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We narrowed to 377 results for: HAL

Showing: 281 - 300 of 377 results

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Plasmids for Endogenous Gene Tagging in Human Cells

Type

Blog Post

Published

April 6, 2017, 1:02 p.m.

...Institute website at alleninstitute.org. A classic challenge in cell biology is making sure that what we observe...
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

Type

Blog Post

Published

March 8, 2017, 3:15 p.m.

... (budding yeast), S. pombe (fission yeast), A. thaliana (mustard weed), O. cuniculus (rabbit), synthetic...
Adenoviral Vector Production and Troubleshooting

Type

Blog Post

Published

June 14, 2018, 11:44 a.m.

...not integrate into the host’s genome. The main challenge with using AdV is that it triggers a strong immune...
Pathways Over Time Plasmids Engage Students in Functional Genomics Research

Type

Blog Post

Published

May 22, 2018, 1:38 p.m.

...students (1, 2), but educators face significant challenges in designing suitable projects. In the O'Connor...
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

Type

Blog Post

Published

May 15, 2018, 12:43 p.m.

...codon-optimized cryptochrome 2 (CRY2) from Arabidopsis thaliana. When exposed to blue light, CRY2 undergoes a ...
Split-BioID: An Improved Method for Studying Protein-Protein Interactions

Type

Blog Post

Published

May 1, 2018, 1:57 p.m.

... make it difficult to detect proteins with short half-lives. Interested in using Split-BioID with your...
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

Type

Blog Post

Published

Sept. 26, 2019, 12:50 p.m.

...pronucleus of the zygote which is both technically challenging and can negatively impact zygote viability. Nanoblades...
Mouse Modeling, Part 1: Genetically Engineered Mice

Type

Blog Post

Published

July 11, 2019, 1:26 p.m.

...the injection of a targeting vector alone, some challenges still exist. Nucleases may cause off-target effects...
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

Type

Blog Post

Published

Nov. 14, 2024, 2:15 p.m.

...in Plants with the CRISPR-Cas Technology is a Challenge yet Worth Accepting. Front Plant Sci. 2015;6. ...
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

Type

Blog Post

Published

Oct. 6, 2020, 1:15 p.m.

...Ter-Ovanesyan D, Haque SJ, Cecchi RJ, Kowal EJK, Buchthal J, Housden BE, Perrimon N, Collins JJ, Church ...
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

Type

Blog Post

Published

July 23, 2020, 1:20 p.m.

...that have the gene deletion could replicate when challenged with the S. islandicus CRISPR-Cas system. 5. ...
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

Type

Blog Post

Published

April 6, 2021, 12:30 p.m.

...DNA Extracts. Pooled libraries are a bit more challenging to QC by NGS as they are often quite different...
Hot Plasmids and Viral Preps - January 2021

Type

Blog Post

Published

Jan. 5, 2021, 2:15 p.m.

... is only ~70kDa, with a molecular weight roughly half of Cas9 or Cas12a. This is in part due to the fact...
Adapting Toehold Switches to Detect Zika Virus

Type

Blog Post

Published

Aug. 30, 2016, 2:30 p.m.

...symptoms, including the development of fetal microcephaly and Guillain-Barré syndrome. In response, the...
The Materials Science of Optogenetics Experiments

Type

Blog Post

Published

Sept. 17, 2015, 2:30 p.m.

...validation experiments we discussed last week are only half of the battle when using optogentics to answer a...
Experimenting in an Open Source lab: from CRISPR to Cats

Type

Blog Post

Published

July 23, 2015, 2:30 p.m.

... case. Funding Makerspaces One of the main challenges when starting a new project at a makerspace is...
Hot Plasmids: Winter 2025

Type

Blog Post

Published

March 11, 2025, 1:15 p.m.

... developed in human cells, has been uniquely challenging to apply in dicotyledonous plants, or dicots....
The AAVantages of AAV in CRISPR Screens

Type

Blog Post

Published

Feb. 4, 2025, 2:15 p.m.

.... V., Teyssier, N., Pan, A., Danner-Bocks, S., Sanghal, T., Yadanar, L., Tian, R., Ma, K., Palop, J. J...
Truncated gRNAs for Regulating Gene Expression

Type

Blog Post

Published

Jan. 10, 2017, 3:37 p.m.

...different perturbations. Consider, for instance, the challenge of attempting to simultaneously and selectively...
Plasmids 101: 5 factors to help you choose the right cloning method

Type

Blog Post

Published

Aug. 21, 2018, 12:31 p.m.

...technologies currently available, you now face the challenge of trying to figure out what system might work...

Showing: 281 - 300 of 377 results

Results per page:

Plasmids for Endogenous Gene Tagging in Human Cells

Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

Adenoviral Vector Production and Troubleshooting

Pathways Over Time Plasmids Engage Students in Functional Genomics Research

Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

Split-BioID: An Improved Method for Studying Protein-Protein Interactions

Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

Mouse Modeling, Part 1: Genetically Engineered Mice

CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

Hot Plasmids and Viral Preps - January 2021

Adapting Toehold Switches to Detect Zika Virus

The Materials Science of Optogenetics Experiments

Experimenting in an Open Source lab: from CRISPR to Cats

Hot Plasmids: Winter 2025

The AAVantages of AAV in CRISPR Screens

Truncated gRNAs for Regulating Gene Expression

Plasmids 101: 5 factors to help you choose the right cloning method

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