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  1. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ..., across four different types of human cells, including T cells and hematopoietic stem and progenitor ... from existing multiplex array architectures, including previous tRNA-gRNA arrays, that rely on an upstream...

  2. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ... new contact to connect on LinkedIn and always include a personal note about where you met and what you...can be as simple as “it was nice meeting you.” Include follow-up questions or requests for advice based...

  3. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...that can influence the HDR/NHEJ decision. Our conclusions reinforce a key scientific principle: that understanding...frequencies of genome cutting and, when donor DNA is included in the nucleofection reaction, extremely high ...

  4. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...are multiple methods of DNA assembly available, including: Gibson Assembly®, BioBrick® Assembly, Golden ...applications of the NEBuilder HiFi DNA Assembly Mix include:  Generation of constructs with multiple point...

  6. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...form concatemers in later steps. This new NEB kit includes a step that both fragments the DNA and prevents...libraries. NEB also gave us a few goodies which included an NEB drawstring backpack and a double wall insulated...

  7. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...understand and grow the scientific ecosystem, including understanding how shared materials are used to...ll be confident in what to expect. No system, including MTAs, is perfect, but it does provide consistency...

  9. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...plasmids differ from their bacterial counterparts, including how replication occurs and whether selection is...in the cell, as many of these experiments are concluded within 48 h of transfection. Of course, it is ...

  10. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...80,000+ reagents and 700+ educational resources — including blog posts, protocols, guides, and collections...we rolled out multiple changes to our website, including the recent homepage and search tool updates. Now...

  12. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...backbone to generate R-mAbs of different subclasses, including subclasses that were not produced by the original...expanded their recombinant affinity reagent tools to include nanobodies. At Addgene, we are excited to be part...

  13. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...Others ran experiments at odd hours, used more secluded workspaces, or politely asked to not be disturbed... of making an error. Try a variety of things, including (and maybe especially) suggestions you are initially...

  14. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...generate antibodies against many types of molecules including proteins, lipids, and carbohydrates, in this blog...2005). This is reflected in the antibody’s cost. Conclusion Whether you choose to use a monoclonal antibody...

  15. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...be activated by an array of different ligands, including hormones, neurotransmitters, photons or odorants... new receptors were termed Designer Receptor Exclusively Activated by Designer Drugs (DREADDs) (Roth, ...

  16. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ... DNA there, although other restriction sites (including other XbaI sites not preceded by GA or followed...either be difficult to interpret or lead you to conclude your plasmid is not correct! Conversely, enzymes...

  19. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...expression of other endonucleases, like Csy4, or the inclusion of processing signals, such at tRNAs, to process...its own pre-crRNA arrays eliminates the need to include multiple promoters to drive crRNA expression and...

  20. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...will it make them think? Does this lead to the conclusions I want them to draw and the perspective I want...scientists has formed around science communication, including the teams at Addgene, ComSciCon, and many more...
Showing: 281 - 300 of 650 results