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We narrowed to 599 results for: REV;

Showing: 141 - 150 of 599 results

  1. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ... such challenges involves using knowledge from previous consulting experiences, educational training, ...productivity, and many have mechanisms in place to prevent the burden from getting overwhelming. These mechanisms...teams are facing a crunch, and using lessons from previous cases to better staff future teams. As with any...how much my scientific background, training in reviewing literature, and critical analyses skills were ...

  2. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...In a previous Plasmids 101 blog, we reviewed the salient features of several popular strains of E. coli...over-expressing a foreign protein in E. coli. We will review the potential pitfalls of recombinant protein expression... problem for toxic protein products, which can prevent the culture from reaching the desired density within...

  3. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ... But conflict can also be good. In science, a reviewer’s critical comments on your manuscript is a form.... As you perform the experiments to address a reviewer’s concerns, you are making your hypothesis stronger...majority of conflicts I address in my current role revolve around missed tasks, stalled projects, and disagreements...particular teams at that given time. I think about how previous iterations of those teams resolved similar issues...

  4. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...their publications or as a review on a company website. It is important to review the data and protocols ...Uhlen, et al. 2016 is a good place to start.) Reviewing available antibodies can seem overwhelming, especially...available resources and give yourself time to carefully review your options. If possible, try to select a couple...

  5. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...have higher apparent cellular brightness than previously reported widely used dimeric iRFPs (Table 1)....protein synthesis, degradation, or reconstitution (reviewed in Shcherbakova et al., 2018). For detection of...have lower background and higher contrast than previously developed NIR split probes (Tchekanda et al.,...near-infrared calcium indicators in this recent review (Shcherbakova et al., 2021).   Table 1: Properties...

  6. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...environment. We’ve covered five model organisms in a previous blog post, but here are five more model organisms...conserved across species, studying the axolotl may reveal the potential humans have for a similar degree ... the sequencing of the entire axolotl genome, revealing an abundance of genes related to limb regeneration...Model for Regeneration and Aging Research: A Mini-Review. Gerontology, 57(6), 565–571. https://doi.org/10.1159...

  7. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...unlikely to be found in your gene of interest) and prevent incorporation of extensive sequences used for cloning...Translation of the standardized leader peptide prevents secondary structures in the mRNA encoding your...genes from a single transcript, or if you want to prevent read-thru transcription from resulting in the production...Adaptation of Enterococcus faecalis to daptomycin reveals an ordered progression to resistance." Antimicrobial...

  8. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ... the HIV-infected reservoir, researchers have previously used histone deacetylase (HDAC) inhibitors to...the HIV-1 genome from every infected cell and 2. prevent reinfection of those cells. Kaminski et al. designed...HIV-1 cleavage allows T-cells to survive, but preventing re-infection would require sustained expression...infects over 250 million people worldwide. As we’ve previously seen in the CRISPR field, the lessons learned...

  9. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...modification was impossible. With the genome engineering revolution came epigenome-engineering tools - zinc finger...silent, they could identify the target genes of previously uncharacterized enhancers. The popular TALEN-...CRISPR activators and repressors are thought to be reversible once the effector is inactivated/removed from...KRAB induced silencing was transient and quickly reversed in culture. However, DNMT3A-induced methylation...

  10. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ... activation and downstream signaling cascades (reviewed in Abreu & Levitz, 2020), including light-sensitive...the LOV domain blocks the enzyme binding domain preventing downstream signaling pathways. Light exposure...optimize the intensities of light sources used to prevent activation of both tools with each light source....  Finding the right information When using a previously published system in your own work, you’ll need...
Showing: 141 - 150 of 599 results