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We narrowed to 466 results for: SHI

Showing: 141 - 150 of 466 results
  1. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...01039-7 Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., ...disrupting its canonical structure and Cas9 binding (Nishimasu et al., 2014). Figure 3: pegRNAs whose...
  2. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...Similarly, if you want to knock out a gene, a frameshift mutation introduced anywhere within the early...with NG PAM compatibility, as the name implies (Nishimasu et. al, 2018). xCas9 was generated by the Liu ...10.1126/science.aba8853 (Plasmids available here)   Nishimasu, H., et al.,Engineered CRISPR-Cas9 nuclease with...
  3. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...all of your plasmid data, we’ll send you prepaid shipping materials (i.e., a deposit kit) with instructions...we need to know your Addgene account username. Shipping address and phone number. Plasmid sequences or...deposit kit with instructions on how to prepare and ship the samples to Addgene and contact your technology...
  4. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...– $30,000 for research and another $30,000 in mentorship and business training. The money is a drop in...thinking about making the switch from academia to entrepreneurship, share your thoughts with us in the comments...guest bloggers! Nikolai Braun grew up outside of Washington D.C. and has always enjoyed exploring the outdoors...
  5. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...environment sooner rather than later can be helpful. Publishing: As a postdoc at AstraZeneca, you are expected...vary depending on which company you choose. If publishing is important to you, make sure that your project...an industry setting. Scientific community and mentorship: As an industry postdoc, you might find yourself...
  6. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ... situation where one wants to understand the relationship between the age of a cell, protein, or cellular...: 1585-1588. PubMed PMID: 11090358. 2. Tsuboi, Takashi, et al. "Age-dependent preferential dense-core ... 118-126. PubMed PMID: 19136976. 5. Miyatsuka, Takeshi, Zhongmei Li, and Michael S. German. "Chronology...
  7. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...construct targeting vectors. Addgene depositor Takashi Yamamoto’s lab has harnessed MMEJ to create a new...NHEJ joins the ends of a DSB in an error-prone fashion, with insertions and deletions common. In contrast...possibilities MMEJ can enable. References 1. Sakuma, Tatsushi, et al. (2016).  “MMEJ-assisted gene knock-in ...
  8. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...and Challenges CUREs inspire students to take ownership of their work and become more actively involved...Biology Department at Keene State College in New Hampshire. He mentors undergraduates conducting NIH- and...):ar55. Ma, Y., Mukherjee, S., Uzzi, B. 2020. Mentorship and protégé success in STEM fields. Proceedings...
  9. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...equally effective are shipped to customers on ice, preventing any temperature shifts. Of course, we are ...Wang et. al., 2018). Aggregation can arise from a shift in temperature, pH, or salt concentration (Amin ...
Showing: 141 - 150 of 466 results