particular gene mutation...confirming the results of cloning or knockout experiments to forensics and clinical diagnostics. One example...individual without sickle cell anemia, two DNA fragments are observed because the DNA has the site where...investigate DNA sequence content by comparing the fragments created by different restriction enzyme digests...allows a researcher to properly orient specific fragments of a DNA sequence of interest to create a physical...

... of graduate students who are passionate about communicating science.  ComSciCon's events have 3 main ...apply for our future events. In addition to this annual event, graduate students in different regional...comscicon.com/. All our events are offered free of charge to accepted students, and we pay for travel ...Communicating Science Conference series for graduate students. I believe that communication is the single most...main goals: To help graduate students in all STEM fields become excellent science communicators. To empower...empower graduate students to become ambassadors for their respective fields. To help attendees launch ...practice science communication to more graduate students.   Each June, graduate student leaders in science...

... methods. Across these methods, the following components are needed: γ-Retroviral transfer plasmid encoding...similar to lentiviral packaging methods. The three components described above (envelope, packaging, and transfer...because once in the host cell cytoplasm, the viral components are small enough to enter the nucleus through...slightly different isoforms of these packaging components. Therefore, lentiviruses may not be efficiently...plasmid backbone is meant to be descriptive of the components found within the backbone. For example: pLXSN...particle containing Matrix, Capsid, and Nucleocapsid components. Pol Precursor protein containing Reverse...Reverse Transcriptase and Integrase components. Transfer cPPT Central polypurine tract; recognition...

...color changing flower involves two basic components: the pigments (anthocyanins), and the plant circadian...associated intellectual property concerns – patents and licensing agreements. Developing a product without the ... the rewards are great if you take the plunge – plants are the foundation of life on earth, and opportunities...feeds, foods, and fibers. And because working with plants can be challenging, there are a lot of unexplored...the complicated and unique metabolic processes of plants are being investigated on limited funding and time...also identified and characterized mechanisms and components of the circadian rhythm in petunias. Because ...before us, it’s possible for us to combine these components to develop flowers that change color. Without...

...vacuolar) pH environments that inactivate SEP and leave only DsRed to fluoresce (1). With variants that can...facilitate innovative cancer research, the Reference Reagents Group of the NCI RAS Initiative, led by Dominic...method, which allows for easy assembly of DNA fragments by utilizing regions of sequence homology (usually...and test constructs composed of complex genetic elements. To demonstrate the usefulness of GAMP, the Jacks...promoters expressing GFP, tetracycline response elements, and shRNAs many of which are available at Addgene...allows for easy and rapid targeting of complex elements to specific genomic sites. For instance, the authors... community. If you plan to use GMAP for your experiments, think about depositing your new plasmids with...