Skip to main content
Addgene
Showing: 281 - 300 of 620 results

  1. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ...areas of research. We welcome your comments... Where do you think these technologies are going? What is the...

  3. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...and a quick zap of the proper color light, you can do just that.  Photosensitizing dyes and proteins have...than shorter wavelengths, which makes it possible to do things like in vivo imaging.   Excitation with NIR...

  4. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...experimental conditions. This is time well spent and, if you do it right, can be (part of) figure 1 of your next ...reported properties of fluorescent proteins, how to do a head-to-head comparison of fluorescent proteins...

  5. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...If you’re using CRISPR to make a genome edit, how do you know if your CRISPR experiment was successful...experiments. But how? There have been several systems to do this but most are labor-intensive, require multiple...

  6. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...fallen into studies of who’s there and what are they doing. To address these questions, biologists often use...transcripts gives clues into what the microbes are doing. But aside from sequencing, scientists can also ...

  7. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ... time and budget you cannot test them all, so how do you know which ones to try?  Read on and we will ...turn to for advice if you need to troubleshoot. What do you know about the vendor and the vendor’s technical...

  8. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...is an isotype? How many are there? Why do they matter? And how do antibodies switch isotypes? What is an...

  9. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...together long enough to get it into cells and let them do the repair work. The key is T4 DNA polymerase, which...and its low cost makes it ideal for researchers doing large amounts of cloning. Assembly is scarless, ...

  10. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...Frequently asked questions about γ-retroviral vectors How do engineered γ-retroviruses differ from lentiviruses...promoter is no longer present in these constructs. Why do many γ-retroviral plasmids have similar names? The...

  11. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ... scifi movie? Or maybe it’s a biosensor?! You can do more than just ‘sense’ biological compounds and reactions...activities.       Viruses and biosensors So how do viruses factor into all of these diverse biosensors...

  12. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...site specifically incorporated ncAA, ready for you do to any number of experiments with. The catch The...broadly useful for biomedical researchers. One way of doing that is by using Addgene’s Blue Flame award to identify...

  13. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ... enter the nucleus through nuclear pores and thus do not require breakdown of the nuclear envelope during...division to enter the nucleus as gamma-retroviruses do. Fun fact: Since lentivirus and gamma-retrovirus...

  14. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    .... That’s also a great way to get to know people doing interesting and relevant work in your field! However...single well in a 96-well (or 384-well) plate! How much do repositories help?   Designing precise calculations...

  15. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...questions: What is the goal, who is my audience, and what do I want to be different for them when I’m done? What... Excellent! We asked our workshop participants to do the same thing, and even though only about 10% of...

  16. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...your email for confirmation of submission. If you do not receive such a notice within 24 hrs, send a note...while you are climbing the academic ladder. If you do not get accepted to your top choice this year but...

  17. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    .... Unlike cytoskeletal polymers, however, R bodies do not exert force by dynamically rearranging into different...bodies can be found at Addgene here. What can R bodies do for you? R bodies are robust linear actuators that...

  20. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ... the bacterial elements previously described. Why do scientists use yeast vectors? Yeast are eukaryotes... Plasmids for use in S. pombe, on the other hand, do not require a well defined ORI. Instead, the size...
Showing: 281 - 300 of 620 results