We narrowed to 378 results for: Hal

1. Plasmids 101: Cre-lox

   Type

   Blog Post

   Published

   Jan. 13, 2015, 3:47 p.m.

   ...in a particular organ, or bypassing embryonic lethality. To facilitate the use of Cre-lox technology,...

2. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

   Type

   Blog Post

   Published

   Nov. 14, 2024, 2:15 p.m.

   ...in Plants with the CRISPR-Cas Technology is a Challenge yet Worth Accepting. Front Plant Sci. 2015;6. ...

3. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

   Type

   Blog Post

   Published

   Sept. 26, 2019, 12:50 p.m.

   ...pronucleus of the zygote which is both technically challenging and can negatively impact zygote viability. Nanoblades...

4. Mouse Modeling, Part 1: Genetically Engineered Mice

   Type

   Blog Post

   Published

   July 11, 2019, 1:26 p.m.

   ...the injection of a targeting vector alone, some challenges still exist. Nucleases may cause off-target effects...

5. Adenoviral Vector Production and Troubleshooting

   Type

   Blog Post

   Published

   June 14, 2018, 11:44 a.m.

   ...not integrate into the host’s genome. The main challenge with using AdV is that it triggers a strong immune...

6. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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   Blog Post

   Published

   May 22, 2018, 1:38 p.m.

   ...students (1, 2), but educators face significant challenges in designing suitable projects. In the O'Connor...

7. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

   Type

   Blog Post

   Published

   May 15, 2018, 12:43 p.m.

   ...codon-optimized  cryptochrome 2 (CRY2) from Arabidopsis thaliana. When exposed to blue light, CRY2 undergoes a ...

8. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

   Type

   Blog Post

   Published

   May 1, 2018, 1:57 p.m.

   ... make it difficult to detect proteins with short half-lives. Interested in using Split-BioID with your...

9. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

   Type

   Blog Post

   Published

   Oct. 6, 2020, 1:15 p.m.

   ...Ter-Ovanesyan D, Haque SJ, Cecchi RJ, Kowal EJK, Buchthal J, Housden BE, Perrimon N, Collins JJ, Church ...

10. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type

    Blog Post

    Published

    July 23, 2020, 1:20 p.m.

    ...that have the gene deletion could replicate when challenged with the S. islandicus CRISPR-Cas system. 5. ...

11. Plasmids for Endogenous Gene Tagging in Human Cells

    Type

    Blog Post

    Published

    April 6, 2017, 1:02 p.m.

    ...Institute website at alleninstitute.org. A classic challenge in cell biology is making sure that what we observe...

12. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    ... (budding yeast), S. pombe (fission yeast), A. thaliana (mustard weed), O. cuniculus (rabbit), synthetic...

13. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type

    Blog Post

    Published

    April 6, 2021, 12:30 p.m.

    ...DNA Extracts. Pooled libraries are a bit more challenging to QC by NGS as they are often quite different...

14. Hot Plasmids and Viral Preps - January 2021

    Type

    Blog Post

    Published

    Jan. 5, 2021, 2:15 p.m.

    ... is only ~70kDa, with a molecular weight roughly half of Cas9 or Cas12a. This is in part due to the fact...

15. Adapting Toehold Switches to Detect Zika Virus

    Type

    Blog Post

    Published

    Aug. 30, 2016, 2:30 p.m.

    ...symptoms, including the development of fetal microcephaly and Guillain-Barré syndrome. In response, the...

16. The Materials Science of Optogenetics Experiments

    Type

    Blog Post

    Published

    Sept. 17, 2015, 2:30 p.m.

    ...validation experiments we discussed last week are only half of the battle when using optogentics to answer a...

17. Experimenting in an Open Source lab: from CRISPR to Cats

    Type

    Blog Post

    Published

    July 23, 2015, 2:30 p.m.

    ... case.   Funding Makerspaces One of the main challenges when starting a new project at a makerspace is...

18. Hot Plasmids: Winter 2025

    Type

    Blog Post

    Published

    March 11, 2025, 1:15 p.m.

    ... developed in human cells, has been uniquely challenging to apply in dicotyledonous plants, or dicots....

19. The AAVantages of AAV in CRISPR Screens

    Type

    Blog Post

    Published

    Feb. 4, 2025, 2:15 p.m.

    .... V., Teyssier, N., Pan, A., Danner-Bocks, S., Sanghal, T., Yadanar, L., Tian, R., Ma, K., Palop, J. J...

20. Production of Virus in Insect Versus Mammalian Cells

    Type

    Blog Post

    Published

    Nov. 5, 2024, 8:32 p.m.

    ...Come From and What Do They Mean? Overcoming the Challenges of Lentivirus Production Tips for Titering Your...