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We narrowed to 849 results for: NTS

Showing: 281 - 300 of 849 results

  1. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...are going to be there – undergraduate students, rotation students, joining a new lab at any career stage... help clean up and/or make necessary reagents for the experiments. Unlike in lab courses, where everything...double-check you remember where the equipment, reagents, and samples are. Do: Ask your bench mentor if...everything is magically made up by helpful TAs or assistants, and sitting out on the bench waiting for you...with…now”. Ask for feedback so you can make adjustments. This could look like “What are some things I...

  2. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...minutes. While users can’t tangibly see these improvements, you are benefited with reassurance your plasmids...on stories during short periods of time called sprints. The team is mindful of the number of stories we...without impacting the overall work. Each of our sprints is themed and the stories in that sprint relate.... Third party libraries are reusable software components that can be incorporated into software. You can... can think of these libraries as ingredients to a recipe. It’s unrealistic to plant, grow, and harvest...cake depends on how you combine it with other ingredients.  For example, we use a third party library to...over again for every single software today that prints labels. These libraries in turn need to stay up...

  3. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    .... This enhanced movement is thought to reflect elements of drug addiction and craving (‘seeking drugs’...approach against drug addiction. Anxiety tests Rodents have a natural preference to stay in dark areas...ones (risky). They also prefer to explore novel environments by initially moving along a wall (protected)...Taking advantage of these characteristic traits, rodents can be used to study anxiety levels in general,... driven by their motivation to explore novel environments, rather than being inhibited by fear/anxiety...test the efficacy of antidepressant treatment in rodents. In this test, an animal is placed into a tank ... of microRNA124a in the hippocampus evokes antidepressants-like effects in rats." Psychoneuroendocrinology...

  4. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...feeling out of their lab, but for many graduate students and postdocs, the people you see in your bay for... a lab. You formulate hypotheses and conduct experiments. You transfer liquids between tubes, look through...office transition? I’ve already provided a few counterpoints to some of the causes of culture shock above...it’s like to be properly onboarded. Most work environments outside of academia provide a formal training...probably because you don't have to worry about experiments taking longer than expected or dealing with unexpected... bring real scientists into schools to provide students with hands-on lab opportunities. You can get your...are there once you start looking for them. Acknowledgements Big thank you to Jessica Welch, Mike Lemieux...

  5. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...option, try the Biospace career events page. Biospace holds career events all over the country; sometimes... thereof) One of the biggest challenges facing students today is that many graduate and post-doctoral ... development training. Further, even for those students and researchers who do take the initiative to ...obviously important experience, but far too many students and young professionals will never attend a job...which are larger meetings offering resources for students seeking employment within industry or academia...using ResearchGate to see where other graduate students and post-docs from your institution have gone,...

  6. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...it's time to take a look at some of its parts. Elements such as the antibiotic resistance gene and the...polymerase binding site) and operators (response elements). Promoters are about 100 to 1000 base pairs long...RNAP can recognize and bind to specific promoter elements. This means that the promoter present in your ...various eukaryotic cell types (mammalian, yeast, plants, etc) require unique promoters and there is very... other regulatory factors: enhancers, boundary elements, insulators, and silencers; however, some "leakiness...typically include some combination of other promoter elements, and tend to be more tightly regulated. Common...ubiquitin gene Constitutive  Gives high expression in plants. H1 small RNA expression shRNA From the human...

  7. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    Published
    Dec. 18, 2018, 1:17 p.m.
    ...genome editing in plants While there have been many Cas nucleases developed for plants, it has been difficult...Encyclopedia of DNA Elements (ENCODE), a consortium aimed to identify functional elements in the human genome...specificity of multiple wild-type and engineered variants of Cas nucleases. In doing so, they also created... toolkit for Cas-mediated genome engineering in plants. Read the paper in bioRxiv Find the plant genome...

  8. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    Published
    Sept. 2, 2014, 3:05 p.m.
    ...and Harvard. Students also partner with independent career organizations to get the grants and programs...recently appeared in eLife: "A Fair Deal for PhD Students and Postdocs" by Dr. Henry Bourne, Professor Emeritus...proactive role in their non-bench training. Grad students at Washington University founded a successful ... enough. There are still university research departments that have no formal career resources for science...science trainees at all. For grad students and postdocs interested in taking matters into their own hands...

  9. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    ... single enzyme to cut fragments that are very different.” Because the fragments produced will have different... in vitro; it’s no different in mouse, human or plants,” explained Jan Lohmann of Heidelberg University...GreenGate includes the six most frequently used elements in plant expression cassettes: plant promoters...resistance cassettes for selection of transgenic plants. The complete GreenGate toolkit, now available ...required to generate multi-construct transgenic plants. In the most practical terms, what GreenGate can...

  10. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...TALEs), with fifteen total variants deposited with Addgene. These variants, including a “miniCRISPR” version...than using more complex protective methods or detergents like CHAPSO, while requiring a lower concentration...RvLEAMshort to the sample before plunge freezing represents a convenient, effective approach to protecting...other viral delivery methods, allowing all the PE components to be delivered in a single package. In a recent...latest work evolves and engineers Bxb1 recombinase variants to optimize PASSIGE efficiency (Pandey et al.,...allowing the team to develop self-silencing CHARM variants that stop their own expression after silencing...

  11. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ... and the linkage of intra- and extracellular environments. Found on nearly every cell in the human body...crucial for their function. With the right research reagents, researchers can measure this conformational change...lab, and IPI — used IPI antibodies and affinity reagents to study the difference in cell morphology changes... teams employed IPI synthetic antigen-binding fragments (Fabs) that bound an amino acid region displayed...containing the αV subunit. Using these affinity reagents, the investigators inhibited other RGD-binding...collaboration and education in protein science, IPI hosts events and training courses on technologies and laboratory... on the IPI website and stay informed on future events by signing up for the IPI newsletter. The collection...

  12. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...expression in strains of bacteria that are redox mutants so they have a more oxidizing cytoplasm. expression...intensity of a tagged protein. SunTag has two components: 1) a protein of interest that’s tagged with ...inflammatory therapeutic applications. Antibody fragments such as scFvs are also used for eye diseases, ... Function, and Clinical Prospects of Antibody Fragments. Antibodies 8:28 . https://doi.org/10.3390/antib8020028...Applications of Single Chain Fv (Variable Domain) (scFv) Fragments. Antibodies 2:193–208 . https://doi.org/10.3390... Honegger A, Plückthun A (1998) Antibody scFv fragments without disulfide bonds, made by molecular evolution...Expression System in Producing Antibody Recombinant Fragments. IJMS 21:6324 . https://doi.org/10.3390/ijms21176324...

  13. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...our understanding of biological processes and developments. Model organisms often feature genetic, physiological...candidate for introductory teaching and research environments (Idalia & Bernardo, 2017).  Escherichia...stroke and hypertension (Iannaccone et al., 2009). Rodents have also been routinely used in an array of neuroscience...expected to shed light on human aging pathological developments (Vieira et al., 2019) Besides their ability ...popular tools for studying frogs include egg transplants and microinjections. Injections of mRNAs, for...Addgene’s plasmid collections to help start your experiments! References: Blount, Z. D. (2015). The unexhausted... A., Wells, K. M., & McCusker, C. D. (2019). Advancements to the Axolotl Model for Regeneration and Aging...

  14. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    Published
    July 21, 2015, 2:30 p.m.
    ...to the start of another academic year, graduate students and post-docs alike are wondering where the time...tangible research and career goals, and celebrate accomplishments. These techniques are laid out in Vincent et...conversation. Goals and Planning Worksheet: Lists accomplishments, research/professional goals and feedback. ...goals and effort needed to complete them. These documents dictate the subject matter discussed at the meeting...have accomplished. Starting with a recap of accomplishments, from learning new techniques to finally figuring...development - let us know your strategies in the comments section!   References 1. Yearly Planning Meetings...

  15. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type
    Blog Post
    Published
    Nov. 27, 2019, 2:03 p.m.
    ...are many combinations to choose from for your experiments! Late last year, we hosted our first AAV Education... main takeaways from the paper for designing experiments with AAV: 1. Getting your viral vector into your... nontransgenic animals by using cis regulatory elements such as promoters and enhancers, multicistronic...multicistronic vectors, and post-translational regulatory elements. Examples of these approaches are given in Tables...development of this practical guide by the workshop participants  - a testament to openness and sharing in science...the neuroscience community by sharing not only reagents, but also method information. To complement these...

  16. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...different points in the pathway. Photoswitches are great for teasing out important branch points along a... to know about them to be successful in your experiments? In this post, we will discuss some broad classes...flexibility in using optogenetic tools in their experiments. Some tools turn on and off rapidly with light...determine which one(s) are candidates for your experiments.  Wavelengths Most vectors have an optimal wavelength... light stimulus. This can make or break your experiments!  First, closely read the publication, including...number of factors that can wreak havoc on your experiments, so it is important to understand the details...

  17. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ... and suggest follow up experiments regularly? Can you work on some experiments of your own choosing? Does...scheduled or only ad hoc? Does the mentor keep appointments with group members or are they frequently cancelled...the department? Are they successful at getting grants and funding? Beware of bullying Your mental health...harassment or bullying. Even with the rise of movements such as the Future of Research Symposium and #...develop transferable skills by attending career events or being involved in a grad or postdoc association...there will be a way to grow and learn. Graduate students and postdocs in particular must care about this...

  18. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...methods and the types of experiments they are commonly used for.  Table 1. Points to Keep in Mind When Performing...Visualize components of individual cells Separation/identification of fluorescently-labeled components Fluorescence-activated...fluorescent protein for single and multi-color imaging experiments. Finally, what kind of system will you use to... Performing Biosensor Experiments Experiment  Detection Method Notes Quantification of a process ...

  19. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    Published
    Feb. 1, 2018, 3:07 p.m.
    ... results with reference sequences To design experiments for further plasmid verification Identifying...process for incoming plasmids can vary based on the contents of a plasmid, the first step is always to send...for next generation sequencing. We then copy the contents of the resulting FASTA file into the desktop version.... Using Snapgene for additional verification experiments: Primer and restriction digest design Some regions...These regions require additional verification experiments that can be partially designed using the Snapgene...create custom annotations and design additional experiments like restriction digests. We’ll have a tutorial...

  20. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...weeks or every month is long enough that some experiments can get done and data analyzed between each meeting... from the lab members who are conducting the experiments to the PIs. Remote meetings can be tricky, and...communication tools such as Slack. Share data and documents While having multiple scientists edit the same... slower, sequential communication of emailing documents to one person at a time. Just be sure there is...preoccupied with other work or prioritize other experiments. Therefore, it’s crucial that everyone meets ... Some things to figure out are timelines for experiments and manuscripts, who is responsible for which...
Showing: 281 - 300 of 849 results