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We narrowed to 582 results for: BASE;

Showing: 281 - 300 of 582 results
  1. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...and sensitive enough to detect gene copy number based on the number of fluorescent puncta observed. By...Addgene Blog Read More about Fluorescent Protein-Based Tools Learn Some of the Pitfalls When Using Fluorescent...
  2. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...learn how to communicate their work differently based on their audience. How you talk about your experiments...problem was the creation of an Addgene lexicon. The base lexicon was and continues to be a list of commonly...
  3. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...the Rare Genomics Institute by providing plasmid-based tools for rare disease research. Congratulations...events, we will utilize BRET-, FRET-, and intensity-based signaling biosensors to monitor intracellular signaling...
  4. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...the concentration of proteins in an ELISA well is based on a colorimetric reaction. Most commonly, this ...concentration of protein in the experimental wells based on the equation of the line. With these tips in ...
  5. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
    Published
    Oct. 21, 2025, 1:15 p.m.
    .... Using the natural amino acids or nucleic acid bases already present throughout living organisms, Click... CLIP-tags Tetbow SNAP-tag, CLIP-tag, HaloTag Based on the Brainbow system, adds Tetracycline (Tet) ...
  6. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...track cell-cell interactions By Jennifer Tsang GFP-based Touching Nexus (G-baToN) is a new tool developed... quantification of this depletion using imaging-based measurements (Ashley et al., 2021).   Schematic...
  7. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...barcoded gRNA library is now ready for CombiGEM-based assembly into the CombiGEM lentiviral vector backbone...: PMC4420636. 7. Ma, Hongming, et al. "A CRISPR-based screen identifies genes essential for West-Nile-...
  8. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    Published
    Aug. 5, 2025, 1:15 p.m.
    ...conclusions. Antibody engineering also benefits antibody-based therapeutics. Whether antibodies are used directly...measurement of antigen-antibody interactions in a plate-based format, making it suitable for comparing relative...biology, and protein engineering to develop antibody-based cancer therapeutics.    References and Resources...
  9. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...this limitation, we created a generalizable tag-based strategy, which we refer to as the degradation tag...system is versatile and can be used in both cell-based assays and mouse models. The dTAG system The dTAG...
  10. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...Nanoblades are a Cas9 RNP delivery vehicle that’s based on mouse leukemia virus (MLV) virus-like particles...Homology directed repair (HDR) with Nanoblades CRISPR-based HDR allows for the precise insertion of a DNA sequence...
  11. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...specificity. Strength and accuracy The strength is based on binding specificity and exposure time. We first...: ValidationSelecting the Right AntibodyPlasmid-based Recombinant Antibodies    ... dedicated to creating a public and accessible database for low-cost, high-quality antibodies for IHC....reference. The Gift of Giving As an open-source database, the mAb3D Atlas will foster collaboration, progress...reliable, high-quality antibodies. We are creating a database for goldilocks antibodies and, alongside that,...
  12. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ... lentiGuide-based) Mammalian Lentivirus pRDA_722 (CMV-driven; lentiCRISPRv2-based; preferred ...
  13. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...expand your search. Results in your feed are ranked based on their influence on the network, or body of literature...library. The library will suggest additional papers based on the papers you have saved. This library can also...
  14. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...mammalian cells. Despite the popularity of CRISPR-based knockout/knock-in systems, these systems remain ...difficult. Addgene depositors have developed new CRISPR-based methods for various knock-ins, discussed in our ...
  15. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...your email or phone call. Though my experience is based solely on my time at Addgene, I’m confident that...minutes from our phone call. If your concern is based on data you have produced, please share the data...
  16. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...illustrious speaker list attracted a host of sponsors based all over the world. With three Nobel Prize winners...hypothesis that these pathways are involved in RNA based surveillance of repetitive gene families and provide...
  17. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...or repressor domains to control gene expression. Base editors can help you make precise point mutations...consider the best form for those CRISPR components based on your model system. CRISPR reagents can be delivered...
  18. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...photos/72005145@N00/5600978712/ What CRISPR-based reagents are available for genome-wide screens? ...enriched using either drug selection or fluorescence-based cell sorting and screened for a particular phenotype...
Showing: 281 - 300 of 582 results