We narrowed to 303 results for: GAI

1. Technologies Enabled by NanoLuc® Luciferase

   Type

   Blog Post

   Published

   Feb. 8, 2018, 12:17 p.m.

   ...and Red (ReNL; plasmid #85203) NLuc fusions. The Nagai lab has additionally deposited more than 25 fusions...

2. Technique: Probe Phage Genomes for Host Binding Proteins

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   Blog Post

   Published

   Sept. 29, 2016, 2:30 p.m.

   ...of whole phages as therapeutics, which is also gaining a lot of new traction lately (1, 2), depends on...

3. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

   Type

   Blog Post

   Published

   Nov. 2, 2021, 1:15 p.m.

   ...modifications, and other factors that AAV utilizes to gain entry into the cell (Dudek, AM. 2018).  If the serotype...

4. Plasmids 101: Using Transposons in the Lab

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   Blog Post

   Published

   July 6, 2021, 1:28 p.m.

   ...mutagenesis screens that detect loss-of-function or gain-of-function mutations. In these screens, the transposons...

5. When Fidelity Matters: A frank discussion about ligase fidelity

   Type

   Blog Post

   Published

   March 6, 2018, 1:49 p.m.

   ...DNA ligase, are naturally able to discriminate against ligating substrates containing base pair mismatches...

6. Quick Guide to All Things Lentivirus

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   Blog Post

   Published

   March 21, 2017, 2:30 p.m.

   ...multiplicity of infection (MOI=ratio of virus to cells). Again, my tip here is to test several protocols as the...

7. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

   Type

   Blog Post

   Published

   Dec. 15, 2016, 3:57 p.m.

   ...temperature allows the repressor to re-nature and again repress expression of exo, beta and gam.  A major...

8. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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   Blog Post

   Published

   June 28, 2018, 3:01 p.m.

   ...complexes - from the cell-lysate using antibodies against the target protein. The captured protein complexes...

9. Typing CRISPR Systems

   Type

   Blog Post

   Published

   March 18, 2025, 1:15 p.m.

   ...principles of this classification system will help you gain a deeper appreciation and understanding of CRISPR...

10. Antibodies 101: Designing Your First Flow Panel

    Type

    Blog Post

    Published

    May 28, 2024, 1:45 p.m.

    ...briefly go through each section of this article again. In the beginning, we talked about the relationship...

11. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type

    Blog Post

    Published

    Aug. 15, 2017, 1:24 p.m.

    ...lymphocytes from the bone marrow to the thymus where they gain their specificity or from the thymus to the lymph...

12. Special Delivery: Fluorophore Targeting for FRET Studies

    Type

    Blog Post

    Published

    July 19, 2016, 2:30 p.m.

    ...in any NIH grant application! Using FRET, you may gain a new structural understanding of your protein that...

13. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type

    Blog Post

    Published

    Sept. 10, 2015, 2:30 p.m.

    ...for sufficient opsin to reach the axon terminal. Again, this must be empirically determined in your own...

14. A Needle in a Base-Stack: Cas9 Structural Biology

    Type

    Blog Post

    Published

    June 4, 2024, 1:15 p.m.

    ...precise location. The end of the heteroduplex rests against the RuvC nuclease domain, which stabilizes the ...

15. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type

    Blog Post

    Published

    Jan. 13, 2025, 9:49 p.m.

    ...pick one or the other. Which prime editor is which again? We’re glad you asked. Hopefully this table helps...

16. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type

    Blog Post

    Published

    Nov. 3, 2015, 3:30 p.m.

    ...succeed. With respect to de novo sgRNA design, I again separate it into two categories. On the one hand...

17. Deep Dive: qPCR

    Type

    Blog Post

    Published

    Sept. 29, 2022, 1:15 p.m.

    ...run and the difference in CT values is plotted against the log input concentration for each dilution. ...

18. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type

    Blog Post

    Published

    Sept. 3, 2020, 12:15 p.m.

    ...rendering the Cas9 non-functional when split but regaining functionality when the halves are reassociated...

19. Delivery Methods for Generating iPSCs

    Type

    Blog Post

    Published

    April 17, 2018, 1:37 p.m.

    ...used to protect the RNAs from cellular defenses against ssRNA: phosphatase treatment, substituting cytidine...

20. Using AAV for Neuronal Tracing

    Type

    Blog Post

    Published

    Aug. 9, 2018, 1:04 p.m.

    ...is taken up on one side of the cell and released again at another side of the cell after intracellular ...