We narrowed to 553 results for: ESC
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TypeBlog PostPublishedOct. 7, 2016, 4 p.m....Jessica Sacher from the University of Alberta and describes a technique you can use to find phage proteins...molecular biology tool, P1 phage. 2) The post describes an ingenious but simple technique to screen for...
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IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging
TypeBlog PostPublishedJune 8, 2023, 1:15 p.m.... reagents from 20 contributors, including 61 fluorescent probes, representing imaging from 47 different...the Addgene blog Antibodies 101: Multiplex Immunofluorescence Negative Can Be Positive: Open AAV Data with... -
Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry
TypeBlog PostPublishedJune 23, 2020, 1:15 p.m.... Michael Davidson Fluorescent Protein collection, a ‘One-Stop Shop’ for Fluorescent Proteins, and plasmids... -
What's New in CRISPR - September 2019
TypeBlog PostPublishedSept. 10, 2019, 12:56 p.m....Cas9 and a donor vector to create knock-ins of fluorescent proteins, fusion proteins, or transcriptional...interest and on either side of the cargo (ex: fluorescent protein gene) on the donor vector. Therefore,... -
Working 9-5: an Addgene Co-op
TypeBlog PostPublishedJune 2, 2022, 2:15 p.m....process When I first saw the comprehensive job description for this co-op, I was immediately interested ...had stood out to me because it gave me a full description of the tasks I would be responsible for, in comparison... -
Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening
TypeBlog PostPublishedFeb. 20, 2014, 4:57 p.m....Two new human lentiviral CRISPR library systems described in companion papers in Science in December were...Additionally, a concurrent Nature Biotechnology article describes the development of a mouse lentiviral CRISPR library... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog PostPublishedMay 21, 2020, 1:15 p.m.... optogenetic tool (CsChrimson) attached to a fluorescent indicator (tdTomato), as the effector. They also...expression in flies Resources on Addgene.org Find fluorescent protein plasmids at Addgene Read our Molecular... -
Addgene at the ASM Microbe 2016 Conference
TypeBlog PostPublishedJuly 7, 2016, 2:30 p.m....fascinating and fun look at her outreach work with fluorescent microbes. Check out her animation about the Hawaiian...repository (like these new Rainbow Bacterial Fluorescence Labeling Plasmids from the Barbier lab). Bright... -
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
TypeBlog PostPublishedJune 27, 2018, 1:16 p.m....to bind fluorescent proteins derived from A. victoria the functionalized nanobodies described here can... -
Plasmids 101: Origin of Replication
TypeBlog PostPublishedNov. 11, 2020, 2:23 p.m....p15A B Relaxed pSC101 ~5 pSC101 C Stringent pBluescript ~300-500 ColE1 (derivative) and F1** A Relaxed...multiple-unnatural amino acid mutagenesis in Escherichia coli. Biochemistry. 2013 Mar 12;52(10):1828-37... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog PostPublishedAug. 8, 2019, 1:19 p.m....of my review - "The manuscript by ZZZ et al., describes ... The authors share a wealth of data on ... ...Additionally, you can ask that data and resources described in the manuscript are accessible to the community... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog PostPublishedMay 9, 2019, 1:15 p.m....Direct reprogramming describes the process where differentiated cells are turned into a cell type of ...reprogramming factors for generating iPSCs Learn how fluorescent proteins help researchers study single cell dynamics... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog PostPublishedJune 17, 2021, 1:17 p.m....will not produce the fluorescent protein while areas that are exposed will fluoresce. With these images,... -
Addgene Begins Distribution of Recombinant Antibodies
TypeBlog PostPublishedMarch 28, 2022, 4:30 p.m....Antibody Guide, which include information about immunofluorescence, conjugates, isotypes, and more. How You ...Basics of Western Blotting Introduction to Immunofluorescence Addgene’s Antibody Guide The project is ... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog PostPublishedDec. 21, 2017, 2:06 p.m.... a feature inaccurately. The best example is fluorescent proteins, which often come in closely related...misidentification, we augment the database with new fluorescent protein variants. The future of DNA feature ... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog PostPublishedSept. 15, 2015, 2:30 p.m....as their kidneys fail. The condition was first described more than 60 years ago, but there is still no ...changes in temperature. “The disease was first described in 1971,” McSweeney said. “But, it is still sometimes... -
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog PostPublishedMarch 4, 2014, 4:49 p.m....published in Nature Methods, Boyden and his team describe the discovery of a light-sensitive opsin from ...then, there has been a steady stream of papers describing new photosynthetic or photosensory molecules ... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog PostPublishedJuly 17, 2018, 12:23 p.m....and more details on how to play the game on the FRESCI blog. As I wrote in the blog post, BUILD YOUR PLASMID...Barcelona. He is also founder and consultant at FRESCI particularly interested in advanced in vitro models... -
New and Upcoming Viral Vectors - June 2019
TypeBlog PostPublishedJune 18, 2019, 1:15 p.m....state, which was achieved by lowering the basal fluorescence of the sensor. This “contrast” variant provides... studies in non-human primates, where fusion fluorescent proteins can affect DREADD trafficking and localization... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog PostPublishedApril 9, 2020, 1:15 p.m....s time, we’ve published an article on BioRxiv describing a comprehensive SARS-CoV-2 virus-human interaction...other labs at UCSF (the QCRG network coming to the rescue -- again!). Also, we’ve got to mention that our...