We narrowed to 513 results for: EMB

1. Antibodies 101: Affinity Reagents

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   Blog Post

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   April 14, 2022, 1:15 p.m.

   ...through mutation, allowing diabodies to be used for assembling protein nanostructures. Diabodies readily co-...

2. Transferable Skills Guide: Problem Solving

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   Blog Post

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   Nov. 30, 2021, 2:15 p.m.

   ...both in and out of the lab. The next step is to remember that you have these skills when you start applying...

3. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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   Blog Post

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   Aug. 5, 2021, 12:15 p.m.

   ...multiplexed imaging of neurotransmitter, calcium, and membrane voltage,” says Tian. Figure 5: Citrine-tagged...

4. Viral Vectors 101: An Introduction to AAV

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   Blog Post

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   May 30, 2023, 1:15 p.m.

   ...expression for several years (Wonjo et al., 2013). Remember that AAVs do not integrate into the host genome...

5. The time and cost required to make a plasmid

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   Blog Post

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   March 23, 2023, 1:15 p.m.

   ... is a concern. The faster methods, like Gibson assembly, are typically more expensive, so you’ll have ...

6. Gendered Innovations: Why Does Sex of the Cell Matter?

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   Blog Post

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   July 21, 2016, 2:30 p.m.

   ...analysis for innovation and discovery. She is a member of the American Academy of Arts and Sciences and...

7. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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   Blog Post

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   Dec. 16, 2015, 3:30 p.m.

   ... their ability to cut the EMX1 locus in human embryonic kidney (HEK) cells, 11 of these initial mutants...

8. The Future of Research Symposium Boston 2015

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   Blog Post

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   Oct. 20, 2015, 2:30 p.m.

   ...posing some of their own are the following panel members: Jonathan Dinman, Chair of the Cell Biology and...

9. New Tool for Lineage Tracing: The ClonTracer Library

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   Blog Post

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   Sept. 22, 2015, 2:30 p.m.

   ...library allows a researcher to label individual members of a starting population of cells with specifc,...

10. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    Blog Post

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    Nov. 14, 2024, 2:15 p.m.

    ...splice sites. Nat Biotechnol. Published online November 5, 2018. doi:10.1038/nbt.4283 Zibitt MS, Hartford...

11. Engaging with science and society at pgEd

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    Blog Post

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    Sept. 20, 2018, 12:17 p.m.

    ...’ve since returned to pgEd as a full-time staff member for the past three years. pgEd is a small team ...

12. Overcoming the Challenges of Lentiviral Production

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    Blog Post

    Published

    Aug. 7, 2018, 12:31 p.m.

    ...dish can be easily applied to multiple dishes, remember that handling multiple dishes will take longer...

13. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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    Blog Post

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    June 26, 2018, 1:33 p.m.

    ...s research is the mechanisms by which proteins assemble into functional multi-subunit macromolecular complexes...

14. Adenoviral Vector Production and Troubleshooting

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    Blog Post

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    June 14, 2018, 11:44 a.m.

    ...packaging signal (Ψ), both of which are essential for assembly of the virion. Adapted from Lee et al., Adenovirus-mediated...

15. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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    Blog Post

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    May 1, 2018, 1:57 p.m.

    ...considerations for Split-BioID It’s important to remember that with Split-BioID, and BioID, any protein ...

16. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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    Blog Post

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    July 23, 2020, 1:20 p.m.

    ... with Cas9 interferes and prevents the correct assembly of the crRNA-Cas complex (Zhu et al., 2019). Inhibiting...

17. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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    Blog Post

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    June 25, 2020, 1:15 p.m.

    ...Addgene as a DNA Sequencing Analyst intern. As a member of the Quality Control team, I help make sure the...

18. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post

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    April 6, 2017, 1:02 p.m.

    ...technique that briefly destabilizes the cell’s outer membrane and allows the components to physically enter ...

19. Corralling and Cornering the Goldilocks Antibody

    Type

    Blog Post

    Published

    Aug. 9, 2022, 1:15 p.m.

    ...Zhuhao Wu and his colleagues! Dr. Wu and his lab members (like me!) are dedicated to creating a public and...

20. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    Blog Post

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    Aug. 24, 2021, 1:15 p.m.

    ...your standard curve to plot to a linear equation (remember y = mx+b?). Figure 5: A standard curve ...

21. CRISPR in the Clinic

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    Blog Post

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    Nov. 2, 2023, 1:15 p.m.

    ... in clinical trials includes Cas3 (the shredder member of the Cas family) as part of a cocktail therapy...

22. Viral Vectors 101: Virus Safety

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    Blog Post

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    June 22, 2023, 1:15 p.m.

    ...biosafety cabinet. When decontaminating liquid waste, remember that the percentage indicates the proportion of...

23. Antibodies 101: Choosing the Right Isotype

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    Blog Post

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    April 6, 2023, 3 p.m.

    ..., G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical Techniques for Light and ...

24. 6 Tips for Grant Writing

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    Blog Post

    Published

    Oct. 18, 2016, 2:30 p.m.

    ...about what reviewers in your area are looking for. Remember though, you need to leave them time to read it...

25. Tips for Technical Support Calls

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    Blog Post

    Published

    Sept. 20, 2016, 2:39 p.m.

    ... money on a potentially faulty product. Please remember that the person at the other end of that email...

26. Common Injection Routes in Mice

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    Blog Post

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    Jan. 22, 2025, 9:42 p.m.

    ...When considering what method you’d like to use, remember that the final decision will likely come down ...

27. Pooled Library Amplifications

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    Blog Post

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    May 30, 2024, 1:30 p.m.

    ... protocols attached.  Final step: validation  Remember, there is no “one-size-fits-all” protocol! Each...

28. Antibodies 101: Flow Compensation

    Type

    Blog Post

    Published

    May 16, 2024, 1:15 p.m.

    ...But we hope this guide can help you get started. Remember, you can always change your compensation values...

29. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type

    Blog Post

    Published

    April 4, 2019, 12:53 p.m.

    ...principle, Peters and colleagues constructed a 40-member library targeting 20 genes of Enterobacter cloacae...

30. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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    Blog Post

    Published

    Feb. 4, 2020, 2:15 p.m.

    ...camouflage. An anemone’s red colored tentacles resemble worms which attract fish for them to prey upon...