...Assembly has declared 2024 the International Year of Camelids. The declaration is intended to raise awareness...populations around the world. Here at Addgene, we love camelids too, though not just for the reasons the UN describes... 12–15 kDa Ease of cloning and expression from plasmids, due to only needing one open reading frame Ability...generate and more widely adopted. So thank you to the camelids who inspired this versatile family of tools, the...Membrane Proteins More resources from addgene.org  Plasmids encoding nanobodies at Addgene Sybody Generation...

...effects.  Bryan Roth’s lab created a collection of plasmids called TRUPATH to interrogate 14 of the 16 non-visual...the TRUPATH system, cells are transfected with plasmids expressing the GPCR of interest, a Gα-Rluc8 plasmid...TRUPATH be used for? The Roth lab used the TRUPATH plasmids in a number of different experimental approaches...drugs to further understand their mechanism. These plasmids are available as a collection, facilitating screening...Addgene.org Browse the catalog of fluorescent protein plasmids Find biosensors for your experiments ...

....12137. PubMed. Find the Plasmids at Addgene Kowarz Lab plasmids Izsvak Lab plasmids  ...The use of non-viral vectors, like transposons, avoids many, though not all, of these defenses. For some...for insertion in the correct orientation. These plasmids use a novel synthetic RPBSA promoter, which is...

...resource: Recombinant Antibodies! In addition to plasmids and viral vectors, you’ll now be able to order...will have unique Research Resource Identifiers (RRIDs) for each antibody, making it easy for you to determine...other recombinant affinity reagents, share your plasmids via Addgene! Email [email protected] to learn...support, we have enabled scientists to easily share plasmids with each other. Together, we can transform the.../elife.43322 Bandrowski, AE, Martone, ME (2016) RRIDs: A Simple Step toward Improving Reproducibility ...

...Addgene.org Find plasmids in Addgene's COVID-19 collection Find SARS-COV-2 pseudotype virus plasmids from Addgene...variants. “We created a series of spike expressing plasmids which encode spikes found on all of the different...response. The Balazs lab was quick to deposit their plasmids at Addgene to accelerate ongoing COVID-19 research...research. “I hope that by sharing the plasmids other scientists would be able to expand upon the work and...

...AraC and other promoter systems, head over to our Plasmids 101: Inducible Promoters article.) Modifying AraC... and shared with Addgene. You can find all the plasmids deposited from Di Ventura's lab, including the...nuclear export system (LEXY) here. Optogenetic plasmids from the Khammash lab can also be found here. ...Learn more about inducible promoters Visit the Plasmids topic overview page Resources on Addgene.org ...Molecular Biology Reference Download Addgene's Plasmids 101 eBook ...

...depositing your plasmids encoding antibodies, ScFvs, and nanobodies to Addgene! The plasmids will be made...working with the community to build a resource of plasmids encoding recombinant antibodies as well as other...through distributions of research materials, such as plasmids and viral vectors. However, it typically takes...Resources on Addgene.org Find recombinant antibody plasmids at Addgene ...

...homology-directed repair when a template is supplied. Find Plasmids from the NgAgo Publication at Addgene Cas9 and...editing efficiency. Smaller size At only 887 amino acids in length, NgAgo is shorter than SpCas9 (1368 aa.... Epub 2016 May 2. PubMed PMID: 27136078. Find plasmids from this publication at Addgene. 2. Swarts, Daan...24531762. PubMed Central PMCID: PMC4697943. Find plasmids from this publication at Addgene. 3. Swarts, ...DNA-guided nuclease that targets cognate DNA.” Nucleic Acids Res. 43(10) (2015):5120-9. PubMed PMID: 25925567...

...Looking for Optogenetics Plasmids? Plasmids for Optogenetics Research (Addgene) Plasmids from Ed Boyden's lab...conditions. Addgene: You've contributed various plasmids to Addgene over the years, many of them very popular... lab Sign up to receive email alerts when new plasmids from the Boyden lab become available. Visit Ed...

... are between 5-8 amino acids long, with a typical length of 5 or 6 amino acids (Cruse et al., 2004). Because...Antibodies may recognize a linearized sequence of amino acids, or only in their 3D conformation. Types of antibodies...sensitivity, for example by changing certain amino acids to improve its stability. Additionally, the choice...nanobodies, or synbodies, can be produced from plasmids, saving time and money. Nanobodies and synbodies...Addgene’s Antibody Plasmid Collection includes plasmids that express antibodies, nanobodies, and ScFvs...

...protein biosensors Browse Plasmids from the Tsien lab Browse the Michael Davidson collection ...The late Michael Davidson and Roger Tsien played enormous roles in fluorescent protein development by...To honor their legacy, we launched the Michael Davidson and Roger Tsien Commemorative Travel Awards in...announce the three winners of this year’s Michael Davidson and Roger Tsien Commemorative Travel Awards: Melissa...

...going on there!).  Find the Krogan Lab SARS-CoV-2 plasmids (Note: The Nsp6 construct is undergoing QC and... has been inundated with requests to share our plasmids with other researchers. We have been excited to...Gordon) have been quite busy shipping and sending plasmids to all who request them -- in locations all over...Facebook. Find more COVID-19 and coronavirus related plasmids and resources at Addgene* Many thanks to our...people, not just scientists! *If you are requesting plasmids for coronavirus or COVID-19 research, please email...

...Protein Biosensors Browse Plasmids from the Tsien Lab Browse the Michael Davidson Collection ...fluorescent proteins everyday.  The late Michael Davidson and Roger Tsien played enormous roles in enabling...their legacy, we recently launched the Michael Davidson and Roger Tsien Commemorative Travel Awards. These... we’re proud to announce our first two Michael Davidson and Roger Tsien Commemorative Travel Awardees:...

...Read More: All Plasmids 101 Articles Browse plasmids that contain luciferase ...choose from) is now a common feature in reporter plasmids.  Learn How Luciferase Can Be Used In Concert ...hirtus) 630 64 Luciferin ATP No Common uses for plasmids expressing luciferase Luciferase reporter assay... plasmids 101...

...largest new initiative since we started distributing plasmids in 2004. We’ve already distributed over 500 viral...depositors. Over 3,000 laboratories are sharing plasmids (with 16 labs currently sharing viral preps) through...positive response to the option of Addgene providing plasmids in a ready-to-use viral format. Having identified.... With their expertise in shipping hundreds of plasmids a day, the Lab and Office Teams develop shipping...institutions to distribute viral preps derived from plasmids in the repository. The Outreach Team has been ...

...efficiency. In the same vein, locked nucleic acids or bridged nucleic acids can also be introduced in the gRNA....consider include amide linkages, unlocked nucleic acids, and a constrained ethyl.  Can’t decide which RNA...While both have similar advantages, bridged nucleic acids with N-methyl substitutions are slightly more efficient...ready-to-use viral preps of select CRISPR lentiviral plasmids Additional resources on the Addgene Blog  How...

...24360272. PubMed Central PMCID: PMC3918502. Find plasmids from this publication at Addgene. 2. Ma, Hanhui...25713381. PubMed Central PMCID: PMC4364232. Find the plasmids from this publication at Addgene. 3. Ma, Hanhui...27088723. PubMed Central PMCID: PMC4864854. Find the plasmids from this publication at Addgene. 4. Kiani, Samira...26344044. PubMed Central PMCID: PMC4666719. Find plasmids from this publication at Addgene. Additional ...Resources on Asddgene.org Find Fluorescent Protein Plasmids Find Fluorescent Protein Kits Find Empty Backbones...

...Split-BioID plasmids Find CRISPR plasmids for labeling genomic regions Find CRISPR plasmids for purifying...question that RaPID could help answer? The RaPID plasmids are available from Addgene! References Ramanathan...PubMed Central PMCID: PMC5886736. Find the RaPID Plasmids Here! McHugh, C.A., Russell, P., & Guttman, M...

... specific antibody to the C-terminal five amino acids, meaning the tag could be quite small. However, ...epitope recognized by Myc1-9E10 down to 10 amino acids (EQKLISEEDL) - larger than the substance P peptide...., who published a paper showing that the amino acids neighboring the Myc epitope influence 9E10’s affinity...Addgene blog Antibodies 101: Monoclonal Antibodies Plasmids 101: Peptide Tagging Plasmid-based Recombinant... recombinant anti-c-Myc [9E10] at Addgene Find plasmids with the myc-tag  ...

...More about Plasmids for Endogenous Gene Tagging in Human Cells Check Out the Michael Davidson Fluorescent... on the gene names in the figure below to find plasmids containing that particular gene. ...Prescott (Monash University). You can find more plasmids for labeling your subcellular structure of interest... for Cell Science Plasmid Collection Check Out Plasmids for FRET Check Out the Fluorescent Protein Guide...