Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 593 results for: ELP

Showing: 281 - 300 of 593 results

  2. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...we wish to conduct research that fails less and helps more people, we need to take sex into account. Gendered...Physiology over the past decade. For more practical help, there are numerous methodological articles to consider—we...

  3. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...can use primer design software such as Primer3 to help you design your custom primers. Remember, when designing...forward and reverse primers for each sample with the help of DNA-editing software which integrates our database...

  5. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...accomplish all of that? What follows are some tips to help you build a great poster.  Tips for designing your...one for the body of those sections. This strategy helps your sections stand out, and makes the entire poster...

  6. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...the practical application of the plasmid and will help a scientist outside your field understand if this...up-to-date version! We hope the deposit spreadsheet will help expedite your data entry and make it easier than...

  8. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...it be fully sequenced by Addgene’s QC team. Email help@addgene.org to get started! Addgene’s QC process...the raw NGS reads to a reference sequence can also help QC scientists determine if enough reads were acquired...

  9. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...details. You can find many software programs to help you design pegRNAs. Here on the Addgene blog, we...a guest blog by the developer of PRIDICT, which helps users select pegRNA designs that are most likely...

  10. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...Addgene!) We’re excited to help the Doench lab share this new tool and hope it helps you create great plasmids...

  11. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...family Parvoviridae and requires co-infection of helper viruses like adenovirus for productive infection...and the selection of specific promoters may also help AAV vectors evade host immunity. While basic research...

  12. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...America. Diagnostics will play an important role in helping to monitor and slow this spread until vaccine programs...toehold switches and amplification primers. This helped make sure that we had a number of successful sensors...

  13. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...Sticky-end mediated gene transfer would be particularly helpful for targeting non-dividing cells, which are difficult...Exploring the efficiency of these two processes will help us better understand where Cpf1 fits into the genome...

  14. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...learn all about ddPCR for AAV titration and find helpful tips and tricks in our blog post “Droplet Digital... This is an extremely useful PCR method that can help save time and effort in the laboratory.  There are...

  15. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...current methods, understanding the differences can help you make an informed decision. Mammalian cells Mammalian...on these differences in viral production and will help you decide which is right for you. Table 1: Mammalian...

  16. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...they have collected a variety of data that might help formulate some hypotheses. They are excited to see...troubleshooting tips and works with other labs to help them adopt the tools his group develops. And though...

  17. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...is your desired library coverage, since this will help decide what transformation efficiency you need to...plates). In general, amplification on plates can help to ensure that diversity of the library is maintained...

  18. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ... CRISPR experiments, the following framework can help get you started: Determine what type of outcome...domains to control gene expression. Base editors can help you make precise point mutations near the gRNA target...

  19. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...fragments You might be wondering, “How is milk going to help me extract DNA from agarose gel?!”   The DNA gel... it solubilizes both DNA and agarose, and 2) it helps DNA stick to the glass. After binding DNA, the glass...

  20. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...AAV-RPE65 treatment, famously visited Congress to help increase awareness about the potential of gene therapy...and colleagues at MEEI co-founded a non-profit to help push forward candidate therapies for these diseases...
Showing: 281 - 300 of 593 results