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We narrowed to 803 results for: TIM

Showing: 281 - 300 of 803 results

  1. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    Published
    Sept. 25, 2018, 2:39 p.m.
    ...plasmid and how much time Addgene has saved researchers. Assuming 5 hours of hands-on time to make each plasmid...others to ensure that everything will ship out in time - from assembling the boxes, to inoculating tubes...scale reagent sharing led us to calculate how much time we have saved researchers. In a recent blog post...

  2. Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana

    Type
    Blog Post
    Published
    Dec. 20, 2016, 3:30 p.m.
    ...Newsletter and recently visited Cambridge to spend some time learning and doing research at Novartis. Listen ...scientists Click here to listen! Podcast breakdown (Time : Topic) 0:00 - 7:11 : Introduction - Ghanaian scientist's... opportunities in Ghana. 46:35 - end : Kwabena's time in Boston - Developing microbiome expertise. Many...Many thanks to Kwabena Duedu for taking the time to sit down and talk with us.  Kwabena Duedu, PhD is ...

  3. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...serotypes may perform better (3). Brain Region  Optimal Serotype Striatum AAV5>AAV9>AAV8>AAV1>AAV6>AAV2...outlined in the table below. Cell Type Cortex Optimal Serotype Oligodendrocytes AAV8 > AAV2 & AAV6 ...quickly. 3. Patience Is Bitter, but Its Fruit Is Sweet Time If you require a quick onset of expression, AAV ...see that gene expression really does not take much time at all. Much of the previous literature using AAVs...3 days vs 7 days vs 14 days etc. To decrease the time until expression, it is possible to use AAV constructs...

  4. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    Published
    Oct. 6, 2016, 2:30 p.m.
    ...they've managed to keep themselves happy during their time in grad school. As you'll learn, grad school has...topics. Listen to Part 1 Here Listen to Part 2 here! Time : Topic 0:00 - 0:50 : Intro 0:51 - 3:08 : The importance...right that illustrate a grad student's happiness v time throughout graduate school. This is, of course, ...We'd like to thank Niroshi and Ben for taking the time to sit down and talk to us and wish them the best...

  5. Fueled by Coffee at #SfN14

    Type
    Blog Post
    Published
    Nov. 18, 2014, 7:15 p.m.
    ...Though, to be fair, I think this is a conservative estimate. As a graduate student, I drank 3-4 cups of coffee...hear that we've been able to help their labs save time and resources by storing and distributing their ...about making such tools open-access. Though it is time for me to refill my large coffee cup and get back... – so if you’re running out of energy, then it’s time for a coffee run! ...

  6. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...African countries. Even that grim estimate might prove to be an underestimate. Given all the excitement and...itself that kills you, but the cytokine storm it ultimately sets off.  The virus works its way into immune...Zimmer’s coverage on the subject in the New York Times and on his blog, where he has offered to answer ...

  7. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 7, 2017, 1:58 p.m.
    ...organisms and their characteristic radiance for some time – there are records describing them dating back ...back to the first century A.D. – the first time anyone truly examined what caused this glowing phenomenon...organisms (E. coli, and later C. elegans) for the first time. After Chalfie’s work, scientists really started...fluorescent proteins with new colors Around the same time, researcher Sergey Lukyanov realized that, although...

  8. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...systems, the individual parts are reusable, saving time and money on future assemblies. If you are new to...for eukaryotes! The technology has been used to optimize protein secretion and expression in the biotech...guides, mini screening libraries, direct edit optimization, and more. While there are many CRISPR-Cas libraries...parts can be used in a variety of organisms, saving time and money for labs with diverse applications. The...already in place. MoClo is a fantastic way to save time and money on studies that require many assemblies...

  9. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...to as “Epi” illumination. Therefore these are sometimes call Epi-fluorescence microscopes. (See left image...sample. As more than one hole is illuminated at a time, the image can be acquired more quickly. 2-Photon...single focal plane of the sample is excited at one time, limiting photobleaching. Similar to widefield, ..., the entire field of view is excited at one time and captured in a single camera exposure. This is much...technology and parallelization (7) have allowed for real-time monitoring of neuronal activity across substantial...

  10. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    Published
    Sept. 9, 2016, 2:43 p.m.
    ...importantly, there’s the opportunity cost of your time; an investment of months of your life that may end...and the assay are just as critical for success. Sometimes, you might have a few positive control genes on...way to do something is to do it huge, there are times when a more incremental approach is prudent. What's...      References: 1. Doench, John G., et al. "Optimized sgRNA design to maximize activity and minimize...

  11. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ...use our libraries to do things - sometimes drug screens and sometimes totally different experiments we... others is that, in a relatively short amount of time, cancers become resistant to drugs, particularly...is that if you introduce them into cells one at a time, you could identify those that confer resistance...

  12. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    Published
    May 17, 2016, 2:30 p.m.
    ...and Nicole Waxmonsky Resource sharing shortens the time needed to go from planning an experiment to performing...using one of these validated gRNAs can save you the time that would be spent making and testing entirely ...CRISPR experiment.  The selection process is often time consuming and there is no guarantee that the selected...demonstrated to work in your system, it is worth spending time sequencing the genomic target to determine whether...

  13. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...skill,  time for which most researchers wouldn't have. They are motivated to get data, publish in time, defend... of art to me… The problem is that scientists sometimes forget how to communicate about their work with...its stunning images that is has been featured in Time magazine . Other smaller competitions are now taking...

  14. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    Published
    Jan. 2, 2014, 3:44 p.m.
    ...Welcome to 2014! Get excited, it’s a new year. A time for resolutions: to wake up and get to the lab earlier...been slower to adapt, likely due to the necessary time and resource investment required to change the status...’ll be able to make updates to protocols in real time. You may also want to consider moving your paper...added or removed samples. Once you've taken some time to get organized, stop back and let us know what...

  15. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ...account for three years and have only tweeted 6 times: #fail. I’d hoped to use Twitter professionally ...scientists tweet URLs with scientific domains <10% of the time. Figure 3: Top 20 Domains Tweeted by Scientists... contain scientific domains between 0-10% of the time; however, ~20% of life scientists  (biologists, ...ecologists) tweet scientific domains 20% or more of the time (red box). 8. Scientists tend to follow other scientists...

  16. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ... eight times faster than GRABNE and deactivated three times faster. This enhanced response time allows...generating a measurable signal after electrical stimulation with a single pulse, while nLightR gave a measurable...

  17. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ... stage, before you order.  Timeline Once your order is placed, we estimate it will take 6-8 weeks for ...and QC assays to be completed and reviewed. The timeline therefore will be as follows:  Request review:...Prep and QC completed: 6–8 weeks Average shipping time: 1–2 days (USA); 3–4 days (international; varies...

  18. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ...took the time to answer our questions.   Advice for success in academia How can we shape our time in academia...passion. “Love what you are doing!” It helps when the time comes to work with full force. George Church 1....during their studies. They suggest that you make time for extracurricular activities both to develop new...

  19. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...exceeding a kit’s capacity can actually reduce your ultimate plasmid yield.  Take your average column-based...available for plasmid purification. Kits can be optimized based on the plasmid size, cell model, or overall...size. Some manufacturers offer kits specifically optimized for purification of these large plasmids, while...and for extracting RNA! (That’s a blog for another time, however!) Where can you look for help?  All manufacturers...Different Quantities, Different Uses Plasmids 101: Optimizing Plasmid Yields Bringing Sustainable Practices...

  20. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    Published
    June 24, 2025, 1:15 p.m.
    ...We all know the timeline for publishing research papers can be long and unpredictable. Fortunately, you...until the article is published. This policy gives us time to complete necessary quality control and legal ...distribution, and we’ve found that this step was sometimes unclear or missed altogether. Emailing Addgene...citation to the final journal article version any time later. We recognize that a preprint isn’t necessarily...
Showing: 281 - 300 of 803 results