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We narrowed to 574 results for: BASE;

Showing: 281 - 300 of 574 results

  1. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...barcoded gRNA library is now ready for CombiGEM-based assembly into the CombiGEM lentiviral vector backbone...: PMC4420636. 7. Ma, Hongming, et al. "A CRISPR-based screen identifies genes essential for West-Nile-...

  2. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    Published
    Aug. 5, 2025, 1:15 p.m.
    ...conclusions. Antibody engineering also benefits antibody-based therapeutics. Whether antibodies are used directly...measurement of antigen-antibody interactions in a plate-based format, making it suitable for comparing relative...biology, and protein engineering to develop antibody-based cancer therapeutics.    References and Resources...

  3. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ... lentiGuide-based) Mammalian Lentivirus pRDA_722 (CMV-driven; lentiCRISPRv2-based; preferred ...

  4. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...expand your search. Results in your feed are ranked based on their influence on the network, or body of literature...library. The library will suggest additional papers based on the papers you have saved. This library can also...

  5. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...this limitation, we created a generalizable tag-based strategy, which we refer to as the degradation tag...system is versatile and can be used in both cell-based assays and mouse models. The dTAG system The dTAG...

  6. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...specificity. Strength and accuracy The strength is based on binding specificity and exposure time. We first...: ValidationSelecting the Right AntibodyPlasmid-based Recombinant Antibodies    ... dedicated to creating a public and accessible database for low-cost, high-quality antibodies for IHC....reference. The Gift of Giving As an open-source database, the mAb3D Atlas will foster collaboration, progress...reliable, high-quality antibodies. We are creating a database for goldilocks antibodies and, alongside that,...

  7. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...Nanoblades are a Cas9 RNP delivery vehicle that’s based on mouse leukemia virus (MLV) virus-like particles...Homology directed repair (HDR) with Nanoblades CRISPR-based HDR allows for the precise insertion of a DNA sequence...

  8. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...mammalian cells. Despite the popularity of CRISPR-based knockout/knock-in systems, these systems remain ...difficult. Addgene depositors have developed new CRISPR-based methods for various knock-ins, discussed in our ...

  9. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...your email or phone call. Though my experience is based solely on my time at Addgene, I’m confident that...minutes from our phone call. If your concern is based on data you have produced, please share the data...

  10. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...illustrious speaker list attracted a host of sponsors based all over the world. With three Nobel Prize winners...hypothesis that these pathways are involved in RNA based surveillance of repetitive gene families and provide...

  11. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...or repressor domains to control gene expression. Base editors can help you make precise point mutations...consider the best form for those CRISPR components based on your model system. CRISPR reagents can be delivered...

  12. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...photos/72005145@N00/5600978712/ What CRISPR-based reagents are available for genome-wide screens? ...enriched using either drug selection or fluorescence-based cell sorting and screened for a particular phenotype...

  16. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...is vital for the accurate interpretation of FRET-based experiments. A popular combination is Alexa594, ...dyes, expanding the capabilities of fluorescence-based techniques in biotechnology. Recent examples of ...2016). Development of a quantitative fluorescence-based ligand-binding assay. Scientific Reports, 6. https...

  17. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...event, and assigns each event to a channel number based on intensity. Higher intensity events are assigned...to the protein in its native state.  Antibodies-based flow cytometry can use both direct and indirect ... background information for setting up antibody based flow cytometry experiments.       References and...

  18. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...questions, which are critical in setting up a FRET-based assay or screen (the Addgene blog has an excellent...different city, you can still develop a successful FRET-based assay since energy transfer only occurs when donor...10. Saraheimo, Satu, et al. "Time-Resolved FRET-Based Approach for Antibody Detection–A New Serodiagnostic...

  19. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ... electroporations. The specifics of this can be based on the manufacturer’s protocol for transformation...transformation efficiency, or more specifically calculated based on the size of and weight of your library plasmids...

  20. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ... different categories based on function: knockout (KO), activate, inhibit, base edit, and other. The first...
Showing: 281 - 300 of 574 results