We narrowed to 307 results for: GAI

1. Technologies Enabled by NanoLuc® Luciferase

   Type

   Blog Post

   Published

   Feb. 8, 2018, 12:17 p.m.

   ...and Red (ReNL; plasmid #85203) NLuc fusions. The Nagai lab has additionally deposited more than 25 fusions...

2. How to Design Your gRNA for CRISPR Genome Editing

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   Blog Post

   Published

   Sept. 24, 2020, 1:15 p.m.

   ...signal in these assays (Dempster et al., 2019). Again, the experimental strategy is clear: for any gene...

3. Hot Plasmids: Spring 2025

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   Blog Post

   Published

   June 3, 2025, 1:15 p.m.

   ... set of highly-specific recombinant antibodies against three of the six human glypican proteins (GPC1,...

4. Technique: Probe Phage Genomes for Host Binding Proteins

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   Blog Post

   Published

   Sept. 29, 2016, 2:30 p.m.

   ...of whole phages as therapeutics, which is also gaining a lot of new traction lately (1, 2), depends on...

5. Antibodies 101: The Basics of Western Blotting

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   Blog Post

   Published

   March 8, 2021, 2:41 p.m.

   ...blocked, it is stained with a primary antibody against your protein of interest. But which antibody should...

6. When Fidelity Matters: A frank discussion about ligase fidelity

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   Blog Post

   Published

   March 6, 2018, 1:49 p.m.

   ...DNA ligase, are naturally able to discriminate against ligating substrates containing base pair mismatches...

7. Quick Guide to All Things Lentivirus

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   Blog Post

   Published

   March 21, 2017, 2:30 p.m.

   ...multiplicity of infection (MOI=ratio of virus to cells). Again, my tip here is to test several protocols as the...

8. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

   Type

   Blog Post

   Published

   Dec. 15, 2016, 3:57 p.m.

   ...temperature allows the repressor to re-nature and again repress expression of exo, beta and gam.  A major...

9. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

   Type

   Blog Post

   Published

   Nov. 2, 2021, 1:15 p.m.

   ...modifications, and other factors that AAV utilizes to gain entry into the cell (Dudek, AM. 2018).  If the serotype...

10. Plasmids 101: Using Transposons in the Lab

    Type

    Blog Post

    Published

    July 6, 2021, 1:28 p.m.

    ...mutagenesis screens that detect loss-of-function or gain-of-function mutations. In these screens, the transposons...

11. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type

    Blog Post

    Published

    June 28, 2018, 3:01 p.m.

    ...complexes - from the cell-lysate using antibodies against the target protein. The captured protein complexes...

12. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type

    Blog Post

    Published

    Aug. 15, 2017, 1:24 p.m.

    ...lymphocytes from the bone marrow to the thymus where they gain their specificity or from the thymus to the lymph...

13. Typing CRISPR Systems

    Type

    Blog Post

    Published

    March 18, 2025, 1:15 p.m.

    ...principles of this classification system will help you gain a deeper appreciation and understanding of CRISPR...

14. Special Delivery: Fluorophore Targeting for FRET Studies

    Type

    Blog Post

    Published

    July 19, 2016, 2:30 p.m.

    ...in any NIH grant application! Using FRET, you may gain a new structural understanding of your protein that...

15. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type

    Blog Post

    Published

    Sept. 10, 2015, 2:30 p.m.

    ...for sufficient opsin to reach the axon terminal. Again, this must be empirically determined in your own...

16. Antibodies 101: Designing Your First Flow Panel

    Type

    Blog Post

    Published

    May 28, 2024, 1:45 p.m.

    ...briefly go through each section of this article again. In the beginning, we talked about the relationship...

17. A Needle in a Base-Stack: Cas9 Structural Biology

    Type

    Blog Post

    Published

    June 4, 2024, 1:15 p.m.

    ...precise location. The end of the heteroduplex rests against the RuvC nuclease domain, which stabilizes the ...

18. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type

    Blog Post

    Published

    Jan. 13, 2025, 9:49 p.m.

    ...pick one or the other. Which prime editor is which again? We’re glad you asked. Hopefully this table helps...

19. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type

    Blog Post

    Published

    Nov. 3, 2015, 3:30 p.m.

    ...succeed. With respect to de novo sgRNA design, I again separate it into two categories. On the one hand...

20. Delivery Methods for Generating iPSCs

    Type

    Blog Post

    Published

    April 17, 2018, 1:37 p.m.

    ...used to protect the RNAs from cellular defenses against ssRNA: phosphatase treatment, substituting cytidine...