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1. Educational Planarian Tools for Teaching Developmental Biology Techniques

   Type

   Blog Post

   Published

   May 24, 2018, 1:20 p.m.

   ... http://abt.ucpress.edu/content/79/3/208 2. Fire, S. Xu, M.K. Montgomery, S.A. Kostas, S.E. Driver, C....

2. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

   Type

   Blog Post

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   May 15, 2018, 12:43 p.m.

   ...and other required enzymes, including a glutathione S-transferase homolog from a Janthinobacterium sp. Moreover...

3. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

   Type

   Blog Post

   Published

   Sept. 26, 2019, 12:50 p.m.

   ...tropism of Nanoblades, different viral envelope protein(s) are expressed to pseudotype Nanoblades. The team ...

4. Hot Plasmids - October 2020

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   Blog Post

   Published

   Oct. 13, 2020, 1:15 p.m.

   ...time that were not previously possible.   Bensussen S, et al., iScience 2020. https://doi.org/10.1016/j....

5. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

   Type

   Blog Post

   Published

   May 1, 2018, 1:57 p.m.

   ...3308701. Find the BioID Plasmids Here! Macneill, S., & Varnaitė, R. (2016). Meet the neighbors: Mapping...

6. Progress Towards a PAM-Free CRISPR

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   Blog Post

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   March 7, 2024, 2:39 p.m.

   ... Resources References Kwart, D., Paquet, D., Teo, S. et al. Precise and efficient scarless genome editing...

7. Antibodies 101: Conventional vs Spectral Flow Cytometry

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   Blog Post

   Published

   Aug. 3, 2023, 1:15 p.m.

   ...overlapping markers. Compensation/reference control(s) Compensation or “reference” controls are samples ...

8. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

   Type

   Blog Post

   Published

   Dec. 14, 2021, 2:15 p.m.

   ...https://doi.org/10.1016/j.neuron.2013.08.032.  Morita S, Noguchi H, Horii T et al. (2016) Targeted DNA demethylation...

9. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

   Type

   Blog Post

   Published

   April 6, 2021, 12:30 p.m.

   ...//doi.org/10.1089/hum.2019.277 Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD...

10. A Look at Addgene's QC Process

    Type

    Blog Post

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    May 6, 2025, 1:15 p.m.

    ... the de novo NGS result to the deposited sequence(s) in SnapGene. SnapGene annotates the plasmid maps ...

11. 3D Printing Meets CRISPR Cas9

    Type

    Blog Post

    Published

    Oct. 17, 2017, 12:55 p.m.

    ...interactive images of their proteins. Since the 1980’s, journal articles have been filled with impressive...

12. Fluorescent Proteins 101: Introduction to FRET

    Type

    Blog Post

    Published

    June 27, 2017, 1:03 p.m.

    ...in drug discovery. References 1. Baird, Geoffrey S., et al. "Circular permutation and receptor insertion...

13. Tips for Technical Support Calls

    Type

    Blog Post

    Published

    Sept. 20, 2016, 2:39 p.m.

    ... also let us know! Sometimes those few “thank you”s are what get a customer service agent through a busy...

14. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type

    Blog Post

    Published

    Sept. 13, 2016, 2:30 p.m.

    ...Pubmed Central PMCID: PMC4866822. 3. McDowell, Gary S., et al. "Shaping the Future of Research: a perspective...

15. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type

    Blog Post

    Published

    May 22, 2015, 4:58 p.m.

    ...science.aaa5945. PubMed. DiCarlo, J. E., Chavez, A., Dietz, S. L., Esvelt, K. M. & Church, G. M. RNA-guided gene...

16. 15 Hot Plasmids from 2017

    Type

    Blog Post

    Published

    Jan. 9, 2018, 3:02 p.m.

    ...their useful reagents shared via Addgene. Narayan S, et al. Cell Reports. 2017. PubMed PMID: 28813671 ...the popular type II CRISPR systems, which include S. pyogenes (Spy) Cas9, been identified. In Rauch, et...

17. Summer SciComm Series: Modes of Communication

    Type

    Blog Post

    Published

    July 1, 2022, 1:15 p.m.

    ...following four questions to figure out which method(s) work best for you, your audience, and your science...

18. Pooled Library Amplifications

    Type

    Blog Post

    Published

    May 30, 2024, 1:30 p.m.

    ...and how many cells to use for your transformation(s). The amount of competent cells you need total and...

19. Twenty Years of Addgene Sharing: CRISPR

    Type

    Blog Post

    Published

    April 18, 2024, 1:15 p.m.

    ...References and resources References Pyhtila, B., Kasowitz, S., Leeson, R., & Barrangou, R. (2023). The Expanding...

20. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type

    Blog Post

    Published

    April 4, 2019, 12:53 p.m.

    ...in several bacteria associated with human disease (S. aureus, Klebsiella pneumoniae, and P. aeruginosa)...