Skip to main content
Addgene
Showing: 281 - 300 of 543 results

  1. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...Cas9 and a donor vector to create knock-ins of fluorescent proteins, fusion proteins, or transcriptional...interest and on either side of the cargo (ex: fluorescent protein gene) on the donor vector. Therefore,...

  2. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ...Two new human lentiviral CRISPR library systems described in companion papers in Science in December were...Additionally, a concurrent Nature Biotechnology article describes the development of a mouse lentiviral CRISPR library...

  3. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ... optogenetic tool (CsChrimson) attached to a fluorescent indicator (tdTomato), as the effector. They also...expression in flies Resources on Addgene.org Find fluorescent protein plasmids at Addgene Read our Molecular...

  4. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    Published
    July 7, 2016, 2:30 p.m.
    ...fascinating and fun look at her outreach work with fluorescent microbes. Check out her animation about the Hawaiian...repository (like these new Rainbow Bacterial Fluorescence Labeling Plasmids from the Barbier lab). Bright...

  5. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ... a feature inaccurately. The best example is fluorescent proteins, which often come in closely related...misidentification, we augment the database with new fluorescent protein variants. The future of DNA feature ...

  6. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...Direct reprogramming describes the process where differentiated cells are turned into a cell type of ...reprogramming factors for generating iPSCs Learn how fluorescent proteins help researchers study single cell dynamics...

  8. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...Antibody Guide, which include information about immunofluorescence, conjugates, isotypes, and more. How You ...Basics of Western Blotting Introduction to Immunofluorescence Addgene’s Antibody Guide The project is ...

  10. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...p15A B Relaxed pSC101 ~5 pSC101 C Stringent pBluescript ~300-500 ColE1 (derivative) and F1** A Relaxed...multiple-unnatural amino acid mutagenesis in Escherichia coli. Biochemistry. 2013 Mar 12;52(10):1828-37...

  12. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...as their kidneys fail. The condition was first described more than 60 years ago, but there is still no ...changes in temperature.  “The disease was first described in 1971,” McSweeney said. “But, it is still sometimes...

  14. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...s time, we’ve published an article on BioRxiv describing a comprehensive SARS-CoV-2 virus-human interaction...other labs at UCSF (the QCRG network coming to the rescue -- again!). Also, we’ve got to mention that our...

  15. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...producer cells and purification protocols often describe a process that begins with cell lysis to release...Central PMCID: PMC4104587. György, Bence, et al. "Rescue of hearing by gene delivery to inner-ear hair cells...

  16. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...and more details on how to play the game on the FRESCI blog. As I wrote in the blog post, BUILD YOUR PLASMID...Barcelona. He is also founder and consultant at FRESCI particularly interested in advanced in vitro models...

  17. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...state, which was achieved by lowering the basal fluorescence of the sensor. This “contrast” variant provides... studies in non-human primates, where fusion fluorescent proteins can affect DREADD trafficking and localization...

  18. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ... of the major CRISPR lingo and will be able to describe the functions of the various CRISPR/Cas9 components...Bind to a guide RNA The guide RNA, which will be described in more detail below, enables Cas9 to cut a specific...

  19. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...mammalian plasmid components, it may be useful to describe the means of introducing genetic material (such...the plasmid in the same way. Selection methods, described below, are typically employed when creating stable...
Showing: 281 - 300 of 543 results