Skip to main content
Addgene

We narrowed to 524 results for: PROC

Showing: 281 - 300 of 524 results
  1. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...You can see some of Joanne’s comments on the AMA process below, but we also wanted to share some thoughts...submission to avoid rousing the trolls. 3. The AMA process was simple and well organized. You can check out...
  2. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...doses and timings of their drug treatments. The process of rising to such challenges involves using knowledge...up-to-date information about the industry, recruiting processes, and can connect you to a community of colleagues...
  3. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...a post that goes into the mouse genome editing process in a bit more detail in the coming weeks, but, ...Target Mouse Genes To begin the in vitro screening process, you should first develop primers to the approximately...
  4. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ... that of the 16,000 manuscripts that had been processed since the declaration, only a small fraction (...practicality (given image sizes) but images should be unprocessed and in capture format. This raises another issue...
  5. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ... that drain resources from the actual research process. It does take time and effort to implement an ELN...organize their work at every step of the research process will save them significant amounts of time and ...
  6. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...communicated. The next step is to come up with a viable process that will avoid such miscommunications in the future...and examples that have guided your resolution processes in the past. Types of Resolution There are of...
  7. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...is displayed on the coats of bacteriophages, a process which links genotype and phenotype. Incubating ...single-chain Fv analogue produced in Escherichia coli. Proceedings of the National Academy of Sciences 85:5879–5883...
  8. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...frequency of each variant during the selection process. Each variant is assigned a functional score based...library frequency after selection. Key to this process is the ability to generate full libraries of mutant...
  9. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...for biological spectroscopy and microscopy." Proceedings of the National Academy of Sciences 114.23 (2017... real-time multicolor luminescence imaging." Proceedings of the National Academy of Sciences (2015): 201418468...
  10. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...looking for, you are ready to begin the screening process. Want to set yourself up for a screening success...then a single, bulk validation may be enough to proceed with your experimental plan. Validating knockout...
  11. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ... mature tissues - no problem, as exon skipping proceeds through the non-homologous end joining (NHEJ) ...is upregulating HDR in mature tissues, as this process is necessary to precisely edit point mutations....
  12. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...value that helps you determine whether or not to proceed with a used or new item. To further illustrate ...we mentioned previously.  There is value in the process of creating a budget; you can share your budget...
  13. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...GPCR signaling GPCR signaling is a complicated process involving many downstream signaling mechanisms ...light-gated cation-selective membrane channel. Proceedings of the National Academy of Sciences, 100(24),...
  14. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...affect expression, before starting the selection process. Factors to think about are (1) your experimental...will allow you to efficiently move through this process.  Many other factors can also affect your AAV experiments...
  15. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ... appropriate secondary antibodies. The general process is similar to standard immunofluorescence, but ...spatial analysis of cells in complex tissues. Proceedings of the National Academy of Sciences, 117(52),...
  16. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...template. HDR, however, is a very low-efficiency process, and usually involves the need for single cell ...successful edits. This is a very time consuming process and should not be undertaken lightly! Indeed, truly...
  17. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...useful for the biotech industry, which is in the process of capitalizing on phage RBPs as diagnostic tools...DNA fragments with live host cells Now you can proceed with incubating this membrane with your cells of...
  18. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...not always guaranteed. To learn more about this process check out our Mouse Modeling blog posts: Part 1...have germline competent ESC lines. Second, the process is much quicker. It takes 3 months to generate ...
  19. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ... controlled. I worked from Standard Operating Procedures (SOP’s) and every item I tested was constantly...the technology is so advanced that most of the procedures in the lab are done with robots. Young scientists...
  20. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...incorporation of that sequence into the zygote’s genome, a process that’s highly unpredictable. Transposons, however...injection into the cytoplasm of a fertilized egg, a process which is inefficient when DNA is injected. Sleeping...
Showing: 281 - 300 of 524 results