We narrowed to 614 results for: CaS

...vectors fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors. Retroviral...allowing for selection of the transduced cells. In this case your gene and the marker can be separated by an ...rtTA) to bind TRE only in presence of Dox. In this case, your gene is repressed in steady state and is expressed...directly added to your target cells. However in that case you don’t know how many viral particles you put ...

...favorite twitter #agarart accounts, Petri dish Picasso (@PetriPicasso) came together and delivered very impressive...Colorado -Anschutz Medical Campus (@CSD_CUAMC) caught a case of holiday cheer, and we loved it! Check out their...

... In this episode of the Addgene Podcast, we introduce you to the Journal of Emerging Investigators, an...interview with the Journal of Emerging Investigators Podcast breakdown Intro Chapter 1 - Introduction to Mark...the audio engineer and producer for the Addgene Podcast.      Additional Resources from the Addgene Blog...

...a peer-reviewed manuscript OR a preprint. In the case of a preprint submission, we are happy to update...cloned into it. gRNAs are used for directing CRISPR/Cas9 to a targeted genomic location, while shRNAs are...format, e-mail us at  [email protected] Special cases Please contact us at [email protected] or +1 (...) if your deposit falls into one of the special cases below:   Pooled Library A pooled library is a ...

...with his new company, Science with Tom. In this podcast, we learn more about Tom and pick his brain for...the audio engineer and producer for the Addgene Podcast.     Additional Resources on the Addgene Blog ... a Grad Student Community Check out All of Our Podcasts Find Great Educational Content in our Plasmids...

... School researcher Connie Cepko. Listen to the podcast below to hear all about the research being conducted...interview. Happy Listening! Click here to listen! Podcast breakdown: (Time : Topic) 0:00-1:00 : Introduction...the audio engineer and producer for the Addgene Podcast.     References 1.Tang, Jonathan C.Y., et al. “...

...In this episode of the Addgene Podcast, we sit down with Kwabena Duedu, a researcher at the University... to Ghanaian scientists Click here to listen! Podcast breakdown (Time : Topic) 0:00 - 7:11 : Introduction...the audio engineer and producer for the Addgene Podcast.     Additional Resources on the Addgene Blog ...

...endogenous levels (Laflamme et al., 2019). CRISPR-Cas9 approaches are commonly used to produce KO cell ...is not amenable to knockout approaches. In these cases, we would recommend a knockdown approach instead...lines based on expression of the target. In this case, you could compare the antibody’s labeling of the...Biddle, M. S., Smallwood, D. T., Weston, C. A., Castells, E., Bowman, V. W., McCarthy, J., Amrani, Y., ...

... lentiviral transfer vector used to deliver both Cas9 and your gRNA of interest to mammalian cells. It... blog post on Genome-wide Screening Using CRISPR/Cas9. If you need help cloning a gRNA into lentiCRISPR...References 1. Shalem, Ophir, et al. "Genome-scale CRISPR-Cas9 knockout screening in human cells." Science 343.6166...

... frequently used elements in plant expression cassettes: plant promoters, N-terminal tags, coding sequences...C-terminal tags, plant terminators and resistance cassettes for selection of transgenic plants. The complete... gives the option to stack several expression cassettes onto a single T-DNA in subsequent rounds, dramatically...

...simultaneous gene activation in plants.  FnCas12a, unlike other Cas12a nucleases identified, functions at temperatures...editing in moderate thermophiles.  These evolved SpCas9 variants have improved activity on NAG PAMS and...

...at eScience Labs also write test question banks, case studies, and experiments for labs, in addition to...comprehensive exam proposals, a few PhD theses, and occasionally posting something science-related on Facebook...science education tools? Check out this Addgene podcast with educator and science rap mastermind Tom McFadden...

...Camelid family is composed of camels, llamas, and alpacas (and their undomesticated counterparts) - not exactly...soon after found similar antibodies in llamas and alpacas (Figure 1). At the time, researchers were already...started with immunizing a camelid (usually llamas or alpacas). But because sdAbs are so amenable to recombinant...

...skill acquisition. Classes covered filmmaking, podcasting, philosophy of science, creative writing, designing...an animated video depicting nuclear fission; a podcast with a genetics researcher on parasitoid wasps;...the face of disparate spheres of research. In my case, an interest in neuroscience was carried over and...

...sgRNAs to guide Cas9 to targeted genes. In these applications, however, nuclease-dead Cas9 is fused to a...libraries In CRISPR knockout screening, sgRNAs guide Cas9 to cut targeted genes, creating mutations that prevent...

...research. Image from Reproducibility for Everyone. Case study 1: Cell lines Many of the reagents used to...world shows that science is truly a global endeavor. Case study 2: Plasmids Another example of reagents that...Browse Addgene's COVID-19 plasmids and resources! Case study 3: Mouse strains Reagents developed during...

...question to ask is what to do with them. In some cases, there might be a mutation that disrupts the function...mutation in the same genetic background. In other cases, YFG or one of its mutant variants will need to ...systems exist (eg. LexA/LexAop and Cre/Lox systems) in case you want to express one gene in one cell type and...

...FAP - fluorogen activating protein - dL5** in this case), and 2) a fluorogen (the TAP - targeted and activated...activated photosensitizer - MG-2I in this case). The fluorogen is not fluorescent until it binds its protein...easy to watch development in real time.  In both cases, dL5** was tagged with mCerulean3 (mCer3) for visualization...

...unmeasurable skills and productivity coming from that casual discussion on the way to the office or in the kitchen... flexible work schedule the more we realize how casual chatter is very much missed in the virtual world...widely used and could bring back a taste of that casual kitchen chatter, ultimately sparking new ideas....

...Th2) cells from mice that constitutively express Cas9 and screen for genes that affect Th2 differentiation...Wang Lab created a tripartite system consisting of dCas9, a gRNA fused to an RNA binding motif, and a RNA-binding...protein-bEFP fusion. Thus, the bEFPs bind the UAS via the dCas9-gRNA complex which allows transcription from the...