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We narrowed to 712 results for: Met

Showing: 281 - 300 of 712 results

  1. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ... remind you of anything? Perhaps the Scientific Method? Imagine your problem is just a question you have... skills” is the ability to apply the scientific method to problems in a variety of contexts.  How do you...skills? Thankfully, as scientists, the scientific method is a core part of our academic training.  Developing...their logic. Or you can try applying the scientific method to everyday problems in your life. This tactic ...

  2. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    Published
    May 5, 2015, 3:54 p.m.
    ...is sifting through the long list of titles for something that is actually useful to you. When embarking... the jargon and background in your field, but sometimes papers are also just poorly written. By nature... or are trying to replicate an experiment, the methods section probably won’t get much attention reading...of the appropriate controls? Refer back to the methods section for finer points of the protocols. Are ...

  3. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...repeats separated by an 8-bp long asymmetric core spacer sequence. The asymmetry in the core sequence gives ...specified times. Combining this with some of the loxP methods described above, a genetic modification can be ...site have been created, which contain a unique asymmetric spacer "NNNTANNN", where "N" indicates which ...

  4. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...more than one protein in your sample? Or what if something weird happened during your western and your results...antibodies, especially with more stringent stripping methods. If you are planning to detect multiple proteins... most PVDF membranes recommend re-wetting with methanol before rinsing with wash buffer, as dry PVDF won... that has been dried, no matter what stripping methods you throw at it. Ideally, dry your membrane once...

  5. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    Published
    Aug. 20, 2019, 12:31 p.m.
    ...beginning. Variability source Standardization parameters Animal Strain, age, sex, housing conditions...reduce expression levels of certain genes. A popular method uses Cre recombinase on transgenic flox animals... area of interest may be very limited with this method, especially in adult animals, and not targeted ...order to maximize possible effects. Injection Parameters: The injection needle should be as thin as possible...

  6. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ... tool in the field as it provides a noninvasive method for manipulating cell activity, especially neuronal... a chemogenetic system, you’ll need to choose a method for targeting receptor expression as a ligand and...expression must be restricted to specific circuits. Methods for targeting expression involve tissue specific...as a DREADD agonist due to its ability to be metabolized into clozapine (CLZ). While some scientists argue...

  7. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...pieces of the FP back together (Figure 1). This method is generally known as Bimolecular Fluorescence ...GFP reassembly is a fast, efficient, and robust method for detecting protein-protein interactions in vivo...options, check out the 2in1 Kit for FRET and ratiometric BiFC from Christopher Grefen’s lab or explore...Remember that the FP fragments themselves can sometimes interact in cells and produce background signal...

  8. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...the laser and measuring its output with a light meter before and after polishing. Efficiency of the implant...series already provided or check the materials and methods of a paper that uses the variant you wish to use... pulses. An example of a standard stimulation parameter is 20Hz (laser output), 5ms “on”, 50ms “off”.  ...lasers are also available. Optimal stimulation parameters must also be determined empirically but it’s ...

  9. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...screens can be carried out using a few different methods — in vivo, in vitro, and ex vivo. The general process..., so having an efficient and effective delivery method is vital for the best outcomes. The go-to in vivo...vivo option AAV has become the preferred delivery method for in vivo CRISPR screens. AAV vectors can be ...that is unmatched by other viral vector delivery methods. Lentivirus has limited packaging options, with...

  10. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...company is “…where molecules become real”.  We sometimes forget that the models we create are around 30...interrogation process, they will almost invariably say something like “Oh,…I didn’t quite appreciate that….” For...determined electron density, have been known to say something silly like “See there, arginine 53 really is right...them to slow down and focus their attention on something tangible. Under the guidance of a trained educator...

  11. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    Published
    July 13, 2017, 2:30 p.m.
    ...mother. Inside the pupal case, the larva will metamorphose into its adult form. In all, it takes roughly...homozygous alleles, others cannot. Geneticists need a method for maintaining stable lines of genetic mutants...recombine chromosomes during meiosis. This can sometimes be a handy feature when setting up genetic crosses...symbol”. Additional Resources on the Addgene Blog Methods for Site Specific Recombination in Drosophila Learn...

  12. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ... genome engineering parameters via a library-on-library approach." Nature methods 12.9 (2015): 823-826...RNA-guided gene regulation and editing." Nature methods 10.11 (2013): 1116-1121. PubMed PMID: 24076762....genome editing, activation and repression." Nature methods 12.11 (2015): 1051-1054. PubMed PMID: 26344044....

  13. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...(Anzalone et al., 2019; Chen & Liu, 2023). This method uses a prime editor (typically a Cas9 H840A nickase...pegRNAs in one experiment (Figure 1). Through this method, we found key determinants of prime editing efficiency...Koeppel et al., 2023), their predictive power is sometimes limited across different targets due to variations...Parallel; Akhtar et al., 2013; Schep et al., 2021) method to link prime editing efficiency to chromatin data...

  14. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ...engineered nucleases. Nat Methods 11:1051–1057 . https://doi.org/10.1038/nmeth.3075 Lukashev AN, Zamyatnin...Scientific Reports paper introducing the delivery method in 2014, Manuel Goncalves lab report that AdV-mediated...lab found in a second paper published in Nature Methods the same year that delivering RNA-guided nucleases...

  15. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...grad student, I thought that “negotiation” was something that I would mainly do over the price of a used...unprepared. Mainly, I thought of negotiation as something done not by people wearing a lab coat, but instead... argument but felt bad afterwards). Or worse, sometimes there’s no agreement at all and everyone leaves...example provides a scenario where both people get something valuable. Importantly, as the third example shows...

  16. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...yields by stabilizing rate-limiting enzymes. In this method, the major capsid protein VP1 is co-expressed with...Cheah et al., 2021.   After validating this method through monitoring GFP degradation, this protein...acid, suggesting that MPyV system can improve metabolic pathway yields by encapsulating a single enzyme...pathway, the MPyV system provides a simple, efficient method for protecting a single enzyme that drives biosynthetic...

  17. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...luminescence, which require UV lamps, fluorometers, or luminometers, chromoprotein detection can be done...taken up the plasmid. Of course, other follow up methods like sequencing are more definitive, but visual...before taking on more time consuming and expensive methods. While techniques such as  blue-white screening...

  18. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...Yamamoto’s lab has harnessed MMEJ to create a new method for CRISPR gene knock-in, termed PITCh (Precise... MMEJ: An Introduction There are three primary methods for repairing DNA after a double-stranded break.... These results indicate that PITCh is a robust method for genomic insertion. PITCh can also be adapted...where HDR is downregulated, MMEJ represents another method for making targeted modifications. A recent publication...

  19. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...switching to Golden Gate is a conceptual barrier. Sometimes people don’t know how to start. They might want...Most Sainsbury scientists are active on Twitter. Sometimes it’s a good way to find out what’s happening in...experiences, articles about women in science, and the sometimes stupid or uncomfortable things that have happened...plant science. I think their chances of doing something amazing will be equally high as for those who ...

  20. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ... BLAST button, consider the Program Selection parameter, as this will affect the amount of time to perform...BLAST button, you may have noticed the “Algorithm parameters” link. Click on this link to view advanced BLAST...such as when you attempt to verify the starting methionine of a gene but the blastx results start the alignment...have to remember to adjust any blastp algorithm parameters. We use the default scoring matrix BLOSUM62, ...
Showing: 281 - 300 of 712 results