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We narrowed to 615 results for: LIS

Showing: 281 - 300 of 615 results

  1. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...as dot blot, enzyme-linked immunosorbent assay (ELISA) (Grimm et al., 1999), quantitative PCR (qPCR) (...blog post.  We generally do not recommend using ELISA-based titering assays, since those will quantify...Titration of AAV-2 particles via a novel capsid ELISA: Packaging of genomes can limit production of recombinant...

  2. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ... for you and your goals. Making a List of Schools Creating a list of schools is one of the first steps.... With virtual options, you can narrow down your list of schools by meeting with professors - a/k/a Principle...

  4. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...also been a great place to reach out to local establishments such as museums, professional organizations... myidp.sciencecareers.org mentioned museums. Indianapolis is home to one of "The world’s biggest and best...children’s museum", The Children's Museum of Indianapolis. I drove passed these dinosaurs every day. They...

  5. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...techniques. More and more CRISPR tools are being published each month, making CRISPR a great choice for your...to consult the literature to see if anyone has published a protocol that would work for your system. Addgene... are a few plasmids at Addgene that have been published as CRISPR testing tools: Traffic Light Reporter...

  6. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...cistron will start with the Proline.  The table below lists the four common 2A peptides employed by scientists...to express two or more genes, some of which are listed below. We should note that these vectors are primarily...gene or genes of interest. Some of the plasmids listed in the table above (and their related plasmids)...

  7. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    .... What the BioBricks Foundation is seeking to accomplish is to make the engineering of biology much easier...like this conversation we are having now is in English, and then there are languages to communicate with.... The question is who owns languages? Who owns English? Well, everybody does; it's free to use and that...

  8. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...some accountants running our math tables!  Be realistic about what you can teach  5-10 year olds in a ...for 8th graders was a little intimidating. Too simplistic and I would insult the students. Too complicated...them had imagined for a scientist. I think that establishing this history helped demonstrate that a) I was...

  9. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...Funded by the NSF (with Co-PIs Jessica Bell, Lisa Gentile, Ellis Bell, and Amy Springer), this group now connects...Molecular Biology Annual Meeting. Some of my students published their work identifying new genes required for ...

  12. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...or monster.com, where you’ll find a variety of listings. Post your CV and make it visible, which will ...in a recruiter giving you a call. Many companies enlist the assistance of recruiters for identifying appropriate...know very well; always ask for information and establish a connection with that person before ever asking...

  13. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...people or discussing science, it is a great way to establish yourself as a thought leader and have real conversations...on subreddits (r/”topic”) which are essentially lists of forums on particular topics. Volunteer moderators...moderators were on top of any comments that were at all trollish. Kudos to the moderators for keeping the quality...

  14. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...Line Authentication Committee includes a database listing all cross contaminated or misidentified cell lines...cell line provenance and identity. The Nature Publishing Group recently reiterated its strict stance on...immortal life of Henrietta Lacks. New York: Crown Publishers, 2010. 2. Gold, Michael. "A conspiracy of cells...

  15. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ... a powerful laboratory tool often employed to establish cell lines that stably express a gene of interest...mycoplasma detection kits that use detector cell lines, ELISA, fluorometry, or PCR-based approaches to detect ...possible transduction. Be consistent Once your lab establishes the best conditions for specific applications...

  16. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...tables below listing some of the most common bacterial and mammalian promoters. These lists is by no means...on its orientation to your gene Although this list is a great place to start, the tables above do not...

  17. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...The following is a description of a technique published by Simpson, Sacher, and Szymanski in January 2016... for cell-binding abilities (6). This can be accomplished using a restriction enzyme with a short recognition...PubMed PMID: 26275959. 2. Weigele, Peter, and Elisabeth A. Raleigh. "Biosynthesis and Function of Modified...

  18. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    Published
    Nov. 6, 2015, 3:30 p.m.
    ...were Spanish scientists (MD, PhD and MD/PhD) established in some of the top Universities in USA and Europe...laboratories to start working on projects together. List of discussion topics at IMP In these sessions, mentors... Certified neurologist and multiple sclerosis specialist, currently President and owner of THiNKBrain,...

  19. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...contributed by guest blogger Eleanor Wang, a Research Specialist in Patrick Hsu's lab at UC Berkeley. Most of ... As scientists, we probe beyond the realm of established theory. Realizing that textbooks don’t tell us...blogger! Eleanor Wang is currently a Research Specialist in Patrick Hsu’s lab at UC Berkeley. She will...

  20. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...used existing BRAF gRNA viruses, but you can find a list of validated gRNA plasmids on our website. There...amplified a 500-600 bp product. The primers I designed (listed above) worked well and I got clear bands using ... I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, Root DE. Nat Biotechnol. 2016 Jan 18. doi:...
Showing: 281 - 300 of 615 results