We narrowed to 322 results for: BRE

1. Viral Vectors 101: Preparing Pooled Libraries

   Type

   Blog Post

   Published

   July 13, 2023, 1:15 p.m.

   ...incubation time, antibiotic conditions, and amount of polybrene. (psst…didn’t get your library pre-made from Addgene...

2. CRISPR-based Genome Editing Systems For Editing in E. coli

   Type

   Blog Post

   Published

   April 13, 2021, 1:15 p.m.

   ...engineering single genome edits but the double strand breaks it introduces makes it difficult to perform two...

3. CRISPR 101: Any Base Transversion Editors

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   Blog Post

   Published

   April 1, 2025, 1:15 p.m.

   ...also called apurinic or apyrimidinic sites and abbreviated as AP sites. A collaborative team from the David...

4. Hot Plasmids: Fall 2024

   Type

   Blog Post

   Published

   Nov. 21, 2024, 1:30 p.m.

   .... abscessus. A) Sth1Cas9 mediates double strand break at the target site, which results in either bacterial...

5. Plasmids 101: Stringent Regulation of Replication

   Type

   Blog Post

   Published

   June 20, 2024, 1:15 p.m.

   ...strands. Created with BioRender.com. Once DnaA breaks the local hydrogen bonds holding the DNA together...

6. The PAM Requirement and Expanding CRISPR Beyond SpCas9

   Type

   Blog Post

   Published

   Aug. 20, 2020, 2:30 p.m.

   ... Cpf1), a nuclease that generates double strand breaks in target genes resulting in the formation of “...

7. Finding nucleic acids with SHERLOCK and DETECTR

   Type

   Blog Post

   Published

   April 16, 2020, 1 p.m.

   ... generates 5’ and 3’ staggered ends after dsDNA breaks. The Doudna lab discovered Cas12a’s ability to ...

8. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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   Blog Post

   Published

   Feb. 18, 2020, 2:15 p.m.

   ...matching Mentors with Singletons for short coffee breaks. While this was not the more intense mentorship...

9. An Inside Look into Addgene's Dev Team

   Type

   Blog Post

   Published

   Jan. 23, 2020, 2:45 p.m.

   ...collaborative approach to development. Scrum teams plan and break up work into small, well defined chunks, called ...

10. Antibiotic Resistance: An Old Solution but a New Problem

    Type

    Blog Post

    Published

    Nov. 10, 2016, 3:30 p.m.

    ..., we can all work together to try and prevent outbreaks of drug resistant bacteria within communities,...

11. Analyzing CRISPR Editing Results with ICE from Synthego

    Type

    Blog Post

    Published

    May 8, 2018, 1 p.m.

    ...single or multiplex CRISPR-Cas9 double-strand DNA breaks using SpCas9. More features will be added to the...

12. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type

    Blog Post

    Published

    May 2, 2019, 12:41 p.m.

    ... (Virginia Weis) Sea anemone Aiptasia - algae Breviolum minutum symbionts are important models for solutions...

13. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type

    Blog Post

    Published

    April 2, 2019, 1:44 p.m.

    ...first conference room, that is actually used as a break room for Addgenies, was named after the protein ...

14. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type

    Blog Post

    Published

    Nov. 1, 2018, 12:35 p.m.

    ...it in the cleaved complex. This results in DNA breakage and cell death. Conversely, CcdA binds to and ...

15. Deciphering the Mysteries of Behavior with Viral Vectors

    Type

    Blog Post

    Published

    Sept. 17, 2019, 12:40 p.m.

    ....12 (2007): 1079. PubMed PMID: 17700574. Hare, Brendan D., et al. "Optogenetic stimulation of medial prefrontal...

16. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type

    Blog Post

    Published

    Aug. 13, 2019, 12:58 p.m.

    ... genetic resources and highlight two recent breakthroughs in developmental biology that arose from zebrafish-based...

17. Plasmids 101: Broad Host Range Plasmids

    Type

    Blog Post

    Published

    April 11, 2024, 1:15 p.m.

    ...Alcaligenes eutrophus, Azotobacter vinelandii, Brevibacterium methylicum, Caulobacter crescentus, Desulfovibrio...

18. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type

    Blog Post

    Published

    Nov. 19, 2020, 2:15 p.m.

    ...MECP2 gene, and highlights some of the latest breakthrough therapeutic approaches. What is Rett Syndrome...

19. The Challenges of Cell Culture

    Type

    Blog Post

    Published

    May 5, 2016, 2:30 p.m.

    ...2002): 315-319. PubMed PMID: 12001993. 4. Lucey, Brendan P., Walter A. Nelson-Rees, and Grover M. Hutchins...

20. Getting the Most from Your Lentiviral Transduction

    Type

    Blog Post

    Published

    April 7, 2016, 2:30 p.m.

    ...typically employ the positively charged polycation, polybrene. Interestingly, one study found that alternative...