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We narrowed to 867 results for: FER

Showing: 281 - 300 of 867 results

  1. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    Published
    Sept. 23, 2014, 3:05 p.m.
    ...Labguru, Quartzy offers software designed with scientists workflow in mind and can offer a one-stop solution...create lists of your favorite protocols for future reference. The exchange currently boasts over 2,500 protocols... is a subscription-based software, Labguru does offer free trials and pricing options for academic labs... limited to within your lab group, Labguru does offer access to protcols from external vendors, such as...orders. Quartzy caters to life science labs and offers the service free of charge to scientists by allowing...101 articles on various plasmid features, and reference tables (i.e. antibiotic concentrations, commonly...

  2. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    Published
    Aug. 27, 2020, 1:15 p.m.
    ...Forster Resonance Energy Transfer (FRET). Through the non-radiative transfer for energy between neighboring...requires careful selection of FP fragments and, preferably, some information about the structures of the...level, stability, and subcellular localization may differ from those of your BiFC construct, making it difficult...., do two proteins interact or not? — FRET is preferable when the temporal dynamics of PPIs are the focus...about science whenever he's not off the grid.   References Bischof J, Duffraisse M, Furger E, Ajuria L, ...plasmids and resources Visit our Molecular Biology Reference ...

  3. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...decorative" title="Check icon" %}   References and Resources References Hwang, J. M., Piccinini, T. E., Lammel...belongs to the penicillin family of antibiotics. It interferes with cell wall synthesis as its mode of action...beta-lactamase, just like amp is. Thus, the AmpR gene confers resistance to both carb and amp, due to their common...amp), and for some applications, the benefit it offers is marginal. Fortunately, amp can easily be replaced...phosphorylation of kan, which inactivates it. The NPTII gene confers resistance to several aminoglycosides, including...minutes). Pros: Widely available, cost-effective, confers mammalian resistance to G418. Con: Slow transformation...

  4. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...sample(s) in the same buffer if possible, or very similar buffers if not, since buffer components can affect...non-linear and you should use a different method of analysis. Different fields and assays have varying ...the assay is run, ideally with freshly prepared buffer. This is because standard curves vary depending...range covers a wider span of concentrations but suffers from decreased specificity, especially as it approaches...

  5. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...antibodies directly into your preferred buffer. If that’s the case, a buffer exchange can be performed using...downstream step, you’ll want to ensure that your buffer components are compatible with your conjugate and...you’ve collected your antibody in the appropriate buffer, it’s time to quantify it. There are several options...our Antibodies 101 blog series.  Resources and references More resources on the Addgene blog Introduction...Purification Protocol Addgene's Antibody Guide References Trimmer JS. Recombinant Antibodies in Basic Neuroscience...

  6. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...created with the same backbone but that contain different inserts. When you design primers to anneal outside...primer pair to screen for the presence of many different inserts. The downside: this type of primer does... The type of primers you use depends on your preferences. Either way, make sure to test your colony PCR...a standard PCR thermocycling program. One key difference is the plasmid DNA must be released from the ...and without an insert. These controls are quick references you can use when you run your PCR products out... an appropriate molecular weight standard for reference and to add a loading dye with glycerol to your...products have any mutations.  There are lots of different cloning strategies, but regardless of which is...

  7. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...PAM sequence requirements. Cpf1’s preferred PAM is 5’-TTN, differing from that of Cas9 (3’-NGG) in both...endonuclease domain, indicating that Cpf1 functions differently. Since Cpf1 loci are widely distributed across...could be adapted for genome editing. The use of a different nuclease could potentially overcome some of Cas9...pattern opens up the possibility of directional gene transfer, analogous to traditional restriction enzyme cloning... cloning. Sticky-end mediated gene transfer would be particularly helpful for targeting non-dividing cells...shown the diversity of CRISPR-based systems. References 1. Zetsche, Bernd, et al. "Cpf1 is a single RNA-guided...

  8. Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service

    Type
    Blog Post
    Published
    July 28, 2020, 1:15 p.m.
    ...preparative experiments.  We’re excited to begin offering this service to further contribute to our mission...and discovery.” Here are the details. Plasmids offered as cloning grade DNA As we begin testing out this...that this service will be helpful to the many different scientific communities using plasmid tools. Details... We will ship you  2 µg of DNA in 20 µl of Tris buffer suitable for molecular biology experiments such... our Plasmids 101 blog series Learn about the different grades of DNA  Read about the various ways to ...other beta projects Read our Molecular Biology Reference ...

  9. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...Cary Valley, and Holly McQueary References and Resources References Niedringhaus, T. P., Milanova, D....identify the bases (and, if necessary, separate different samples), the resulting data, or reads, are essentially...plasmid sequence. However, if there are major differences from the expected sequence, the QC team can manually...theoretical sequences submitted to Addgene have minor differences due to in silico variations and lack of verification...script for manually inputting known innocuous differences. This script compares the sequence for a 100%...Additional Resources on addgene.org Molecular Biology Reference Sequence Analyzer Protocol: Sequence Analysis ...

  10. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...proteins also contribute to this regulation in different ways: methylating newly synthesized DNA, blocking...numbers can allow RepA to link iterons from two different plasmids, forming a pair of “handcuffs” that blocks...RepA proteins form complexes between iterons on different plasmids in a process called "handcuffing," which...Plasmids under stringent control of replication may differ in the specialized proteins or sequences necessary...was updated by Emily P. Bentley in June 2024.  References and resources Further Reading Quiñones-Valles...Replication. IntechOpen. https://doi.org/10.5772/19313 References Asano, K., & Mizobuchi, K. (1998). Copy number...molecular biology help with our Molecular Biology Reference Pages   ...

  11. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...best with diverse teams, to leverage the preferences of different personality types, and to devise strategies...biotech clients. In this realm, there are a few different kinds of challenges that consultants get called...consulting experiences, educational training, and conferences, and synthesizing this knowledge into a coherent...excessively demanding. For instance, a client may offer a lot of pointed feedback or abruptly change needs...consulting firms coach and counsel employees on the different personality and work styles (e.g., Myers-Briggs...prepare you for a successful career in several different fields. While some people continue to enjoy the...

  12. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...example, CRISPR activation has been used to differentiate induced pluripotent stem cells (Chavez et al...neuronal cells by activating genes that cause differentiation and to reverse HIV viral latency (Bialek et...directed towards that gene as there can be a large difference in guide potency. Thus, in cases where users ...cannot be used to study in cis regulation and also suffer from an inability to easily deliver the appropriate...Chavez et al., 2016). To learn more about the different types of Cas9 activators, check out our blog post...DNA sequence or regulating gene expression.     References Behan FM, Iorio F, Picco G, Gonçalves E, Beaver...s41586-019-1103-9 Bialek JK, Dunay GA, Voges M, Schäfer C, Spohn M, Stucka R, Hauber J, Lange UC (2016)...

  13. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...today. It is now known that nearly 500 cell lines suffer from contamination with HeLa being the most frequent...Down-Regulation of HIF-1α by NDRG2 Expression Attenuates Proliferation and Invasion in Renal Cancer Cells, Marker-independent...the cell lines deposited with them and used by different researchers are free of contamination and match...from this bank. I am often asked, “What is the difference between materials available from commercial repositories...the same cell line from a colleague?”. The main difference is the QC and validation carried out by the commercial...which can be stored in liquid nitrogen long term, preferably some of these vials will be stored in a separate... drug testing. You can find Nick on LinkedIn. References 1. Skloot, Rebecca, and Bahni Turpin. The immortal...

  14. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    ...fragments that are very different.” Because the fragments produced will have different overhangs, they can...enzymes. Now, a new toolkit - aptly named GreenGate – offers all of those same advantages to researchers working...systems. “The cloning is all done in vitro; it’s no different in mouse, human or plants,” explained Jan Lohmann...Lohmann of Heidelberg University. “What is different is the plasmids you use to bring this into your target...available, making it easier for us to do our work.” References 1. Athanasios Lampropoulos et al. "GreenGate ...

  15. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...similarities with humans. However, rats have some key differences in physiology, intelligence, and human correspondence... generate transgenic axolotls for an array of different studies. Elly Tanaka’s lab has constructed several...number of offspring (produces many seeds), self fertilizing, a small yet highly characterized genome, and...include understanding of allelic variation between different plant species and metabolic properties affecting... collections to help start your experiments! References: Blount, Z. D. (2015). The unexhausted potential...Zhang, C., & Xing, X.-H. (2018). Pooled CRISPR interference screening enables genome-scale functional genomics...Plasmids Collection Page Read our molecular biology reference Find plasmids for your research ...

  16. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...this results in the formation of a DNA structure referred to as the displacement loop (D loop). The recombination...when selecting a type of donor. ssDNA templates (referred to as ssODNs (oligodeoxynucleotides)) are commonly... difficult, thus dsDNA plasmids are generally preferred for large insertions such as fluorescent proteins... blog post on Easi-CRISPR for more details. References and resources Want to know more? Check out the...Genome Editing General Homologous Recombination References: Repair of Strand Breaks by Homologous Recombination...their potential to induce chromosomal aberrations. Pfeiffer P, et al. Mutagenesis (2000). PubMed. Multiple...Cas-mediated Transgenesis (2018). Pubmed. CRISPR References: Genome engineering using the CRISPR-Cas9 system...

  17. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ... science) they come from many different places supporting many different positions. It would be detrimental... and everyone is welcome. Why? There are many different things scientists and non-scientists alike are...not necessarily marching for every cause. The references provided also only provide a snapshot into the...microbial interactions, and science education.       References March for Science Website Locke, John, and Peter...York Times: With Trump in Charge, Climate Change References Purged from Website The Washington Post: Trump...The scientific community: Diversity makes the difference  Association for Psychological Science: Diversity...

  18. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...research coming out of the lab.  References and resources References Hao, Y., Yan, J., Fraser, C., Jiang...integrins at non-ligand-binding sites and do not interfere with integrin function. Overall, the synthetic...then used these antibodies to show the binding preferences of the αV subunit and its β partners. Of interest...contrast, LVDP-binding integrin α4β1 required and conferred less force, leading cells to spread into symmetrical...antibodies and affinity reagents to study the difference in cell morphology changes induced by integrins...discovered by yeast display reveal αV subunit pairing preferences with β subunits (p. 2024.01.26.577394). bioRxiv...

  19. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...purified protein at different acid concentrations. Overviews of the pKa values of different fluorescent protein... GFP variants. Here I use the name ‘GFP’, which refers to mEGFP. This variant was made by introducing ...reduces its size. The substantial size of GFP may interfere with the activity or the localization of the fusion... Another solution to avoid autofluorescence is offered by bioluminescent proteins. These probes generate...C-terminus of another protein. This may, however, interfere with the function of the protein. This is clearly...can follow him on twitter: @joachimgoedhart.   References 1. Zacharias, David A., et al. "Partitioning ...

  20. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    .... The plasmids within it contain a variety of different promoters followed by leader sequences (encoding... at the BIOFAB showed that expression of many different genes was predictable based solely on the promoters...or protease sites to remove the epitope tags. Different plasmids in the kit can be used to purify proteins...prokaryotic expression in recent years, with many different systems now available. However, it can be difficult...ORF clones to allow testing and comparison of different cell-free expression systems; the Cell-free expression...bind specific RNA bases. By swapping out these different modular repeat segments, you can create a PUF ...proteins with a wide variety of functions via different fusions. Some applications of PUF protein fusions...
Showing: 281 - 300 of 867 results